An assay for α 1,6-Fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection

Methods in Molecular Biology

Volumn 1022, Issue , 2013, Pages 335-348

  Ihara, Hideyuki ^a   Tsukamoto, Hiroki ^a   Taniguchi, Naoyuki ^b   Ikeda, Yoshitaka ^a  

^a SAGA UNIVERSITY   (Japan)

^b RIKEN   (Japan)

Author keywords

2 Aminopyridine; Asn linked oligosaccharide; Core; Fucose; Fucosyltransferase; FUT8; Glycoprotein; Glycosylation; N glycan; Pyridylamine

Indexed keywords

ALPHA 1,6 FUCOSYLTRANSFERASE; ASPARAGINE DERIVATIVE; FUCOSYLTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; DISSOLUTION; EGG YOLK; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ISOLATION; ENZYME MECHANISM; ENZYME PURIFICATION; FLUORESCENCE; FUCOSYLATION; GEL FILTRATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 84880485013     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-465-4_25     Document Type: Article

References (26)

1. Ihara H, Ikeda Y, Taniguchi N (2006) Reaction mechanism and substrate specifi city for nucleotide sugar of mammalian alpha1,6-fucosyltransferase-A large-Scale preparation and characterization of recombinant human FUT8. Glycobiology 16:333-342
2. Ihara H, Ikeda Y, Toma S, Wang X, Suzuki T, Gu J, Miyoshi E, Tsukihara T, Honke K, Matsumoto A, Nakagawa A, Taniguchi N (2007) Crystal structure of mammalian alpha1,6-fucosyltransferase, FUT8. Glycobiology 17:455-466
3. Ihara H, Hanashima S, Okada T, Ito R, Yamaguchi Y, Taniguchi N, Ikeda Y (2010) Fucosylation of chitooligosaccharides by human alpha1,6- fucosyltransferase requires a nonreducing terminal chitotriose unit as a minimal structure. Glycobiology 20:1021-1033
4. Wang X, Inoue S, Gu J, Miyoshi E, Noda K, Li W, Mizuno-Horikawa Y, Nakano M, Asahi M, Takahashi M, Uozumi N, Ihara S, Lee SH, Ikeda Y, Yamaguchi Y, Aze Y, Tomiyama Y, Fujii J, Suzuki K, Kondo A, Shapiro SD, Lopez-Otin C, Kuwaki T, Okabe M, Honke K, Taniguchi N (2005) Dysregulation of TGFbeta1 receptor activation leads to abnormal lung development and emphysema-Like phenotype in core fucose-defi cient mice. Proc Natl Acad Sci USA 102:15791-15796
5. Wang X, Gu J, Ihara H, Miyoshi E, Honke K, Taniguchi N (2006) Core fucosylation regulates epidermal growth factor receptormediated intracellular signaling. J Biol Chem 281:2572-2577
6. Wang X, Fukuda T, Li W, Gao CX, Kondo A, Matsumoto A, Miyoshi E, Taniguchi N, Gu J (2009) Requirement of Fut8 for the expression of vascular endothelial growth factor receptor-2: A new mechanism for the emphysema-Like changes observed in Fut8-defi cient mice. J Biochem 145:643-651
7. Lee SH, Takahashi M, Honke K, Miyoshi E, Osumi D, Sakiyama H, Ekuni A, Wang X, Inoue S, Gu J, Kadomatsu K, Taniguchi N (2006) Loss of core fucosylation of low-density lipoprotein receptor-related protein-1 impairs its function, leading to the upregulation of serum levels of insulin-Like growth factor-binding protein 3 in Fut8-/- mice. J Biochem 139:391-398
8. Osumi D, Takahashi M, Miyoshi E, Yokoe S, Lee SH, Noda K, Nakamori S, Gu J, Ikeda Y, Kuroki Y, Sengoku K, Ishikawa M, Taniguchi N (2009) Core fucosylation of E-cadherin enhances cell-cell adhesion in human colon carcinoma WiDr cells. Cancer Sci 100:888-895
9. Zhao Y, Itoh S, Wang X, Isaji T, Miyoshi E, Kariya Y, Miyazaki K, Kawasaki N, Taniguchi N, Gu J (2006) Deletion of core fucosylation on alpha3beta1 integrin down-regulates its functions. J Biol Chem 281:38343-38350
10. Li W, Ishihara K, Yokota T, Nakagawa T, Koyama N, Jin J, Mizuno-Horikawa Y, Wang X, Miyoshi E, Taniguchi N, Kondo A (2008) Reduced alpha4beta1 integrin/VCAM-1 interactions lead to impaired pre-B cell repopulation in alpha 1,6-fucosyltransferase defi cient mice. Glycobiology 18:114-124
