Partial purification and characterization of chiIO8, a novel antifungal chitinase produced by Bacillus cereus IO8

Journal of Applied Microbiology

Volumn 115, Issue 2, 2013, Pages 358-366

  Hammami, I ^a   Siala, R ^b   Jridi, M ^b   Ktari, N ^b   Nasri, M ^b   Triki, M A ^a  

^a INSTITUT DE L OLIVIER   (Tunisia)

^b UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Antifungal activity; Bacillus cereus; Biological control; Novel chitinase; Tomatoes

Indexed keywords

BACILLUS CEREUS; BIOCONTROL; CHROMATOGRAPHY; ELECTROPHORESIS; FUNGI; NITROGEN COMPOUNDS; PURIFICATION; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

ANTIFUNGAL ACTIVITIES; BIOLOGICAL CONTROLS; CHITINASES; ENHANCING EFFECT; NOVEL CHITINASE; PARTIAL CHARACTERIZATION; PARTIAL PURIFICATION; PROPERTY; SEED BORNE DISEASE; TOMATO;

BACTERIOLOGY;

2 DIETHYLAMINOETHANOL; AMMONIUM SULFATE; CHITINASE; CHITINASE IO8; DISINFECTANT AGENT; DODECYL SULFATE SODIUM; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; BACTERIUM; BIOCONTROL AGENT; BIOPESTICIDE; DISEASE PREVALENCE; ENZYME ACTIVITY; FUNGUS; GERMINATION; HOST-PATHOGEN INTERACTION; PH; PRECIPITATION (CHEMISTRY); PURIFICATION; SULFATE;

ALTERNARIA; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; ASPERGILLUS NIDULANS; BACILLUS CEREUS; BACTERIAL STRAIN; BACTERIUM CULTURE; BOTRYTIS CINEREA; COMPETITIVE INHIBITION; CONTROLLED STUDY; DISINFECTION; FUNGAL PLANT DISEASE; FUSARIUM; FUSARIUM SOLANI; MOLECULAR WEIGHT; NONHUMAN; PENICILLIUM; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; TEMPERATURE; VERTICILLIUM DAHLIAE;

BACILLUS CEREUS; BOTRYOTINIA FUCKELIANA; FUNGI; LYCOPERSICON ESCULENTUM;

EID: 84880586845     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12242     Document Type: Article

References (45)

