Purification and characterization of a thermophilic and acidophilic chitinase from Microbispora sp. V2

Journal of Applied Microbiology

Volumn 93, Issue 6, 2002, Pages 965-975

  Nawani, N N ^a   Kapadnis, B P ^a   Das, A D ^b   Rao, A S ^b   Mahajan, S K ^b  

^a UNIVERSITY OF PUNE   (India)

^b BHABHA ATOMIC RESEARCH CENTRE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVITY; GEL PERMEATION CHROMATOGRAPHY; HOT SPRINGS; METAL IONS;

ACIDIC PH; CHITINASES; ENZYMES ACTIVITY; GEL-FILTRATION CHROMATOGRAPHY; HIGHEST TEMPERATURE; METALS IONS; PH OPTIMUM; TEMPERATURE OPTIMUMS; THERMOPHILICS; WIDE PH RANGE;

PURIFICATION;

4 NITROPHENYL BETA DEXTRO N,N' DIACETYLCHITOBIOSE; ACETIC ACID DERIVATIVE; CHITIN; CHITINASE; MERCURY; METAL ION; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; FRESH WATER;

BIOTECHNOLOGY; ENZYME; EXTREMOPHILE;

ACTINOBACTERIA; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DEGRADATION; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; GEL FILTRATION CHROMATOGRAPHY; MICROBISPORA; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; SEQUENCE ANALYSIS; TEMPERATURE; BIOREACTOR; BIOREMEDIATION; ENZYMOLOGY; GEL CHROMATOGRAPHY; HEAT; ISOLATION AND PURIFICATION; METABOLISM;

ACTINOBACTERIA (CLASS); MICROBISPORA; UNCULTURED ACTINOMYCETE;

ACTINOBACTERIA; BACTERIAL PROTEINS; BIODEGRADATION, ENVIRONMENTAL; BIOREACTORS; CHITINASE; CHROMATOGRAPHY, GEL; FRESH WATER; HEAT; HYDROGEN-ION CONCENTRATION;

EID: 0036444826     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2002.01766.x     Document Type: Article

References (42)

