Impact of reference gene selection for type 2 cannabinoid receptor gene expression studies in human spermatozoa

Andrologia

Volumn 45, Issue 4, 2013, Pages 278-284

  Amoako, A A ^a   Gebeh, A K ^a   Marczylo, E L ^b   Willets, J M ^a   Elson, J ^c   Marczylo, T H ^a   Konje, J C ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b MRC TOXICOLOGY UNIT   (United Kingdom)

^c LEICESTER ROYAL INFIRMARY   (United Kingdom)

Author keywords

Gene expression; GeNorm; Human spermatozoa; NormFinder; Reference genes

Indexed keywords

BETA ACTIN; CANNABINOID 2 RECEPTOR; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; RNA 18S;

ARTICLE; GENE EXPRESSION PROFILING; HOUSEKEEPING GENE; HUMAN; MALE; NORMAL HUMAN; REAL TIME POLYMERASE CHAIN REACTION; RECEPTOR GENE; SPERM; SPERMATOZOON;

GENE EXPRESSION; GENORM; HUMAN SPERMATOZOA; NORMFINDER; REFERENCE GENES;

GENE EXPRESSION PROFILING; HUMANS; INFERTILITY, MALE; MALE; RECEPTOR, CANNABINOID, CB2; REFERENCE STANDARDS; RNA, MESSENGER; SPERMATOZOA;

EID: 84879875915     PISSN: 03034569     EISSN: 14390272     Source Type: Journal    
DOI: 10.1111/and.12006     Document Type: Article

References (23)

1. Agirregoitia E, Carracedo A, Subirán N, Valdivia A, Agirregoitia N, Peralta L, Velasco G, Irazusta J (2010) The CB(2) cannabinoid receptor regulates human sperm cell motility. Fertil Steril 93:1378-1387.
2. Aitken RJ, Wingate JK, De Iuliis GN, Koppers AJ, Mclaughlin EA (2006) Cis-unsaturated fatty acids stimulate reactive oxygen species generation and lipid peroxidation in human spermatozoa. J Clin Endocrinol Metab 91:4154-4163.
3. Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245-5250.
4. Aquila S, Guido C, Santoro A, Perrotta I, Laezza C, Bifulco M, Sebastiano A (2010) Human sperm anatomy: ultrastructural localization of the cannabinoid1 receptor and a potential role of anandamide in sperm survival and acrosome reaction. Anat Rec (Hoboken) 293:298-309.
5. Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-193.
6. Bustin SA (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 29:23-39.
7. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611-622.
8. Feng X, Xiong Y, Qian H, Lei M, Xu D, Ren Z (2010) Selection of reference genes for gene expression studies in porcine skeletal muscle using SYBR green qPCR. J Biotechnol 150:288-293.
9. Ginzinger DG (2002) Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 30:503-512.
10. Grimaldi P, Orlando P, Di Siena S, Lolicato F, Petrosino S, Bisogno T, Geremia R, De Petrocellis L, Di Marzo V (2009) The endocannabinoid system and pivotal role of the CB2 receptor in mouse spermatogenesis. Proc Natl Acad Sci USA 106:11131-11136.
11. Huggett J, Dheda K, Bustin S, Zumla A (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6:279-284.
12. Hull MG, Glazener CM, Kelly NJ, Conway DI, Foster PA, Hinton RA, Coulson C, Lambert PA, Watt EM, Desai KM (1985) Population study of causes, treatment, and outcome of infertility. Br Med J (Clin Res Ed) 291:1693-1697.
13. Jeffreys AJ, Neumann R, Wilson V (1990) Repeat unit sequence variation in minisatellites: a novel source of DNA polymorphism for studying variation and mutation by single molecule analysis. Cell 60:473-485.
14. Jeffreys AJ, Tamaki K, Macleod A, Monckton DG, Neil DL, Armour JA (1994) Complex gene conversion events in germline mutation at human minisatellites. Nat Genet 6:136-145.
15. Maccarrone M, Barboni B, Paradisi A, Bernabo N, Gasperi V, Pistilli MG, Fezza F, Lucidi P, Mattioli M (2005) Characterization of the endocannabinoid system in boar spermatozoa and implications for sperm capacitation and acrosome reaction. J Cell Sci 118:4393-4404.
16. Mehta R, Birerdinc A, Hossain N, Afendy A, Chandhoke V, Younossi Z, Baranova A (2010) Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples. BMC Mol Biol 11:39.
17. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45.
18. Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A (2004) Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun 313:856-862.
19. Romanowski T, Markiewicz A, Bednarz N, Bielawski KP (2007) [Housekeeping genes as a reference in quantitative real-time RT-PCR]. Postepy Hig Med Dosw (Online) 61:500-510.
20. Small BC, Murdock CA, Bilodeau-Bourgeois AL, Peterson BC, Waldbieser GC (2008) Stability of reference genes for real-time PCR analyses in channel catfish (Ictalurus punctatus) tissues under varying physiological conditions. Comp Biochem Physiol B Biochem Mol Biol 151:296-304.
21. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:RESEARCH0034.
22. Wan H, Zhao Z, Qian C, Sui Y, Malik AA, Chen J (2010) Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber. Anal Biochem 399:257-261.
23. World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen. 5th edn. WHO Press, Geneva, Switzerland.