Bacillus subtilis alkaline phosphatase IV acquires activity only late at the stationary phase when produced in Escherichia coli. Overexpression and characterization of the recombinant enzyme

Protein Expression and Purification

Volumn 90, Issue 2, 2013, Pages 186-194

  Koksharov, Mikhail ^a,b   Lv, Changqing ^a   Zhai, Xiaohong ^a   Ugarova, Natalia ^b   Huang, Eric ^a  

^a HANGZHOU NORMAL UNIVERSITY   (China)

^b MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

Alkaline phosphatase; Bacillus subtilis; Expression in Escherichia coli; Protein folding; Solubility; Stationary phase

Indexed keywords

ALKALINE PHOSPHATASE; BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS SUBTILIS; CHEMISTRY; CYTOPLASM; ENZYMOLOGY; ESCHERICHIA COLI; EXPRESSION IN ESCHERICHIA COLI; GENETICS; METABOLISM; PH; PROTEIN FOLDING; SOLUBILITY; STATIONARY PHASE; TEMPERATURE;

ALKALINE PHOSPHATASE; BACILLUS SUBTILIS; EXPRESSION IN ESCHERICHIA COLI; PROTEIN FOLDING; SOLUBILITY; STATIONARY PHASE;

ALKALINE PHOSPHATASE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; PERIPLASM; RECOMBINANT PROTEINS; TEMPERATURE;

EID: 84880302727     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.06.008     Document Type: Article

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