Analysis of mutants disrupted in bacillithiol metabolism in Staphylococcus aureus

Biochemical and Biophysical Research Communications

Volumn 436, Issue 2, 2013, Pages 128-133

  Rajkarnikar, Arishma ^a   Strankman, Andrew ^a   Duran, Shayla ^a   Vargas, Derek ^a   Roberts, Alexandra A ^b   Barretto, Kathryn ^a   Upton, Heather ^a   Hamilton, Christopher J ^b   Rawat, Mamta ^a  

^a CALIFORNIA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

Bacillithiol; Bacillithiol conjugate amidase; Bacillthiol S transferase; Bacillus subtilis; Staphylococcus aureus; Thiols

Indexed keywords

1 CHLORO 2,4 DINITROBENZENE; AMIDASE; BACILLITHIOL; BACTERIAL ENZYME; CERULENIN; DEACETYLASE; RIFAMYCIN; THIOL; TRANSFERASE; UNCLASSIFIED DRUG;

ALKYLATION; ARTICLE; BACTERIUM MUTANT; BIOSYNTHESIS; CONJUGATION; CONTROLLED STUDY; DETOXIFICATION; DRUG METABOLISM; GENE DISRUPTION; METAL STRESS; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; STRESS; TRANSPOSON;

BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; GLUCOSAMINE; IODOACETAMIDE; METALS; MICROBIAL VIABILITY; MUTATION; OXIDANTS; PYRUVALDEHYDE; STAPHYLOCOCCUS AUREUS; SULFHYDRYL COMPOUNDS; TIME FACTORS;

BACILLUS SUBTILIS; POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 84879606138     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.04.027     Document Type: Article

References (25)

