Overview of electron crystallography of membrane proteins: Crystallization and screening strategies using negative stain electron microscopy

Current Protocols in Protein Science

Volumn , Issue SUPPL.72, 2013, Pages

  Nannenga, Brent L ^a   Iadanza, Matthew G ^a   Vollmar, Breanna S ^a   Gonen, Tamir ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Electron crystallography; Membrane proteins; Negative stain electron microscopy; Protein purification; Protein solubilization

Indexed keywords

ADENOSINE A2A RECEPTOR; DETERGENT; MEMBRANE PROTEIN; NEUROTENSIN RECEPTOR;

ANALYTICAL PARAMETERS; ARTICLE; CRYSTALLIZATION; CRYSTALLOGRAPHY; ELECTRON CRYSTALLOGRAPHY; ELECTRON MICROSCOPY; ENZYME ACTIVITY; ENZYME RECONSTITUTION; ENZYME SYNTHESIS; NEGATIVE STAIN ELECTRON MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SOLUBILIZATION; STAINING; TEMPERATURE; CHEMISTRY; CONFORMATION; CRYOELECTRON MICROSCOPY; METHODOLOGY; CRYSTAL STRUCTURE; HUMAN; LIPID BILAYER; PROTEIN ANALYSIS; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

CRYOELECTRON MICROSCOPY; CRYSTALLOGRAPHY; MEMBRANE PROTEINS; MOLECULAR CONFORMATION; NEGATIVE STAINING;

MLCS; MLOWN;

EID: 84879775726     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps1715s72     Document Type: Article

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