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Volumn 151, Issue 1, 2008, Pages 81-92
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A moderately thermostable alkaline phosphatase from Geobacillus thermodenitrificans T2: Cloning, expression and biochemical characterization
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Author keywords
Alkaline phosphatase; Characterization; Cloning; Geobacillus; Thermostable
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Indexed keywords
ACTIVE SITES;
ALKALINE PHOSPHATASE;
BIOCHEMICAL CHARACTERIZATIONS;
DITHIOTHREITOL;
DIVALENT CATIONS;
E. COLI;
ETHYLENE DIAMINE TETRA ACETIC ACIDS;
FUSION PROTEINS;
GEOBACILLUS;
GEOBACILLUS THERMODENITRIFICANS;
HIGH ACTIVITIES;
MOLECULAR CLONINGS;
OPTIMAL CONDITIONS;
PURIFIED ENZYMES;
SIGNAL PEPTIDES;
STRUCTURE ELEMENTS;
THERMOSTABLE;
AMINATION;
AMINES;
AMINO ACIDS;
AMPLIFICATION;
ENZYME ACTIVITY;
ENZYMES;
ESCHERICHIA COLI;
GENE ENCODING;
ORGANIC ACIDS;
PURIFICATION;
UREA;
ZINC;
CLONING;
ALKALINE PHOSPHATASE;
BETANIDINE;
DITHIOTHREITOL;
UREA;
RECOMBINANT PROTEIN;
ARTICLE;
CONTROLLED STUDY;
ENZYME ACTIVITY;
ENZYME KINETICS;
ENZYME PURIFICATION;
ENZYME STRUCTURE;
GENE OVEREXPRESSION;
GENE SEQUENCE;
GEOBACILLUS;
GEOBACILLUS THERMODENITRIFICANS;
MOLECULAR CLONING;
NONHUMAN;
PROTEIN EXPRESSION;
SEQUENCE HOMOLOGY;
THERMOSTABILITY;
CHEMISTRY;
ENZYME ACTIVATION;
ENZYME STABILITY;
ENZYMOLOGY;
ESCHERICHIA COLI;
GENETICS;
GEOBACTER;
METABOLISM;
METHODOLOGY;
PROTEIN ENGINEERING;
TEMPERATURE;
ESCHERICHIA COLI;
GEOBACILLUS;
GEOBACILLUS THERMODENITRIFICANS;
ALKALINE PHOSPHATASE;
CLONING, MOLECULAR;
ENZYME ACTIVATION;
ENZYME STABILITY;
ESCHERICHIA COLI;
GEOBACTER;
PROTEIN ENGINEERING;
RECOMBINANT PROTEINS;
TEMPERATURE;
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EID: 53749100080
PISSN: 02732289
EISSN: None
Source Type: Journal
DOI: 10.1007/s12010-008-8166-7 Document Type: Article |
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Times cited : (12)
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References (43)
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