Purification and properties of a chitinase from Penicillium sp. LYG 0704

Protein Expression and Purification

Volumn 65, Issue 2, 2009, Pages 244-250

  Lee, Yoon Gyo ^a   Chung, Ki Chul ^a   Wi, Seung Gon ^a   Lee, Jae Chang ^b   Bae, Hyeun Jong ^a  

^a Chonnam National University   (South Korea)

^b Naju College   (South Korea)

Author keywords

Chitinase; Gene cloning; Penicillium sp.; Purification; RACE PCR

Indexed keywords

2 PROPANOL; CHITINASE; HYBRID PROTEIN;

ARTICLE; CHEMISTRY; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; PENICILLIUM; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

2-PROPANOL; CHITINASE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; PENICILLIUM; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

ASPERGILLUS FUMIGATUS; ASPERGILLUS FUMIGATUS AF293; PENICILLIUM; PENICILLIUM SP.;

EID: 61749102862     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.12.004     Document Type: Article

References (29)

1. Gooday G.W. Physiology of microbiol degradation of chitin and chitosan. Biodegradation 1 (1994) 177-190
2. Li X., Wang L.X., Wang X., and Roseman S. The chitin catabolic cascade in the marine bacterium Vibrio cholerae: characterization of a unique chitin oligosaccharide deacetylase. Glycobiology 17 (2007) 1377-1387
3. Shiro M., Ueda M., Kawaguchi T., and Arai M. Cloning of a cluster of chitinase genes from Aeromonas sp. no. 10S-24. Biochim. Biophys. Acta 1305 (1996) 44-48
4. Rupley J.A. The hydrolysis of chitin by concentrated hydrochloric acid, and the preparation of low-molecular-weight substrates for lysozyme. Biochim. Biophys. Acta 83 (1964) 245-255
5. Muraki E., Hiraga K., Oda K., and Aiba S. Production of N-acetyl-d-glucosamine from α-chitin by crude enzymes from Areomonas hydrophila H-2330. Carbohydr. Res. 337 (2002) 761-763
6. Il'ina A.V., Zueva O.Y., Lopatin S.A., and Varlamov V.P. Enzymatic hydrolysis of α-chitin. Prikl. Biokhim. Mikrobiol. 35 (2004) 1154-1156
7. Tanaka T., Fukui T., and Imanaka T. Different cleavage specificities of the dual catalytic domains in chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J. Biol. Chem. 276 (2001) 35629-35635
8. Henrissat B. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280 (1991) 309-316
9. Ohno T., Armand S., Hata T., Mikaidou N., Henrissat B., Mitsutomi M., and Watanabe T. A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037. J. Bacteriol. 178 (1996) 5065-5070
10. Perrakis A., Tews I., Dauter Z., Oppenheim A.B., Chet I., Wilson K.S., and Vorgisa C.E. Crystal structures of a bacterial chitinase at 2.3 Å resolution. Structure 2 (1994) 1169-1180
11. Terwisscha van Scheltinga A.C., Kalk K.H., Beintema J.J., and Dijkstra B.W. Crystal structures of hevamine, a plant defense protein with chitinase and lysozyme activity, and its complex with an inhibitor. Structure 2 (1994) 1181-1189
12. Smit E., Leeflang P., Glandorf B., Van Elsas J.D., and Wernars K. Analysis of fungal diversity in the wheat rhizosphere by sequencing of cloned PCR-amplified genes encoding 18S rRNA and temperature gradient gel electrophoresis. Appl. Environ. Microbiol. 65 (1999) 2614-2621
13. Fujita S.-I., Senda Y., Nakaguchi S., and Hashimoto T. Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains. J. Clin. Microbiol. 39 (2001) 3617-3622
14. Hsu S.C., and Lockwood J.L. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl. Microbiol. 29 (1975) 422-426
15. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31 (1959) 426-428
16. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
17. de la Cruz J., Hidalgo-Gallego A., Lora J.M., Benitez T., Pintor-Toro J.A., and Llobell A. Isolation and characterization of three chitinases from Trichoderma harzianum. Eur. J. Biochem. 206 (1992) 859-867
18. Kunz C., Sellam O., and Bertheau Y. Purification and characterization of a chitinase from the hyperparasitic fungus Aphanocladium album. Physiol. Mol. Plant Pathol. 40 (1992) 117-131
19. Ulhoa C.J., and Peberdy J.F. Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enzyme Microb. Technol. 14 (1992) 236-241
20. di Pietro A., Lorito M., Hayes C.K., Broadway R.M., and Harman G.E. Endochitinase from Gliocladium virens: isolation, characterization, and synergistic antifungal activity in combination with gliotoxin. Phytopathology 83 (1993) 308-313
21. Harman G.E., Hayes C.K., Lorito M., Broadway R.M., Di Pietro A., Peterbauer C., and Tronsmo A. Chitinolytic enzymes of Trichoderma harzianum: purification of chitobiosidase and endochitinase. Phytopathology 83 (1993) 313-318
22. Li D.-C., Zhang S.-H., Liu K.-Q., and Lu J. Purification and partial characterization of a chitinase from the mycoparasitic fungus Trichothecium roseum. J. Gen. Appl. Microbiol. 50 (2004) 35-39
23. Li D.-C., Chen S., and Lu J. Purification and partial characterization of two chitinase from the mycoparasitic fungus Talaromyces flavus. Mycopathologia 159 (2005) 223-229
24. McCormack J., Hackett T.J., Tuohy M.G., and Coughlan M.P. Chitinase production by Talaromyces emersonii. Biotechnol. Lett. 13 (1991) 677-682
25. Deane E.E., Whipps J.M., Lynch J.M., and Peberdy J.F. The purification and characterization of a Trichoderma harzianum exochitinase. Biochim. Biophys. Acta 1383 (1998) 101-110
26. Sakurada M., Morgavi D.P., Komatani K., Tomita T., and Onodera R. Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1. FEMS Microbiol. Lett. 137 (1996) 75-78
27. di Giambattista R., Federici F., Petruccioli M., and Fenice M. The chitinolytic activity of Penicillium janthinellum P9: purification, partial characterization and potential applications. J. Appl. Microbiol. 91 (2001) 498-505
28. Sahai A.S., and Manocha M.S. Chitinases of fungi and plants: their involvement in morphogenesis and host-parasite interaction. FEMS Microbio. Rev. 11 (1993) 317-338
29. Gaboriaud C., Bissery V., Benchetrit T., and Mornon J.P. Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences. FEBS Lett. 224 (1987) 149-155