Degenerative grade affects the responses of human nucleus pulposus cells to Link-N, CTGF, and TGFβ3

Journal of Spinal Disorders and Techniques

Volumn 26, Issue 3, 2013, Pages

  Abbott, Rosalyn D ^a   Purmessur, Devina ^b   Monsey, Robert D ^c   Brigstock, David R ^d   Laudier, Damien M ^b   Iatridis, James C ^b  

^a University of Vermont   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF VERMONT   (United States)

^d THE RESEARCH INSTITUTE AT NATIONWIDE CHILDREN S HOSPITAL   (United States)

Author keywords

CTGF; Degeneration; Link N; Nucleus pulposus; TGF

Indexed keywords

AGGRECANASE 2; ALGINIC ACID; CELL DNA; COL1A1 PROTEIN; COLLAGEN TYPE 1; CONNECTIVE TISSUE GROWTH FACTOR; DEXAMETHASONE; GELATINASE B; GLYCOSAMINOGLYCAN; INTERLEUKIN 1BETA; LINK PROTEIN; TISSUE SCAFFOLD; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 1; TRANSFORMING GROWTH FACTOR BETA3; UNCLASSIFIED DRUG;

ADULT; ARTICLE; BIOSYNTHESIS; BONE CELL; CATABOLISM; CELL ASSAY; CELL ISOLATION; CELL LEVEL; CELL METABOLISM; CELL PROLIFERATION; CELL REGENERATION; CELL STIMULATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA CONTENT; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERVERTEBRAL DISK DEGENERATION; MALE; NUCLEUS PULPOSUS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION;

ADULT; CELL PROLIFERATION; CELLS, CULTURED; COLLAGEN TYPE I; CONNECTIVE TISSUE GROWTH FACTOR; DEXAMETHASONE; FEMALE; HUMANS; INTERLEUKIN-1BETA; INTERVERTEBRAL DISC; INTERVERTEBRAL DISC DEGENERATION; MALE; MATRIX METALLOPROTEINASE 9; MIDDLE AGED; PROTEIN-SERINE-THREONINE KINASES; RECEPTORS, TRANSFORMING GROWTH FACTOR BETA; SEVERITY OF ILLNESS INDEX; TRANSFORMING GROWTH FACTOR BETA3;

EID: 84880046620     PISSN: 15360652     EISSN: 15392465     Source Type: Journal    
DOI: 10.1097/BSD.0b013e31826e0ca4     Document Type: Article

References (37)

