An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli

PLoS ONE

Volumn 8, Issue 7, 2013, Pages

  Schiffels, Johannes ^a   Pinkenburg, Olaf ^b   Schelden, Maximilian ^a   Aboulnaga, El Hussiny A A ^a   Baumann, Marcus E M ^a   Selmer, Thorsten ^a  

^a AACHEN UNIVERSITY OF APPLIED SCIENCES   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGENASE; MESSENGER RNA; NICOTINAMIDE ADENINE DINUCLEOTIDE; PERMEASE; PROTEINASE;

ARTICLE; BACTERIAL GENE; BINDING AFFINITY; CATALYSIS; CELL DENSITY; CUPRIAVIDUS NECATOR; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE CLUSTER; GENE DELETION; GENE EXPRESSION SYSTEM; MOLECULAR CLONING; NONHUMAN; OPERON; PHOTOSYSTEM I; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN SYNTHESIS; ULTRAVIOLET SPECTROSCOPY;

BATCH CELL CULTURE TECHNIQUES; CLONING, MOLECULAR; CUPRIAVIDUS NECATOR; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION; GENE ORDER; GENETIC VECTORS; HYDROGENASE; PROTEIN MULTIMERIZATION; RECOMBINANT FUSION PROTEINS;

CUPRIAVIDUS NECATOR; ESCHERICHIA COLI;

EID: 84879802270     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0068812     Document Type: Article

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