High-throughput screening using dengue virus reporter genomes

Methods in Molecular Biology

Volumn 1030, Issue , 2013, Pages 205-219

  Fischl, Wolfgang ^a   Bartenschlager, Ralf ^b  

^a Haplogen GmbH   (Austria)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Dengue virus; High throughput screening; Luciferase; Reporter virus; RNA interference

Indexed keywords

COMPLEMENTARY DNA; LUCIFERASE; SMALL INTERFERING RNA; VIRUS RNA;

ANIMAL CELL; ARTICLE; CULTURE MEDIUM; DENGUE VIRUS; ENZYME ASSAY; GENOME ANALYSIS; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPORTER GENE; RNA TRANSCRIPTION; VIRUS CULTURE; VIRUS GENOME;

ANIMALS; ANTIVIRAL AGENTS; CELL CULTURE TECHNIQUES; CELL LINE; CELL-FREE SYSTEM; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; CRICETINAE; DENGUE VIRUS; DNA, COMPLEMENTARY; GENES, REPORTER; GENOME, VIRAL; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROBIAL SENSITIVITY TESTS; TRANSCRIPTION, GENETIC; VERO CELLS; VIRUS REPLICATION;

EID: 84880113515     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-484-5_17     Document Type: Article

References (9)

1. Guzman MG, Halstead SB, Artsob H et al (2010) Dengue: a continuing global threat. Nat Rev Microbiol 8:S7-S16
2. Kinney RM, Butrapet S, Chang GJ et al (1997) Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53. Virology 230:300-308
3. Lohmann V, Korner F, Koch J, Herian U, Theilmann L, Bartenschlager R (1999) Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line. Science 285:110-113
4. Donnelly ML, Hughes LE, Luke G et al (2001) The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences. J Gen Virol 82:1027-1041
5. Alvarez DE, Lodeiro MF, Luduena SJ et al (2005) Long-range RNA-RNA interactions circularize the dengue virus genome. J Virol 79:6631-6643
6. Erfl e H, Neumann B, Rogers P et al (2008) Work fl ow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates. J Biomol Screen 13:575-580
7. Kärber G (1931) Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch Exp Pathol Pharmakol 162: 480-487
8. Spearman C (1908) The method of right and wrong cases (constant stimuli) without Gauss's formulae. Br J Psychol 2:227-242
9. Lindenbach BD, Evans MJ, Syder AJ et al (2005) Complete replication of hepatitis C virus in cell culture. Science 309:623-666