1. Arai, A., Masuda, S., Matsuyama, A., Murakami, S. and Nakajima, M. (1998) Molecular cloning and nucleotide sequence of the pyruvate kinase gene of an actinomycete Microbispora thermodiastatica. Applied Microbiology and Biotechnology 49, 272-276.
2. Becker, B., Lechevalier, M.P. and Lechevalier, H. (1965) Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Applied Microbiology 13, 236-248.
3. Blaak, H., Schnellmann, J., Walter, S., Henrissat, B. and Schrempf, H. (1993) Characteristics of an exochitinase from Streptomyces olivaceoviridis, its corresponding gene, putative protein domains and relationship to other chitinases. European Journal of Biochemistry 214, 659-669.
4. Blaak, H. and Schrempf, H. (1995) Binding and substrate specificities of a Streptomyces olivaceoviridis chitinase in comparison with its proteolytically processed form. European Journal of Biochemistry 229, 132-139.
5. Broadway, R.M., Williams, D.L., Kain, C.W., Harman, G.E., Lorito, M. and Labeda, D.P. (1995) Partial characterization of chitinolytic enzymes from Streptomyces albidoflavus. Letters in Applied Microbiology 20, 271-276.
6. Charpentier, M. and Percheron, F. (1983) The chitin-degrading enzyme system of a Streptomyces sp. International Journal of Biochemistry 15, 289-292.
7. Gooday, G.W. (1990) Physiology of microbial degradation of chitin and chitosan. Biodegradation 1, 177-190.
8. Goodfellow, M. (1989) Maduromycetes. In Bergey's Manual of Systematic Bacteriology, Vol. 4, ed. S. T. Williams, S.T Sharpe, M.E. and Holt, J.G. pp. 2509-2551. Baltimore: Williams & Wilkins.
9. Gupta, R. Saxena, R.K. Chaturvedi, P. and Virdi, S.S. (1995) Chitinase production by Streptomyces viridificans: Its potential in fungal cell wall lysis. Journal of Applied Bacteriology 78, 378-383.
10. Hara, S. Yamamura, Y. Fujii, Y. Mega, T. and Ikenaka, T. (1989) Purification and characterization of chitinase produced by Streptomyces erythraeus. Journal of Biochemistry 105, 484-489.
11. Hsu, S.C. and Lockwood, J.L. (1975) Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Applied Microbiology 29, 422-426.
12. Igarashi, Y. Takagi, K. Kajiura, T. Furumai, T. and Oki, T. (1998) Glucosylquestiomycin, a novel antibiotic from Microbispora sp. TP-AO184: Fermentation, isolation, structure determination, synthesis and biological activities. Journal of Antibiotics (Tokyo) 51, 915-920.
13. Iverson, K.L. Bromel, M.C. Anderson, A.W. and Freeman, T.P. (1984) Bacterial symbionts in the sugar beet root maggots Tetanops myopaeformis. Applied and Environmental Microbiology 47, 22-27.
14. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
15. Lane, D.J. (1991) 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics ed. Stackebrandt, E. and Goodfellow, M. pp. 115-175. Chichester, UK: John Wiley and Sons.
16. Lechevalier, H.A. and Lechevalier, M.P. (1970) A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales ed. Prauser, H. pp. 393-405. Jena: Gustav Fisher Verlag.
17. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265-275.
18. Marchesi, J.R., Sato, T., Weightman, A.J., Martin, T.A., Fry, J.C., Hiom, S.J., Dymock, D. and Wade, W.G. (1998) Design and evaluation of useful bacterium specific PCR primers that amplify genes coding for bacterial 16S rRNA. Applied and Environmental Microbiology 64, 795-799.
19. Merril, C.R., Goldman, D. and VanKeuren, M.L. (1983) Silver staining methods for polyacrylamide gel electrophoresis. Methods in Enzymology 96, 230-239.
20. Monreal, J. and Reese, E.T. (1969) The chitinase of Serratia marcescens. Canadian Journal of Microbiology 15, 689-696.
21. Nawani, N.N. and Kapadnis, B.P. (2001) One-step purification of chitinase from Serratia marcescens NK1, a soil isolate. Journal of Applied Microbiology 90, 803-808.
22. Neugebauer, E., Gamache, B., Dery, C. and Brezezinski, R. (1991) Chitinolytic properties of Streptomyces lividans. Archives of Microbiology 156, 192-197.
23. Nielsen, M.N. and Sorensen, J. (1999) Chitinolytic activity of Pseudomonas fluorescens isolates from barley and sugar beet rhizosphere. FEMS Microbiology Ecology 30, 217-227.
24. Nonomura, H. and Ohara, Y. (1957) Distribution of actinomycetes in soil. II. Microbispora, a new genus of the Streptomycetaceae. Journal of Fermentation Technology 35, 307-311.
25. Ohno, T., Armand, S., Hata, T. et al. (1996) A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037. Journal of Bacteriology 178, 5065-5070.
26. Okazaki, K., Kato, F., Watanabe, N., Yasuda, S., Masui, Y. and Hayakawa, S. (1995) Purification and properties of two chitinases from Streptomyces sp. J-13-3. Bioscience Biotechnology Biochemistry 59, 1586-1587.
27. Reissig, J.L., Strominger, J.L. and Leloir, L.F. (1955) A modified colorimetric method for the estimation of N-acetylamino sugars. Journal of Biological Chemistry 217, 959-966.
28. Saitou, N. and Nei, M. (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406-425.
29. Sakai, K., Yokota, A., Kurokawa, H., Wakayama, M. and Moriguchi, M. (1998) Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost. Applied and Environmental Microbiology 64, 3397-3402.
30. Shirling, E.B. and Gottlieb, D. (1966) Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology 16, 313-340.
31. Staneck, J.L. and Roberts, G.D. (1974) Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Applied Microbiology 28, 226-231.
32. Stoll, V.S. and Blanchard, J.S. (1990) Buffers: Principles and practice. Methods in Enzymology 182, 24-38.
33. Svitil, A.L., Ni Chadhain, S.M., Moore, J.A. and Kirchman, D.L. (1997) Chitin degradation proteins produced by the marine bacterium Vibrio harveyi growing on different forms of chitin. Applied and Environmental Microbiology 63, 408-413.
34. Takayanagi, T., Ajisaka, K., Takiguchi, Y. and Shimahara, K. (1991) Isolation and characterization of thermostable chitinases from Bacillus licheniformis X-7u. Biochimica et Biophysica Acta 1078, 404-410.
35. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997) The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876-4882.
36. Tsujibo, H., Minoura, K., Miyamoto, K., Endo, H., Moriwaki, M. and Inamori, Y. (1993) Purification and properties of a thermostable chitinase from Streptomyces thermoviolaceus OPC-520. Applied and Environmental Microbiology 59, 620-622.
37. Tsujibo, H., Orikoshi, H. and Shiotani, K. (1998) Characterization of chitinase C from marine bacterium Alteromonas sp. strain O-7, and its corresponding gene and domain structure. Applied and Environmental Microbiology 64, 472-478.
38. Wang, S., Moyne, A., Thottappilly, G., Wu, S., Locy, R.D. and Singh, N.K. (2001) Purification and characterization of a Bacillus cereus exochitinase. Enzyme and Microbial Technology 28, 492-498.
39. Watanabe, T., Kanai, R., Kawase, T., Tanabe, T., Mitsutomi, M., Sakuda, S. and Miyashita, K. (1999) Family 19 chitinases of Streptomyces species: Characterization and distribution. Microbiology 145, 3353-3363.
40. Williams, S.T. and Robinson, C.S. (1981) The role of streptomycetes in decomposition of chitin in acidic soils. Journal of General Microbiology 127, 55-63.
41. Wilson, D.B. (1992) Biochemistry and genetics of actinomycete cellulases. Critical Reviews in Biotechnology 12, 45-63.
42. Yamada, H. and Imoto, T. (1981) A convenient synthesis of glycol chitin, a substrate of lysozyme. Carbohydrate Research 92, 160-162.