Multiplex real-time PCR SYBR Green for detection and typing of group III Clostridium botulinum

Veterinary Microbiology

Volumn 154, Issue 3-4, 2012, Pages 332-338

  Anniballi, Fabrizio ^a   Auricchio, Bruna ^a   Delibato, Elisabetta ^a   Antonacci, Monia ^a   De Medici, Dario ^a   Fenicia, Lucia ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

Animal botulism; Clostridium botulinum group III; Detection method; In house validation; Mosaic neurotoxin; Real time PCR SYBR Green

Indexed keywords

BACTERIAL DNA;

ACCURACY; ARTICLE; BACTERIUM IDENTIFICATION; CLOSTRIDIUM BOTULINUM TYPE C; CLOSTRIDIUM BOTULINUM TYPE D; CONTROLLED STUDY; LIMIT OF DETECTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROCESS DEVELOPMENT; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS;

ANIMALS; BACTERIAL TYPING TECHNIQUES; CLOSTRIDIUM BOTULINUM; ORGANIC CHEMICALS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALIA; CLOSTRIDIUM BOTULINUM;

EID: 84855949895     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2011.07.018     Document Type: Article

References (20)

1. Abdulmawjood A., Roth S., Bulte M. Two methods for construction of internal amplification controls for the detection of Escherichia coli 0157 by polymerase chain reaction. Molecular and Cellular Probes 2002, 16:335-339.
2. Allison M.J., Maloy S.E., Matson R.R. Inactivation of Clostridium botulinum toxin by ruminal microbes from cattle and sheep. Applied and Environmental Microbiology 1976, 32:685-688.
3. CDC Botulism in United States, 1899-1996. Handbook for Epidemiologists, Clinical and Laboratory Workers 1998, Centers for Diseases Control and Prevention.
4. Chaffer M., Baum M., Grinberg K., Molad T., Elad D. Application of PCR for detection of Clostridium botulinum type D in bovine samples. Journal of Veterinary Medicine, Series B 2006, 53:45-47.
5. Deprez P.R. Tetanus and botulism in animals. Clostridia in Medical, Veterinary and Food Microbiology - Diagnosis and Typing 2006, 27-36. European Commission, Luxembourg. J. Mainil (Ed.).
6. Edwards M.C., Gibbs R.A. Multiplex PCR: advantages, development, and applications. Genome Research 1994, 3:S65-S75.
7. Fach P., Gibert M., Griffais R., Popoff M.R. Investigation of animal botulism outbreaks by PCR and standard methods. FEMS Immunological and Medical Microbiology 1996, 13:279-285.
8. Fenicia L., Anniballi F., De Medici D., Delibato E., Aureli P. SYBR Green real-time PCR method to detect Clostridium botulinum type A. Applied and Environmental Microbiology 2007, 73:2891-2896.
9. Franciosa G., Fenicia L., Caldiani C., Aureli P. PCR for detection of Clostridium botulinum type C in avian and environmental samples. Journal of Clinical Microbiology 1996, 34:882-885.
10. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Research 1996, 6:986-994.
11. International Organization for Standardization (ISO) EN ISO 16140. Microbiology of Food and Animal Feeding Stuffs - Protocol for the Validation of Alternative Methods 2003.
12. Lindberg A., Skarin H., Knutsson R., Blomqvist G., Båverud V. Real-time PCR for Clostridium botulinum type C neurotoxin (BoNTC) gene, also covering a chimeric C/D sequence - application on outbreaks of botulism in poultry. Veterinary Microbiology 2010, (Epub ahead of print).
13. Nakamura K., Kohda T., Umeda K., Yamamoto H., Mukamoto M., Kozaki S. Characterization of the D/C mosaic neurotoxing produced by Clostridium botulinum with bovine botulism in Japan. Veterinary Microbiology 2010, 140:147-154.
14. Peck M.W. Biology and genomic analysis of Clostridium botulinum. Advanced in Microbial Physiology 2009, 55:184-265. 320.
15. Prévot V., Tweepenninckx F., Van Nerom E., Linden A., Content J., Kimpe A. Optimization of polymerase chain reaction for detection of Clostridium botulinum type C and D in bovine samples. Zoonose Publich Health 2007, 54:320-327.
16. Rodrìguez-Lázaro D., Hernández M., Esteve Hoorfar J., Pla M. A rapid and direct real time PCR-based method for identification of Salmonella spp. Journal of Microbiological Methods 2003, 54:381-390.
17. Szabo E.A., Pemberton J.M., Gibson A.M., Thomas R.J., Pascoe R.R., Desmarchelier P.M. Application of PCR to a clinical and environmental investigation of a case of equine botulism. Journal of Clinical Microbiology 1994, 32:1986-1991.
18. Takeda M., Tsukamoto K., Kohda T., Matsui M., Mukamoto M., Kozaki S. Characterization of the neurotoxin produced by isolates associated with avian botulism. Avian Diseases 2005, 49:376-381.
19. Takeda M., Kasai H., Torii Y., Mukamoto M., Kohda T., Tsukamoto K., Kozaki S. Protective effect of botulinum C/D mosaic toxoid against avian botulism. Journal of Veterinary Medical Science 2006, 68:325-330.
20. Williamson J.L., Rocke T.E., Aiken J.M. In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay. Applied and Environmental Microbiology 1999, 65:3240-3243.