Efficient production of polymyxin in the surrogate host Bacillus subtilis by introducing a foreign ectB gene and disrupting the abrB gene

Applied and Environmental Microbiology

Volumn 78, Issue 12, 2012, Pages 4194-4199

  Park, Soo Young ^a   Choi, Soo Keun ^a,b   Kim, Jihoon ^a,b   Oh, Tae Kwang ^b,c   Park, Seung Hwan ^a,b  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b University of Science and Technology (UST)   (South Korea)

^c 21C Frontier Microbial Genomics and Applications Center ^*  (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS SUBTILIS; BACILLUS SUBTILIS STRAINS; BIOSYNTHETIC GENE; BIOSYNTHETIC GENE CLUSTER; EFFICIENT PRODUCTION; PAENIBACILLUS POLYMYXA; Q-RT-PCR; QUANTITATIVE REAL TIME PCR; SURROGATE HOST; SYNTHASES; TRANSCRIPTION LEVEL; UPSTREAM REGION;

GENE ENCODING; POLYMERASE CHAIN REACTION;

TRANSCRIPTION;

ABRB PROTEIN, BACILLUS SUBTILIS; BACTERIAL PROTEIN; DNA BINDING PROTEIN; POLYMYXIN; RECOMBINANT PROTEIN; SPO0A PROTEIN, BACILLUS SUBTILIS; TRANSCRIPTION FACTOR;

BACTERIUM; ENZYME ACTIVITY; GENE; HOST; INHIBITION; POLYMERASE CHAIN REACTION; PROTEIN; REAL TIME;

ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE INACTIVATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; PAENIBACILLUS; REAL TIME POLYMERASE CHAIN REACTION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; DNA-BINDING PROTEINS; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE KNOCKOUT TECHNIQUES; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; PAENIBACILLUS; POLYMYXINS; REAL-TIME POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS;

BACILLUS SUBTILIS; PAENIBACILLUS POLYMYXA;

EID: 84864090284     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07912-11     Document Type: Article

References (34)

