A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa

PLoS ONE

Volumn 8, Issue 6, 2013, Pages

  Kim, Seong Bin ^a   Timmusk, Salme ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; BETA AMYLASE; CHLORAMPHENICOL; ERYTHROMYCIN; PLASMID DNA; BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIUM MUTANT; DNA DENATURATION; GENE DELETION; GENE DISRUPTION; GENE INACTIVATION; GENE INSERTION; GENE TARGETING; HOMOLOGOUS RECOMBINATION; MARKER EXCHANGE MUTAGENESIS; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS POLYMYXA; SCREENING; SUICIDE GENE; BACILLUS SUBTILIS; BIOSYNTHESIS; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; METABOLISM; MUTATION; PLASMID; PROCEDURES;

BACTERIA (MICROORGANISMS); PAENIBACILLUS POLYMYXA;

ALPHA-AMYLASES; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BETA-AMYLASE; CLONING, MOLECULAR; GENE KNOCKOUT TECHNIQUES; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; MUTATION; PAENIBACILLUS POLYMYXA; PLASMIDS; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC;

EID: 84879545611     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0068092     Document Type: Article

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