1. Ajit, N.S., Verma, R. and Shanmugam, V. (2006) Extracellular chitinases of fluorescent pseudomonas antifungal to Fusarium oxysporum f. sp. dianthi causing carnation wilt. Curr Microbiol 52, 310-316.
2. Balasubramanian, N., Annie Juliet, A., Srikalavani, P. and Lalithakumari, D. (2003) Release and regeneration of protoplasts from Trichothecium roseum. Can J Microbiol 49, 263-268.
3. Bhushan, B. and Hoondal, G.S. (1998) Isolation, purification and properties of a thermostable chitinase from an alkalophilic Bacillus sp. G-11. Biotechnol Lett 20, 157-159.
4. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254.
5. Chang, W.T., Chen, Y.C. and Jao, C.L. (2007) Antifungal activity and enhancement of plant growth by Bacillus cereus grown on shellfish chitin wastes. Bioresour Technol 98, 1224-1230.
6. Chen, H.C., Hsu, M.F. and Jiang, S.T. (1997) Purification and characterization of an exo-N, N-diacetylchitobiohydrolase-like enzyme from Cellulomonas flavigena NTOU 1. Enzyme Microb Technol 20, 191-197.
7. Dahiya, N., Tewari, R., Tiwari, R.P. and Hoondal, G.S. (2005) Chitinase from Enterobacter sp. NRG4: its purification, characterization and reaction pattern. Eur J Biochem 8, 134-145.
8. Das, S., Van Dellen, K., Bulik, D., Magnelli, P., Cui, J., Head, J., Robbins, P.W. and Samuelson, J. (2006) The cyst wall of Entamoeba invadens contains chitosan (deacetylated chitin). Mol Biochem Parasitol 148, 86-92.
9. David, J.A. (2004) Fungal cell wall chitinases and glucanases. Microbiology 150, 2029-2035.
10. De la Vega, L.M., Barboza-Corona, J.E., Aguilar-Uscanga, M.G. and Ramirez-lepe, M. (2006) Purification and characterization of an exochitinase from Bacillus thuringiensis subsp. aizawai and its action against phytopathogenic fungi. Can J Microbiol 52, 651-657.
11. Driss, F., Kallassy Awad, M., Zouari, N. and Jaoua, S. (2005) Molecular characterization of a novel chitinase from Bacillus thuringiensis subsp. kurstaki. J Appl Microbiol 99, 945-953.
12. El-Tarabily, K.A., Soliman, M.H., Nassar, A.H., Al-Hassani, H.A., Sivasithambaram, K., McKenna, F. and Hardy, G.E. (2000) Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. J Plant Pathol 49, 573-583.
13. Essghaier, B., Rouaissi, M., Boudabous, A., Jijakli, H. and Najla, S.Z. (2009) Production and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26. World J Microbiol Biotechnol 26, 977-984.
14. Flach, J., Pilet, P.E. and Jolles, P. (1992) What's new in chitinases research? Experientia (Basel). Experientia 48, 701-716.
15. Ghorbel-Bellaaj, O., Manni, L., Jellouli, K., Hmidet, N. and Nasri, M. (2012) Optimization of protease and chitinase production by Bacillus cereus SV1 on shrimp shell waste using statistical experimental design. Biochemical and molecular characterization of the chitinase. Ann Microbiol 62, 1255-1268.
16. Gomes, R.C., Semedo, L.T., Soares, R.M., Soares, R.M.A., Linhares, L.F., Ulhoa, C.J., Alviano, C.S. and Coelho, R.R.R. (2001) Purification of a thermostable endochitinase from Streptomyces RC1071 isolated from a cerrado soil and its antagonism against phytopathogenic fungi. J Appl Microbiol 90, 653-661.
17. Guo, R.F., Shi, B.S., Li, D.C., Ma, W. and Wei, Q. (2008) Purification and characterization of a novel thermostable chitinase from Thermomyces lanuginosus SY2 and cloning of its encoding Gene. Agric Sci 7, 1458-1465.
18. Gupta, R., Gupta, K., Saxena, R.K. and Khan, S. (1999) Fungicidal management of root rot (Rhizoctonia solani) of French bean. Biotechnol Lett 21, 135-138.
19. Haggag, W. and Abdallh, M.E. (2012) Purification and characterization of chitinase produced by endophytic Streptomyces hygroscopicus against some phytopathogens. J Microbiol Res 2(5), 145-151.
20. Hsu, S.C. and Lockwood, J.L. (1975) Powdered chitin agar as a selective medium to enumeration of actinomycetes in water and soil. Appl Microbiol 29, 422-426.
21. Kudan, S. and Pichyangkura, R. (2009) Purification and characterization of thermostable chitinase from Bacillus licheniformis SK-1. Appl Microbiol Biotechnol 157, 23-35.
22. Kupiec, C.R. and Chet, I. (1998) The molecular biology of chitin digestion. Curr Opin Biotechnol 9, 270-277.
23. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
24. Lee, Y.G., Chung, K.C., Wi, S.G., Lee, J.C. and Bae, H.J. (2009) Purification and properties of a chitinase from Penicillium sp. LYG 0704. Protein Expr Purif 65, 244-250.
25. Lien, T.S., Yu, S.T., Wu, S.T. and Too, J.R. (2007) Induction and purification of a thermophilic chitinase produced by Aeromonas sp. DYU-Too7 using glucosamine. Biotechnol Bioprocess Eng 12, 610-617.
