Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds

Methods in Molecular Biology

Volumn 1030, Issue , 2013, Pages 283-299

  Alcaraz Estrada, Sofia L ^a   Reichert, Erin Donohue ^b   Padmanabhan, Radhakrishnan ^c  

^a INSTITUTO MEXICANO DEL SEGURO SOCIAL   (Mexico)

^b DEFENSE THREAT REDUCTION AGENCY   (United States)

^c GEORGETOWN UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bicistronic expression; Foot and mouth disease virus 2A; Neomycin resistance selection; Overlap extension PCR; Renilla luciferase reporter; Replicon construction; West Nile virus

Indexed keywords

ANTIBIOTIC G 418; ANTIVIRUS AGENT; KANAMYCIN KINASE; NONSTRUCTURAL PROTEIN 5; RECOMBINANT DNA; RENILLA LUCIFERIN 2 MONOOXYGENASE;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; BACTERIUM TRANSFORMATION; CARBOXY TERMINAL SEQUENCE; CELL CLONING; CONTROLLED STUDY; DRUG SCREENING; ELECTROPORATION; IN VITRO STUDY; INTERNAL RIBOSOME ENTRY SITE; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPLICON; REPORTER GENE; VERO CELL; VIRUS GENE; VIRUS REPLICATION; WEST NILE FLAVIVIRUS;

ANIMALS; ANTIVIRAL AGENTS; CELL LINE; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; GENE EXPRESSION; GENE ORDER; GENES, REPORTER; GENETIC VECTORS; GENOME, VIRAL; MICROBIAL SENSITIVITY TESTS; TRANSFECTION; VERO CELLS; VIRUS REPLICATION; WEST NILE VIRUS;

EID: 84880118091     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-484-5_22     Document Type: Article

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