Isolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions

Oncology Reports

Volumn 30, Issue 3, 2013, Pages 1143-1148

  Massani, Marco ^a   Stecca, Tommaso ^a   Fabris, Luca ^b   Caratozzolo, Ezio ^a   Ruffolo, Cesare ^a   Furlanetto, Alberto ^a   Morton, Stuart ^b,c   Cadamuro, Massimiliano ^c   Strazzabosco, Mario ^d   Bassi, Nicolò ^a  

^a Treviso Hospital   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

^c UNIVERSITY OF MILANO BICOCCA   (Italy)

^d YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cell isolation; Cholangiocarcinoma; Stromal cells; Tumor stroma interactions

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; CD68 ANTIGEN; CELL MARKER; CYTOKERATIN 19; CYTOKERATIN 7; VIMENTIN;

AGED; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BILE DUCT CARCINOMA; BILIARY EPITHELIUM CELL; CANCER CELL; CANCER SURGERY; CELL INTERACTION; CELL ISOLATION; CLINICAL ARTICLE; COCULTURE; EPITHELIUM CELL; FEMALE; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; LIVER PARENCHYMA; MALE; MESENCHYMAL STROMA CELL; NONHUMAN; PRIMARY CELL CULTURE; PRIORITY JOURNAL; RAT; TUMOR CELL;

BILE DUCT NEOPLASMS; BILE DUCTS, INTRAHEPATIC; CELL COMMUNICATION; CHOLANGIOCARCINOMA; COCULTURE TECHNIQUES; EPITHELIAL-MESENCHYMAL TRANSITION; FIBROBLASTS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; IMMUNOENZYME TECHNIQUES; IMMUNOMAGNETIC SEPARATION; NEOPLASM GRADING; STROMAL CELLS; TUMOR CELLS, CULTURED; TUMOR MARKERS, BIOLOGICAL;

EID: 84880646407     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2013.2568     Document Type: Article

References (26)