26. Liu, C.L., Shen, C.R., Hsu, F.F., Chen, J.K., Wu, P.T., Guo, S.H., Lee, W.H., Yu, F.W. et al. (2009) Isolation and identification of two novel SDS-resistant secreted chitinases from Aeromonas schubertii. Biotechnol Prog 25, 124-131.
27. Makino, A., Kurosaki, K., Ohmae, M. and Kobayashi, S. (2006) Chitinase catalyzed synthesis of alternatingly N-deacetylated chitin: a chitin-chitosan hybrid polysaccharide. Biomacromolecules 7, 950-957.
28. Matsushima, R., Ozawa, R., Uefune, M., Gotoh, T. and Takabayashi, J. (2006) Interspecies variation in the Kanzawa spider mite differentially affects induced defensive response in lima bean plants. J Chem Ecol 32, 2501-2512.
29. Mitsuhiro, U., Yukiko, K., Aji, S., Masami, N. and Kazutaka, M. (2005) Purification and characterization of chitinase B from moderately thermophilic bacterium Ralstonia sp. A-471. Biosci Biotechnol Biochem 69, 842-844.
30. Natarajan, K. and Ramachandra, V.M. (2010) Optimization of chitinase production from Serratia marcescens - A classical approach. Biol Segment 1, BS/1510.
31. Nawani, N.N., Kapadnis, B.P., Das, A.D., Rao, A.S. and Mahajan, S.K. (2002) Purification and characterization of a thermophilic and acidophilic chitinase from Microbispora sp. V2. J Appl Microbiol 93, 965-975.
32. Park, J.K., Morita, K., Fukumoto, I., Yamasaki, Y., Nakagawa, T., Kawamukai, M. and Matsuda, H. (1997) Characteristics of cold-adaptive endochitinase from Antarctic bacterium Sanguibacter antarcticus KOPRI 21702. Biosci Biotechnol Biochem 61, 684-689.
33. Park, H.J., Kim, D., Kim, I.H., Lee, C.E., Kim, I.C., Kim, J.Y., Kim, S.J., Lee, H.K. et al. (2009) Purification and characterization of the chitinase (ChiA) from Enterobacter sp. G-1. Enzyme Microb Technol 45, 391-396.
34. Pattan, H.D., Gothkar, A.K., Joshi, P., Chivasa, A. and Nyamudeza, P. (2001) On-farm seed priming; using participatory methods to revive and refine a key technology. Agric Syst 69, 151-164.
35. Prabavathy, V.R., Mathivanan, N., Sagadevan, E., Murugesan, K. and Lalithakumari, D. (2006) Intra-strain protoplast fusion enhances carboxymethyl cellulase activity in Trichoderma reesei. Enzyme Microb Technol 38, 719-723.
36. Purwani, E.Y., Maggy, T.S., Yaya, R., Jae, K.H. and Yu, R.P. (2004) Characteristics of thermostable chitinase enzymes from the indonesian Bacillus sp.13.26. Enzyme Microb Technol 35, 147-153.
37. Sakai, K., Yakota, A., Kurokawa, H., Mamoru, W., Mitsuaki, M. and Moriguchi, M. (1998) Purification and characterization of three thermostable endochitinases of Bacillus noble strain MH-1 isolated from chitin containing compost. Appl Environ Microbiol 64, 3340-3397.
38. Someya, N., Nakajima, M., Hirayae, K., Hibi, T. and Akutsu, K. (2001) Synergistic antifungal activity of chitinolytic enzymes and prodigiosin produced by the biocontrol bacterium Serratia marcescens strain B2 against the gray mold pathogen, Botrytis cinerea. J Gen Plant Pathol 67, 312-317.
39. Suresh, P.V. and Chandrasekaran, M. (1999) Impact of process parameters on chitinase production by an alkalophilic marine Beauveria bassiana in solid state fermentation. Process Biochem 34, 257-267.
40. Viterbo, A., Haran, S., Friesem, D., Ramot, O. and Chet, I. (2001) Antifungal activity of a novel endochitinase gene (chit36) from Trichoderma harzianum Rifai TM. Microbiol Lett 200, 169-174.
41. Waldeck, J., Daum, G., Bisping, B. and Meinhardt, F. (2006) Isolation and molecular characterization of chitinase deficient Bacillus licheniformis strains capable of deproteinization of shrimp shell waste to obtain highly viscous chitin. Appl Environ Microbiol 72, 7879-7885.
42. Wang, S.L. and Chang, W.T. (1997) Purification and characterization of two bifunctional chitinase/lysozymes extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium. Appl Environ Microbiol 63, 380-386.
43. Wang, S.Y., Moyne, A.L., Thottappilly, G., Wu, S.J., Locy, R.D. and Singh, N.K. (2001) Purification and characterization of a Bacillus cereus exochitinase. Enzyme Microb Technol 28, 492-498.
44. Wang, S.L., Lin, T.Y., Yen, Y.H., Liao, H.F. and Chen, Y.J. (2006) Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase. Carbohydr Res 341, 2507-2515.
45. Yano, S., Wakayama, M. and Tachiki, T. (2006) Cloning and expression of an alpha-1, 3-glucanase gene from Bacillus circulans KA-304: the enzyme participates in protoplast formation of Schizophyllum commune. Biosci Biotechnol Biochem 70, 1754-1763.