Evaluation of species-specific PCR, Bruker MS, VITEK MS and the VITEK 2 system for the identification of clinical Enterococcus isolates

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 31, Issue 11, 2012, Pages 3073-3077

  Fang, H ^a   Ohlsson, A K ^a   Ullberg, M ^a   Ozenci V ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; DDL GENE; ENTEROCOCCUS; ENTEROCOCCUS CASSELIFLAVUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS GALLINARUM; GENE SEQUENCE; GROESL GENE; HIGH RESOLUTION MELTING ANALYSIS; INTERMETHOD COMPARISON; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RRNA GENE; SPECIES DIFFERENCE; SPECIES DIFFERENTIATION; VANC1 GENE; VANC2 GENE; VANC3 GENE;

BACTERIAL TYPING TECHNIQUES; BACTERIOLOGICAL TECHNIQUES; COSTS AND COST ANALYSIS; ENTEROCOCCUS; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84867981168     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-012-1667-x     Document Type: Article

References (26)

1. Tan CK, Lai CC, Wang JY, Lin SH, Liao CH, Huang YT, Wang CY, Lin HI, Hsueh PR (2010) Bacteremia caused by non-faecalis and non-faecium Enterococcus species at a Medical center in Taiwan, 2000 to 2008. J Infect 61(1):34-43
2. Na S, Park HJ, Park KH, Cho OH, Chong YP, Kim SH, Lee SO, Sung H, Kim MN, Jeong JY, Kim YS, Woo JH, Choi SH (2012) Enterococcus avium bacteremia: a 12-year clinical experience with 53 patients. Eur J Clin Microbiol Infect Dis 31(3):303-310
3. Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA (2007) Manual of clinical microbiology. American Society for Microbiology Press, Washington, DC, pp 430-442
4. Boyle JF, Soumakis SA, Rendo A, Herrington JA, Gianarkis DG, Thurberg BE, Painter BG (1993) Epidemiologic analysis and genotypic characterization of a nosocomial outbreak of vancomycin-resistant enterococci. J Clin Microbiol 31(5):1280-1285
5. Deshpande LM, Fritsche TR, Moet GJ, Biedenbach DJ, Jones RN (2007) Antimicrobial resistance and molecular epidemiology of vancomycin-resistant enterococci from North America and Europe: a report from the SENTRY antimicrobial surveillance program. Diagn Microbiol Infect Dis 58(2):163-170
6. Low DE, Keller N, Barth A, Jones RN (2001) Clinical prevalence, antimicrobial susceptibility, and geographic resistance patterns of enterococci: results from the SENTRY Antimicrobial Surveillance Program, 1997-1999. Clin Infect Dis 32(Suppl 2):S133-S145
7. Singer DA, Jochimsen EM, Gielerak P, Jarvis WR (1996) Pseudo-outbreak of Enterococcus durans infections and colonization associated with introduction of an automated identification system software update. J Clin Microbiol 34(11):2685-2687
8. Tsakris A, Woodford N, Pournaras S, Kaufmann M, Douboyas J (1998) Apparent increased prevalence of high-level aminoglycoside-resistant Enterococcus durans resulting from false identification by a semiautomated software system. J Clin Microbiol 36(5):1419-1421
9. Angeletti S, Lorino G, Gherardi G, Battistoni F, De Cesaris M, Dicuonzo G (2001) Routine molecular identification of enterococci by gene-specific PCR and 16S ribosomal DNA sequencing. J Clin Microbiol 39(2):794-797
10. Dutka-Malen S, Evers S, Courvalin P (1995) Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 33(1):24-27
11. Tsai JC, Hsueh PR, Lin HM, Chang HJ, Ho SW, Teng LJ (2005) Identification of clinically relevant Enterococcus species by direct sequencing of groES and spacer region. J Clin Microbiol 43(1):235-241
12. Bizzini A, Greub G (2010) Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin Microbiol Infect 16(11):1614-1619
13. Benagli C, Rossi V, Dolina M, Tonolla M, Petrini O (2011) Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. PLoS One 6(1):e16424
14. Jin WY, Jang SJ, Lee MJ, Park G, Kim MJ, Kook JK, Kim DM, Moon DS, Park YJ (2011) Evaluation of VITEK 2, MicroScan, and Phoenix for identification of clinical isolates and reference strains. Diagn Microbiol Infect Dis 70(4):442-447
15. Cha CH, An HK, Kim JU (2010) Detection of vancomycin-resistant enterococci using multiplex real-time PCR assay and melting curve analysis. Korean J Lab Med 30(2):138-146
16. Harmsen D, Rothgänger J, Frosch M, Albert J (2002) RIDOM: Ribosomal Differentiation of Medical Micro-organisms Database. Nucleic Acids Res 30(1):416-417
17. Brosius J, Palmer ML, Kennedy PJ, Noller HF (1978) Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75(10):4801-4805
18. Fang H, Ohlsson AK, Jiang GX, Ullberg M (2012) Screening for vancomycin-resistant enterococci: an efficient and economical laboratory-developed test. Eur J Clin Microbiol Infect Dis 31(3):261-265
19. Fang H, Nord CE, Ullberg M (2010) Screening for vancomycin-resistant enterococci: results of a survey in Stockholm. APMIS 118(5):413-417
20. Hidron AI, Edwards JR, Patel J, Horan TC, Sievert DM, Pollock DA, Fridkin SK; National Healthcare Safety Network Team; Participating National Healthcare Safety Network Facilities (2008) NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006-2007. Infect Control Hosp Epidemiol 29(11):996-1011
21. Zhu X, Zheng B, Wang S, Willems RJ, Xue F, Cao X, Li Y, Bo S, Liu J (2009) Molecular characterisation of outbreak-related strains of vancomycin-resistant Enterococcus faecium from an intensive care unit in Beijing, China. J Hosp Infect 72(2):147-154
22. Willems RJ, Top J, van Santen M, Robinson DA, Coque TM, Baquero F, Grundmann H, Bonten MJ (2005) Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex. Emerg Infect Dis 11(6):821-828
23. Hsieh YC, Lee WS, Ou TY, Hsueh PR (2010) Clonal spread of CC17 vancomycin-resistant Enterococcus faecium with multilocus sequence type 78 (ST78) and a novel ST444 in Taiwan. Eur J Clin Microbiol Infect Dis 29(1):25-30
24. Tyrrell GJ, Turnbull L, Teixeira LM, Lefebvre J, Carvalho Mda G, Facklam RR, Lovgren M (2002) Enterococcus gilvus sp. nov. and Enterococcus pallens sp. nov. isolated from human clinical specimens. J Clin Microbiol 40(4):1140-1145
25. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49(4):543-551
26. Cherkaoui A, Hibbs J, Emonet S, Tangomo M, Girard M, Francois P, Schrenzel J (2010) Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. J Clin Microbiol 48(4):1169-1175