1. El-Serag HB and Rudolph KL: Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology 132: 2557-2576, 2007.
2. Aljiffry M, Walsh MJ and Molinari M: Advances in diagnosis, treatment and palliation of cholangiocarcinoma: 1990-2009. World J Gastroenterol 15: 4240-4262, 2009.
3. Deoliveira ML, Schulick RD, Nimura Y, Rosen C, Gores G, Neuhaus P and Clavien PA: New staging system and a registry for perihilar cholangiocarcinoma. Hepatology 53: 1363-1371, 2011.
4. Khan SA, Thomas HC, Davidson BR and Taylor-Robinson SD: Cholangiocarcinoma. Lancet 366: 1303-1314, 2005.
5. Sempoux C, Jibara G, Ward SC, Fan C, Qin L, Roayaie S, Fiel MI, Schwartz M and Thung SN: Intrahepatic cholangiocarcinoma: new insights in pathology. Semin Liver Dis 31: 49-60, 2011.
6. Mueller MM and Fusenig NE: Friends or foes - bipolar effects of the tumour stroma in cancer. Nat Rev Cancer 4: 839-849, 2004.
7. Kalluri R and Zeisberg M: Fibroblasts in cancer. Nat Rev Cancer 6: 392-401, 2006.
8. Sirica AE: The role of cancer-associated myofibroblasts in intrahepatic cholangiocarcinoma. Nat Rev Gastroenterol Hepatol 9: 44-54, 2011.
9. Fabris L, Cadamuro M, Moserle L, Dziura J, Cong X, Sambado L, Nardo G, Sonzogni A, Colledan M, Furlanetto A, Bassi N, Massani M, Cillo U, Mescoli C, Indraccolo S, Rugge M, Okolicsanyi L and Strazzabosco M: Nuclear expression of S100A4 calcium-binding protein increases cholangiocarcinoma invasiveness and metastasisation. Hepatology 54: 890-899, 2011.
10. Holt AP, Haughton EL, Lalor PF, Filer A, Buckley CD and Adams DH: Liver myofibroblasts regulate infiltration and positioning of lymphocytes in human liver. Gastroenterology 136: 705-714, 2009.
11. Folkman J: Fundamental concepts of the angiogenic process. Curr Mol Med 3: 643-651, 2003.
12. Albini A and Sporn MB: The tumour microenvironment as a target for chemoprevention. Nat Rev Cancer 7: 139-147, 2007.
13. Garin-Chesa P, Old LJ and Rettig WJ: Cell surface glycoprotein of reactive stromal fibroblasts as a potential antibody target in human epithelial cancers. Proc Natl Acad Sci USA 87: 7235-7239, 1990.
14. Xing F, Saidou J and Watabe K: Cancer associated fibroblasts (CAFs) in tumor microenvironment. Front Biosci 15: 166-179, 2010.
15. Sirica AE, Campbell DJ and Dumur CI: Cancer-associated fibroblasts in intrahepatic cholangiocarcinoma. Curr Opin Gastroenterol 27: 276-284, 2011.
16. Nishihara Y, Aishima S, Hayashi A, Iguchi T, Fujita N, Taketomi A, Honda H and Tsuneyoshi M: CD10+ fibroblasts are more involved in the progression of hilar/extrahepatic cholangiocarcinoma than of peripheral intrahepatic cholangiocarcinoma. Histopathology 55: 423-431, 2009.
17. Chuaysri C, Thuwajit P and Paupairoj A: Alpha-smooth muscle actin-positive fibroblasts promote biliary cell proliferation and correlate with poor survival in cholangiocarcinoma. Oncol Rep 21: 957-969, 2009.
18. Utispan K, Thuwajit P and Abiko Y: Gene expression profiling of cholangiocarcinoma-derived fibroblasts reveals alterations related to tumor progression and indicates periostin as a poor prognostic marker. Mol Cancer 9: 13, 2010.
19. Campbell DJW, Dumur CI, Lamour NF, DeWitt JL and Sirica AE: Novel organotypic culture model of cholangiocarcinoma progression. Hepatol Res 42: 1119-1130, 2012.
20. Lim JH: Cholangiocarcinoma: morphologic classification according to growth pattern and imaging findings. AJR Am J Roentgenol 181: 819-827, 2003.
21. Fabris L, Strazzabosco M, Crosby HA, Ballardini G, Hubscher SG, Kelly DA, Neuberger JM, Strain AJ and Joplin R: Characterization and isolation of ductular cells coexpressing neural cell adhesion molecule and Bcl-2 from primary cholangiopathies and ductal plate malformations. Am J Pathol 156: 1599-1612, 2000.
22. Auth MK, Woitaschek D, Beste M, Schreiter T, Kim HS, Oppermann E, Joplin RE, Baumann U, Hilgard P, Nadalin S, Markus BH and Blaheta RA: Preservation of the synthetic and metabolic capacity of isolated human hepatocytes by coculture with human biliary epithelial cells. Liver Transpl 11: 410-419, 2011.
23. Cannito S, Novo E, Compagnone A, Valfrè di Bonzo L, Busletta C, Zamara E, Paternostro C, Povero D, Bandino A, Bozzo F, Cravanzola C, Bravoco V, Colombatto S and Parola M: Redox mechanisms switch on hypoxia-dependent epithelial-mesenchymal transition in cancer cells. Carcinogenesis 29: 2267-2278, 2008.
24. Bateman AC and Hübscher SG: Cytokeratin expression as an aid to diagnosis in medical liver biopsies. Histopathology 56: 415-425, 2010.
25. Mork C, van Deurs B and Petersen OW: Regulation of vimentin expression in cultured human mammary epithelial cells. Differentiation 43: 146-156, 1990.
26. Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C and Brown RA: Myofibroblasts and mechanoregulation of connective tissue remodelling. Nat Rev Mol Cell Biol 3: 349-363, 2002.