1. Ainsworth GC, Brown AM, Brownlee G. 1947. Aerosporin, an antibiotic produced by Bacillus aerosporus Greer. Nature 160:263.
2. Choi S-K, et al. 2009. Identification of a polymyxin synthetase gene cluster of Paenibacillus polymyxa and heterologous expression of the gene in Bacillus subtilis. J. Bacteriol. 191:3350-3358.
3. Choi S-K, et al. 2008. Identification and functional analysis of the fusaricidin biosynthetic gene of Paenibacillus polymyxa E681. Biochem. Biophys. Res. Commun. 365:89-95.
4. Cosby WM, Vollenbroich D, Lee OH, Zuber P. 1998. Altered srf expression in Bacillus subtilis resulting from changes in culture pH is dependent on the Spo0K oligopeptide permease and the ComQX system of extracellular control. J. Bacteriol. 180:1438-1445.
5. Greene EA, Spiegelman GB. 1996. The Spo0A protein of Bacillus subtilis inhibits transcription of the abrB gene without preventing binding of the polymerase to the promoter. J. Biol. Chem. 271:11455-11461.
6. Haima P, van Sinderen D, Schotting H, Bron S, Venema G. 1990. Development of a beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis. Gene 86:63-69.
7. Hamon MA, Stanley NR, Britton RA, Grossman AD, Lazazzera BA. 2004. Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis. Mol. Microbiol. 52:847-860.
8. Hanahan D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
9. Holmes KK. 1964. Toxicity of colistin and polymixin B. N. Engl. J. Med. 271:633-634.
10. Jensen K, Ostergaard PR, Wilting R, Lassen SF. 2010. Identification and characterization of a bacterial glutamic peptidase. BMC Biochem. 11:47.
11. Karow ML, Piggot PJ. 1995. Construction of gusA transcriptional fusion vectors for Bacillus subtilis and their utilization for studies of spore formation. Gene 163:69-74.
12. Kaur S, Balakrishnan R, Jayaraman K. 1978. The correlation between antibiotic synthesis, transcription and sporulation in Bacillus polymyxa. Biochem. Biophys. Res. Commun. 81:50-57.
13. Kim JF, et al. 2010. Genome sequence of the polymyxin-producing plantprobiotic rhizobacterium Paenibacillus polymyxa E681. J. Bacteriol. 192: 6103-6104.
14. Kuhlmann AU, Bremer E. 2002. Osmotically regulated synthesis of the compatible solute ectoine in Bacillus pasteurii and related Bacillus spp. Appl. Environ. Microbiol. 68:772-783.
15. Li J, et al. 2006. Colistin: the re-emerging antibiotic for multidrugresistant Gram-negative bacterial infections. Lancet Infect. Dis. 6: 589-601.
16. Lucking G, Dommel MK, Scherer S, Fouet A, Ehling-Schulz M. 2009. Cereulide synthesis in emetic Bacillus cereus is controlled by the transition state regulator AbrB, but not by the virulence regulator PlcR. Microbiology 155:922-931.
17. Molle V, et al. 2003. The Spo0A regulon of Bacillus subtilis. Mol. Microbiol. 50:1683-1701.
18. Nefelova NV, Filippova MS, Egorov NS. 1980. Effect of polymyxins on Bacillus polymyxa sporogenesis. Mikrobiologiia 49:294-297.
19. Palva A, Vigren G, Simonen M, Rintala H, Laamanen P. 1990. Nucleotide sequence of the tetracycline resistance gene of pBC16 from Bacillus cereus. Nucleic Acids Res. 18:1635.
20. Payne DJ, Gwynn MN, Holmes DJ, Pompliano DL. 2007. Drugs for bad bugs: confronting the challenges of antibacterial discovery. Nat. Rev. Drug Discov. 6:29-40.
21. Peterson LR. 2009. Bad bugs, no drugs: no ESCAPE revisited. Clin. Infect. Dis. 49:992-993.
22. Robertson JB, Gocht M, Marahiel MA, Zuber P. 1989. AbrB, a regulator of gene expression in Bacillus, interacts with the transcription initiation regions of a sporulation gene and an antibiotic biosynthesis gene. Proc. Natl. Acad. Sci. U. S. A. 86:8457-8461.
23. Ryu C-M, et al. 2005. Nature of a root-associated Paenibacillus polymyxa from field-grown winter barley in Korea. J. Microbiol. Biotechnol. 15: 984-991.
24. Shin B-S, Choi S-K, Park S-H. 1999. Regulation of the Bacillus subtilis phosphotransacetylase gene. J. Biochem. 126:333-339.
25. Stein T, et al. 2002. Dual control of subtilin biosynthesis and immunity in Bacillus subtilis. Mol. Microbiol. 44:403-416.
26. Strauch M, Webb V, Spiegelman G, Hoch JA. 1990. The Spo0A protein of Bacillus subtilis is a repressor of the abrB gene. Proc. Natl. Acad. Sci. U. S. A. 87:1801-1805.
27. Strauch MA. 1995. In vitro binding affinity of the Bacillus subtilis AbrB protein to six different DNA target regions. J. Bacteriol. 177:4532-4536.
28. Strauch MA, et al. 1989. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J. 8:1615-1621.
29. Studier FW, Moffatt BA. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189: 113-130.
30. Vaara M, Vaara T. 2010. Structure-activity studies on novel polymyxin derivatives that carry only three positive charges. Peptides 31:2318-2321.
31. Vasantha N, Balakrishnan R, Kaur S, Jayaraman K. 1980. Biosynthesis of polymyxin by Bacillus polymyxa. I. The status of the biosynthetic multienzyme complex during active antibiotic synthesis and sporulation. Arch. Biochem. Biophys. 200:40-44.
32. Velkov T, Thompson PE, Nation RL, Li J. 2010. Structure-activity relationships of polymyxin antibiotics. J. Med. Chem. 53:1898-1916.
33. Zavascki AP, Goldani LZ, Li J, Nation RL. 2007. Polymyxin B for the treatment of multidrug-resistant pathogens: a critical review. J. Antimicrob. Chemother. 60:1206-1215.
34. Zhao B, et al. 2006. Cloning and characterization of the genes for biosynthesis of the compatible solute ectoine in the moderately halophilic bacterium Halobacillus dabanensis D-8(T). Curr. Microbiol. 53:183-188.