1. Jothivasan V.K., Hamilton C.J. Mycothiol: synthesis, biosynthesis and biological functions of the major low molecular weight thiol in actinomycetes. Natural Products Reports 2008, 25:1091-1117.
2. Newton G.L., Buchmeier N., Fahey R.C. Biosynthesis and functions of mycothiol, the unique protective thiol of actinobacteria. Microbiology and Molecular Biology Reviews 2008, 72:471-494.
3. Rawat M., Av-Gay Y. Mycothiol-dependent proteins in actinomycetes. FEMS Microbiology Reviews 2007, 31:278-292.
4. Newton G.L., Rawat M., La Clair J.J., Jothivasan V.K., Budiarto T., Hamilton C.J., Claiborne A., Helmann J.D., Fahey R.C. Bacillithiol is an antioxidant thiol produced in Bacilli. Nature Chemical Biology 2009, 5:625-627.
5. Newton G.L., Fahey R.C., Rawat M. Detoxification of toxins by bacillithiol in Staphylococcus aureus. Microbiology 2012, 158:1117-1126.
6. Upton H., Newton G.L., Gushiken M., Lo K., Holden D., Fahey R.C., Rawat M. Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis. FEBS Letters 2012, 586:1004-1008.
7. Gaballa A., Newton G.L., Antelmann H., Parsonage D., Upton H., Rawat M., Claiborne A., Fahey R.C., Helmann J.D. Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli. Proceedings of the National Academy of Sciences 2010, 107:6482-6486.
8. Parsonage D., Newton G.L., Holder R.C., Wallace B.D., Paige C., Hamilton C.J., Dos Santos P.C., Redinbo M.R., Reid S.D., Claiborne A. Characterization of the N-acetyl-α-d-glucosaminyl l-malate synthase and deacetylase functions for bacillithiol biosynthesis in Bacillus anthracis. Biochemistry 2010, 49:8398-8414.
9. Masip L., Veeravalli K., Georgiu G. The many faces of glutathione in bacteria. Antioxidants & Redox Signaling 2006, 8:753-762.
10. Kanzok S.M., Fechner A., Bauer H., Ulschmid J.K., Müller H.-M., Botella-Munoz J., Schneuwly S., Schirmer R.H., Becker K. Substitution of the thioredoxin system for glutathione reductase in Drosophila melanogaster. Science 2001, 291:643-646.
11. Allocati N., Federici L., Masulli M., Di Ilio C. Glutathione transferases in bacteria. FEBS Journal 2009, 276:58-75.
12. Gold B., Pingle M., Brickner S.J., Shah N., Roberts J., Rundell M., Bracken W.C., Warrier T., Somersan S., Venugopal A., Darby C., Jiang X., Warren J.D., Fernandez J., Ouerfelli O., Nuermberger E.L., Cunningham-Bussel A., Rath P., Chidawanyika T., Deng H., Realubit R., Glickman J.F., Nathan C.F. Nonsteroidal anti-inflammatory drug sensitizes Mycobacterium tuberculosis to endogenous and exogenous antimicrobials. Proceedings of the National Academy of Sciences 2012, 109:16004-160011.
13. Roberts A.A., Sharma S.V., Strankman A.W., Duran S.R., Rawat M., Hamilton C.J. Mechanistic studies of FosB: a divalent metal-dependent bacillithiol-S-transferase that mediates fosfomycin resistance in Staphylococcus aureus. Biochemical Journal 2013, 451:69-79.
14. Sharma S.V., Jothivasan V.K., Newton G.L., Upton H., Wakabayashi J.I., Kane M.G., Roberts A.A., Rawat M., La Clair J.J., Hamilton C.J. Chemical and chemoenzymatic syntheses of bacillithiol: a unique low-molecular-weight thiol amongst low G + C Gram-positive bacteria. Angewandte Chemie 2011, 123:7239-7242.
15. Rawat M., Johnson C., Cadiz V., Av-Gay Y. Comparative analysis of mutants in the mycothiol biosynthesis pathway in Mycobacterium smegmatis. Biochemical and Biophysical Research Communications 2007, 363:71-76.
16. Newton G.L., Leung S.S., Wakabayashi J.I., Rawat M., Fahey R.C. The DinB superfamily includes novel mycothiol, bacillithiol, and glutathione S-transferases. Biochemistry 2011, 50:10751-10760.
17. Steffek M., Newton G.L., Av-Gay Y., Fahey R.C. Characterization of Mycobacterium tuberculosis mycothiol-S-conjugate amidase. Biochemistry 2003, 42:12067-12076.
18. Habig W.H., Pabst M.J., Jakoby W.B. Glutathione S-transferases. Journal of Biological Chemistry 1974, 249:7130-7139.
19. Willetts A.J., Turner J.M. Threonine metabolism in a strain of Bacillus subtilis: enzymes acting on methylglyoxal. Biochimica et Biophysica Acta 1970, 222:668-670.
20. Patel M.P., Blanchard J.S. Mycobacterium tuberculosis mycothione reductase: pH dependence of the kinetic parameters and kinetic isotope effects. Biochemistry 2001, 40:5119-5126.
21. Nicholas G.M., Eckman L.L., Newton G.L., Fahey R.C., Ray S., Bewley C.A. Inhibition and kinetics of Mycobacterium tuberculosis and Mycobacterium smegmatis mycothiol-S-conjugate amidase by natural product inhibitors. Bioorganic & Medicinal Chemistry 2003, 11:601-608.
22. Pick N., Rawat M., Arad D., Lan J., Fan J., Kende A.S., Av-Gay Y. In vitro properties of antimicrobial bromotyrosine alkaloids. Journal of Medical Microbiology 2006, 55:407-415.
23. Tan S.-X., Greetham D., Raeth S., Grant C.M., Dawes I.W., Perrone G.G. The thioredoxin-thioredoxin reductase system can function in vivo as an alternative system to reduce oxidized glutathione in Saccharomyces cerevisiae. Journal of Biological Chemistry 2010, 285:6118-6126.
24. Pohl S., Tu W.Y., Aldridge P.D., Gillespie C., Hahne H., Mäder U., Read T.D., Harwood C.R. Combined proteomic and transcriptomic analysis of the response of Bacillus anthracis to oxidative stress. Proteomics 2011, 11:3036-3055.
25. Lee J.-W., Soonsanga S., Helmann J.D. A complex thiolate switch regulates the Bacillus subtilis organic peroxide sensor OhrR. Proceedings of the National Academy of Sciences 2007, 104:8743-8748.