Identification, characterization, and application of a virulence factor (EfEstA) from Enterococcus faecalis

Bioresource Technology

Volumn 143, Issue , 2013, Pages 691-694

  Jang, Eunjin ^a   Ryu, Bum Han ^a   Ju, Hansol ^a   Doohun Kim, T ^a,b  

^a Ajou University   (South Korea)

^b Tufts University   (United States)

Author keywords

CLEAs; EfEstA; Virulence factor

Indexed keywords

BACILLI; INDUSTRIAL APPLICATIONS;

CLEAS; CROSS-LINKED ENZYME AGGREGATES; EFESTA; ENTEROCOCCUS FAECALIS; ENZYME ASSAYS; INDUSTRIAL ENZYMES; PATHOGENIC BACTERIUM; VIRULENCE FACTORS;

ENZYMES;

EFESTA; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

BACTERIUM; ENZYME ACTIVITY; PATHOGEN; RECYCLING; VIRULENCE;

ARTICLE; ENTEROCOCCUS FAECALIS; ENZYME ASSAY; MOLECULAR MODEL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; RECYCLING;

ENTEROCOCCUS FAECALIS;

EID: 84880583555     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2013.06.086     Document Type: Article

References (16)

1. Bae S.Y., Ryu B.H., Jang E., Kim S., Kim T.D. Characterization and immobilization of a novel SGNH hydrolase (Est24) from Sinorhizobium meliloti. Appl. Microbiol. Biotechnol. 2013, 97:1637-1647.
2. Fisher K., Phillips C. The ecology, epidemiology and virulence of Enterococcus. Microbiology 2009, 155:1749-1757.
3. Gupta P., Dutt K., Misra S., Raghuwanshi S., Saxena R.K. Characterization of cross-linked immobilized lipase from thermophilic mould Thermomyces lanuginosa using glutaraldehyde. Bioresour. Technol. 2009, 100:4074-4076.
4. Hava D.L., Camilli A. Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol. Microbiol. 2002, 45:1389-1406.
5. Hwang H., Kim S., Yoon S., Ryu Y., Lee S.Y., Kim T.D. Characterization of a novel oligomeric SGNH-arylesterase from Sinorhizobium meliloti 1021. Int. J. Biol. Macromol. 2010, 46:145-152.
6. Ju H., Jang E., Ryu B.H., Kim T.D. Characterization and preparation of highly stable aggregates of a novel type of hydrolase (BL28) from Bacillus licheniformis. Bioresour. Technol. 2013, 128:81-86.
7. Kim M.H., Kang B.S., Kim S., Kim K.J., Lee C.H., Oh B.C., Park S.C., Oh T.K. The crystal structure of the estA protein, a virulence factor from Streptococcus pneumoniae. Proteins 2008, 70:578-583.
8. Kim S., Bae S.Y., Kim S.J., Ngo T.D., Kim K.K., Kim T.D. Characterization, amyloid formation, and immobilization of a novel SGNH hydrolase from Listeria innocua 11262. Int. J. Biol. Macromol. 2012, 50:103-111.
9. Kovacs-Simon A., Titball R.W., Michell S.L. Lipoproteins of bacterial pathogens. Infect. Immun. 2011, 79:548-561.
10. Kourist R., Jochens H., Bartsch S., Kuipers R., Padhi S.K., Gall M., Böttcher D., Joosten H.J., Bornscheuer U.T. The alpha/beta-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering. ChemBioChem 2010, 11:1635-1643.
11. Lun S., Bishai W.R. Characterization of a novel cell wall-anchored protein with carboxylesterase activity required for virulence in Mycobacterium tuberculosis. J. Biol. Chem. 2007, 282:18348-18356.
12. Ollis D.L., Cheah E., Cygler M., Dijkstra B., Frolow F., Franken S.M., Harel M., Remington S.J., Silman I., Schrag J., Sussman J.L., Verschueren K.G.H., Goldman A. The alpha/beta hydrolase fold. Protein Eng. 1992, 5:197-211.
13. Sava I.G., Heikens E., Huebner J. Pathogenesis and immunity in enterococcal infections. Clin. Microbiol. Infect. 2010, 16:533-540.
14. Schwede T., Kopp J., Guex N., Peitsch M.C. SWISS-MODEL: An automated protein homology-modeling server. Nucleic Acids Res. 2003, 31:3381-3385.
15. Sheldon R.A. Cross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts. Biochem. Soc. Trans. 2007, 35:1583-1587.
16. Willems R.J., Hanage W.P., Bessen D.E., Feil E.J. Population biology of gram-positive pathogens: high-risk clones for dissemination of antibiotic resistance. FEMS Microbiol. Rev. 2011, 35:872-900.