1. Masuda K, An HS. Prevention of disc degeneration with growth factors. Eur Spine J. 2006;15(suppl 3):S422-S432.
2. Aoyama E, Hattori T, Hoshijima M, et al. N-terminal domains of CCN family 2/connective tissue growth factor bind to aggrecan. Biochem J. 2009;420:413-420.
3. Petit A, Yao G, Rowas SA, et al. Effect of synthetic Link N peptide on the expression of type I and type II collagens in human intervertebral disc cells. Tissue Eng Part A. 2011;17:899-904.
4. Mwale F, Demers CN, Petit A, et al. A synthetic peptide of link protein stimulates the biosynthesis of collagens II, IX and proteoglycan by cells of the intervertebral disc. J Cell Biochem. 2003;88:1202-1213.
5. Liu H, McKenna LA, Dean MF. An N-terminal peptide from link protein can stimulate biosynthesis of collagen by human articular cartilage. Arch Biochem Biophys. 2000;378:116-122.
6. Liu Y, Kong J, Chen BH, et al. Combined expression of CTGF and tissue inhibitor of metalloprotease-1 promotes synthesis of proteoglycan and collagen type II in rhesus monkey lumbar intervertebral disc cells in vitro. Chin Med J (Engl). 2010;123:2082-2087.
7. Neame PJ, Barry FP. The link proteins. Experientia. 1993;49: 393-402.
8. Rodriguez E, Roughley P. Link protein can retard the degradation of hyaluronan in proteoglycan aggregates. Osteoarthritis Cartilage. 2006;14:823-829.
9. Mwale F, Masuda K, Pichika R, et al. The efficacy of Link N as a mediator of repair in a rabbit model of intervertebral disc degeneration. Arthritis Res Ther. 2011;13:R120.
10. Erwin WM, Ashman K, O'Donnel P, et al. Nucleus pulposus notochord cells secrete connective tissue growth factor and upregulate proteoglycan expression by intervertebral disc chondrocytes. Arthritis Rheum. 2006;54:3859-3867.
11. Tran CM, Markova D, Smith HE, et al. Regulation of CCN2/connective tissue growth factor expression in the nucleus pulposus of the intervertebral disc: role of Smad and activator protein 1 signaling. Arthritis Rheum. 2010;62:1983-1992.
12. Pelton RW, Dickinson ME, Moses HL, et al. In situ hybridization analysis of TGF beta 3 RNA expression during mouse development: comparative studies with TGF beta 1 and beta 2. Development. 1990;110:609-620.
13. Hiyama A, Gogate SS, Gajghate S, et al. BMP-2 and TGF-beta stimulate expression of beta1,3-glucuronosyl transferase 1 (GlcAT-1) in nucleus pulposus cells through AP1, TonEBP, and Sp1: role of MAPKs. J Bone Miner Res. 2010;25:1179-1190.
14. Reza AT, SB Nicoll. Serum-free, chemically defined medium with TGF-beta(3) enhances functional properties of nucleus pulposus cell-laden carboxymethylcellulose hydrogel constructs. Biotechnol Bioeng. 2010;105:384-395.
15. Jee BK, Surendran S, Park KM, et al. Role of tumor necrosis factoralpha, interleukin-8, and dexamethasone in the focal adhesion kinase expression by human nucleus pulposus cells. Spine (Phila Pa 1976). 2007;32:30-35.
16. Boeuf S, Richter W. Chondrogenesis of mesenchymal stem cells: role of tissue source and inducing factors. Stem Cell Res Ther. 2010;1:31.
17. Steck E, Bertram H, Abel R, et al. Induction of intervertebral disclike cells from adult mesenchymal stem cells. Stem Cells. 2005;23: 403-411.
18. Abbott RD, Purmessur D, Monsey RD, et al. Regenerative potential of TGFbeta3+Dex and notochordal cell conditioned media on degenerated human intervertebral disc cells. J Orthop Res. 2011.
19. Le Maitre CL, Freemont AJ, Hoyland JA. Localization of degradative enzymes and their inhibitors in the degenerate human intervertebral disc. J Pathol. 2004;204:47-54.
20. Gruber HE, Ingram JA, Norton HJ, et al. Senescence in cells of the aging and degenerating intervertebral disc: immunolocalization of senescence- associated beta-galactosidase in human and sand rat discs. Spine (Phila Pa 1976). 2007;32:321-327.
21. Le Maitre CL, Freemont AJ, Hoyland JA. Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration. Arthritis Res Ther. 2007;9:R45.
22. Ball DK, Moussad EE, Rageh MA, et al. Establishment of a recombinant expression system for connective tissue growth factor (CTGF) that models CTGF processing in utero. Reproduction. 2003;125:271-284.
23. Chen J, Yan W, Setton LA. Static compression induces zonalspecific changes in gene expression for extracellular matrix and cytoskeletal proteins in intervertebral disc cells in vitro. Matrix Biol. 2004;22:573-583.
24. Lillie RD. Histopathologic Technic and Practical Histochemistry. New York, NY: McGraw-Hill Inc.; 1976.
25. Purmessur D, Schek RM, Abbott RD, et al. Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells. Arthritis Res Ther. 2011;13:R81.
26. Mwale F, Roughley P, Antoniou J. Distinction between the extracellular matrix of the nucleus pulposus and hyaline cartilage: a requisite for tissue engineering of intervertebral disc. Eur Cell Mater. 2004;8:58-63; discussion -4.
27. Gantenbein-Ritter B, Benneker LM, Alini M, et al. Differential response of human bone marrow stromal cells to either TGF-beta(1) or rhGDF-5. Eur Spine J. 2011;20:962-971.
28. Scott JE, Bosworth TR, Cribb AM, et al. The chemical morphology of age-related changes in human intervertebral disc glycosaminoglycans from cervical, thoracic and lumbar nucleus pulposus and annulus fibrosus. J Anat. 1994;184(pt 1):73-82.
29. Camplejohn KL, Allard SA. Limitations of safranin 'O' staining in proteoglycan-depleted cartilage demonstrated with monoclonal antibodies. Histochemistry. 1988;89:185-188.
30. Loreto C, Musumeci G, Castorina A, et al. Degenerative disc disease of herniated intervertebral discs is associated with extracellular matrix remodeling, vimentin-positive cells and cell death. Ann Anat. 2011;193:156-162.
31. Roberts S, Caterson B, Menage J, et al. Matrix metalloproteinases and aggrecanase: their role in disorders of the human intervertebral disc. Spine (Phila Pa 1976). 2000;25:3005-3013.
32. Le Maitre CL, Pockert A, Buttle DJ, et al. Matrix synthesis and degradation in human intervertebral disc degeneration. Biochem Soc Trans. 2007;35:652-655.
33. Rutges JP, Nikkels PG, Oner FC, et al. The presence of extracellular matrix degrading metalloproteinases during fetal development of the intervertebral disc. Eur Spine J. 2010;19:1340-1346.
34. Cs-Szabo G, Ragasa-San Juan D, Turumella V, et al. Changes in mRNA and protein levels of proteoglycans of the anulus fibrosus and nucleus pulposus during intervertebral disc degeneration. Spine (Phila Pa 1976). 2002;27:2212-2219.
35. Chen J, Yan W, Setton LA. Molecular phenotypes of notochordal cells purified from immature nucleus pulposus. Eur Spine J. 2006;15(suppl 3):S303-S311.
36. Blaney Davidson EN, Remst DF, Vitters EL, et al. Increase in ALK1/ALK5 ratio as a cause for elevated MMP-13 expression in osteoarthritis in humans and mice. J Immunol. 2009;182: 7937-7945.
37. Wuertz K, Vo N, Kletsas D, et al. Inflammatory and catabolic signalling in intervertebral discs: the roles of NF-kappaB and MAP kinases. Eur Cell Mater. 2012;23:103-119; discussion 19-20.