Cloning of an alkaline phosphatase gene from the moderately thermophilic bacterium Meiothermus ruber and characterization of the recombinant enzyme

Molecular Genetics and Genomics

Volumn 270, Issue 1, 2003, Pages 87-93

  Yurchenko, J V ^a   Budilov, A V ^b   Deyev, S M ^b,c   Khromov, I S ^a   Sobolev, A Y ^a  

^a INSTITUTE OF MOLECULAR GENETICS   (Russian Federation)

^b ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

^c SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

Author keywords

Alkaline phosphatase; Heterologous gene expression; Meiothermus ruber

Indexed keywords

4 NITROPHENYL PHOSPHATE; ALKALINE PHOSPHATASE; MAGNESIUM ION; RECOMBINANT DNA; RECOMBINANT ENZYME; SIGNAL PEPTIDE; ZINC ION;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; BINDING SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; GENE CONSTRUCT; GENE SEQUENCE; GENETIC CONSERVATION; INCUBATION TIME; MICHAELIS CONSTANT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRECURSOR; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; TEMPERATURE; THERMOPHILIC BACTERIUM;

ALKALINE PHOSPHATASE; AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CLONING, MOLECULAR; DEINOCOCCUS; DNA PRIMERS; ESCHERICHIA COLI; KINETICS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MEIOTHERMUS; MEIOTHERMUS RUBER;

EID: 0142044009     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-003-0899-y     Document Type: Article

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