11. Shields RL, Lai J, Keck R, O'Connell LY, Hong K, Meng YG, Weikert SH, Presta LG (2002) Lack of fucose on human IgG1 N-Linked oligosaccharide improves binding to human Fcgamma RIII and antibodydependent cellular toxicity. J Biol Chem 277:26733-26740
12. Shinkawa T, Nakamura K, Yamane N, Shoji-Hosaka E, Kanda Y, Sakurada M, Uchida K, Anazawa H, Satoh M, Yamasaki M, Hanai N, Shitara K (2003) The absence of fucose but not the presence of galactose or bisecting N-Acetylglucosamine of human IgG1 complextype oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity. J Biol Chem 278:3466-3473
13. Alpert ME, Uriel J, de Nechaud B (1968) Alpha-1 fetoglobulin in the diagnosis of human hepatoma. N Engl J Med 278:984-986
14. Ruoslahti E, Salaspuro M, Pihko H, Andersson L, Seppala M (1974) Serum alpha-fetoprotein: Diagnostic signifi cance in liver disease. Br Med J 2:527-529
15. Taketa K (1990) Alpha-fetoprotein: Reevaluation in hepatology. Hepatology 12:1420-1432
16. Aoyagi Y (1995) Carbohydrate-based measurements on alpha-fetoprotein in the early diagnosis of hepatocellular carcinoma. Glycoconj J 12:194-199
17. Miyoshi E, Noda K, Yamaguchi Y, Inoue S, Ikeda Y, Wang W, Ko JH, Uozumi N, Li W, Taniguchi N (1999) The alpha1-6-fucosyltransferase gene and its biological signifi cance. Biochim Biophys Acta 1473:9-20
18. Takahashi T, Ikeda Y, Miyoshi E, Yaginuma Y, Ishikawa M, Taniguchi N (2000) alpha1,6fucosyltransferase is highly and specifically expressed in human ovarian serous adenocarcinomas. Int J Cancer 88:914-919
19. Nishikawa A, Fujii S, Sugiyama T, Taniguchi N (1988) A method for the determination of N-Acetylglucosaminyltransferase III activity in rat tissues involving HPLC. Anal Biochem 170:349-354
20. Tomiya N, Lee YC, Yoshida T, Wada Y, Awaya J, Kurono M, Takahashi N (1991) Calculated two-dimensional sugar map of pyridylaminated oligosaccharides: Elucidation of the jack bean alpha-mannosidase digestion pathway of Man9GlcNAc2. Anal Biochem 193:90-100
21. Kondo A, Suzuki J, Kuraya N, Hase S, Kato I, Ikenaka T (1990) Improved method for fl uorescence labeling of sugar chains with sialic acid residues. Agric Biol Chem 54:2169-2170
22. Palmerini CA, Datti A, Alunni S, VanderElst IE, Orlacchio A (1995) A fl uorescent assay for the determination of UDP-GlcNAc: Gal beta 1,3GalNAc-R (GlcNAc to GalNAc) beta 1,6-N-Acetylglucosaminyltransferase activity. Anal Biochem 225:315-320
23. Sasaki K, Kurata K, Funayama K, Nagata M, Watanabe E, Ohta S, Hanai N, Nishi T (1994) Expression cloning of a novel alpha 1,3-fucosyltransferase that is involved in biosynthesis of the sialyl Lewis × carbohydrate determinants in leukocytes. J Biol Chem 269:14730-14737
24. Mita Y, Aoyagi Y, Suda T, Asakura H (2000) Plasma fucosyltransferase activity in patients with hepatocellular carcinoma, with special reference to correlation with fucosylated species of alpha-fetoprotein. J Hepatol 32:946-954
25. Uozumi N, Teshima T, Yamamoto T, Nishikawa A, Gao YE, Miyoshi E, Gao CX, Noda K, Islam KN, Ihara Y, Fujii S, Shiba T, Taniguchi N (1996) A fl uorescent assay method for GDP-L-Fuc:N-Acetyl-beta-Dglucosaminide alpha 1-6fucosyltransferase activity, involving high performance liquid chromatography. J Biochem 120:385-392
26. Seko A, Koketsu M, Nishizono M, Enoki Y, Ibrahim HR, Juneja LR, Kim M, Yamamoto T (1997) Occurrence of a sialylglycopeptide and free sialylglycans in hen's egg yolk. Biochim Biophys Acta 1335:23-32