Microbial life associated with low-temperature alteration of ultramafic rocks in the Leka ophiolite complex

Geobiology

Volumn 11, Issue 4, 2013, Pages 318-339

  Daae, F L ^a   Okland I ^a   Dahle, H ^a   Jorgensen S L ^a   Thorseth, I H ^a   Pedersen, R B ^a  

^a UNIVERSITY OF BERGEN   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

MINERAL;

BACTERIUM; CLONE; CONTINENTAL MARGIN; DUNITE; GENE; GEOMICROBIOLOGY; HETEROTROPHY; LOW TEMPERATURE; MICROBIAL ACTIVITY; MICROBIAL COMMUNITY; MICROBIAL MAT; OCEANIC LITHOSPHERE; OPHIOLITE; POLYMERASE CHAIN REACTION; SERPENTINIZATION; ULTRAMAFIC ROCK; WATER-ROCK INTERACTION;

ARTICLE; BIOTA; CHEMISTRY; CLUSTER ANALYSIS; DNA SEQUENCE; METAGENOMICS; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MICROSCOPY; MOLECULAR GENETICS; NORWAY; NUCLEOTIDE SEQUENCE; PHYLOGENY; REAL TIME POLYMERASE CHAIN REACTION; SEDIMENT;

BIOTA; CLUSTER ANALYSIS; GEOLOGIC SEDIMENTS; METAGENOMICS; MICROBIOLOGICAL TECHNIQUES; MICROSCOPY; MINERALS; MOLECULAR SEQUENCE DATA; NORWAY; PHYLOGENY; REAL-TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

NORWAY;

BACTERIA (MICROORGANISMS); HYDROGENOPHAGA;

EID: 84879415759     PISSN: 14724677     EISSN: 14724669     Source Type: Journal    
DOI: 10.1111/gbi.12035     Document Type: Article

References (103)

1. Abrajano TA, Sturchio NC, Kennedy BM, Lyon GL, Muehlenbachs K, Bohlke JK (1990) Geochemistry of reduced gas related to serpentinization of the Zambales ophiolite, Philippines. Applied Geochemistry 5, 625-630.
2. Ahmadjian V (1988) The lichen alga Trebouxia: does it occur free-living? Plant Systematics and Evolution 158, 243-247.
3. Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W, Lipman D (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389-3402.
4. Amann RI, Ludwig W, Schleifer KH (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Reviews 59, 143-169.
5. oN (ODP Leg 209, Site 1274). Geophysical Research Letters 33, L13306.
6. Barnes I, La Marché VR Jr, Himmelberg G (1967) Geochemical evidence of present-day serpentinization. Science 156, 830-832.
7. Blank JG, Green SJ, Blake D, Valley JW, Kita NT, Treiman A, Dobson PF (2009) An alkaline spring system within the Del Puerto Ophiolite (California, USA): a Mars analog site. Planetary and Space Science 57, 533-540.
8. Brazelton WJ, Schrenk MO, Kelley DS, Baross JA (2006) Methane- and sulfur-metabolizing microbial communities dominate the lost city hydrothermal field ecosystem. Applied and Environment Microbiology 72, 6257-6270.
9. 2 production by serpentinite-hosted subsurface microbial communities. Frontiers in Microbiology 2, 268.
10. 2+-oxidizing bacteria and fungi isolated from Mn nodules in rice field subsoils. Biology and Fertility of Soils 45, 337-346.
11. Cannat M, Mevel C, Maia M, Deplus C, Durand C, Gente P, Agrinier P, Belarouchi A, Dubuisson G, Humler E, Reynolds J (1995) Thin crust, ultramafic exposures, and rugged faulting patterns at Mid-Atlantic Ridge (22°-24°N). Geology 23, 49-52.
12. o 20' N fracture zone and the Mid-Atlantic Ridge. Geochimica et Cosmochimica Acta 62, 2323-2333.
13. 4 vent fluids issuing from ultramafic rocks at the Rainbow hydrothermal field (36° 14′N, MAR). Chemical Geology 191, 345-359.
14. Connon SA, Tovanabootr A, Dolan M, Vergin K, Giovannoni SJ, Semprini L (2005) Bacterial community composition determined by culture-independent and -dependent methods during propane-stimulated bioremediation in trichloroethene-contaminated groundwater. Environmental Microbiology 7, 165-178.
15. Daae FL (2006) Mikrobielt Liv Assosiert Med Forvitring av Ultramafiske Bergarter. Master thesis, University of Bergen, Norway.
16. Daims HBA, Amann R, Schleifer Kh, Wagner M (1999) The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. Systematic and Applied Microbiology 22, 434-444.
17. Davis JB, Chase HH, Raymond RL (1956) Mycobacterium paraffinicum n. sp., a bacterium isolated from soil. Applied Microbiology 4, 310-315.
18. Dawson SC, Pace NR (2002) Novel kingdom-level eukaryotic diversity in anoxic environments. Proceedings of the National Academy of Sciences of the USA 99, 8324-8329.
19. DeGrood SH, Claassen VP, Scow KM (2005) Microbial community composition on native and drastically disturbed serpentine soils. Soil Biology and Biochemistry 37, 1427-1435.
20. DeLong E (1992) Archaea in coastal marine environments. PNAS 89, 5685-5689.
21. Dunfield PF, Yuryev A, Senin P, Smirnova AV, Stott MB, Hou S, Ly B, Saw JH, Zhou Z, Ren Y, Wang J, Mountain BW, Crowe MA, Weatherby TM, Bodelier PLE, Liesack W, Feng L, Wang L, Alam M (2007) Methane oxidation by an extremely acidophilic bacterium of the phylum Verrucomicrobia. Nature 450, 879-882.
22. Edwards U, Rogall T, Blocker H, Emde M, Bottger EC (1989) Isolation and direct complete nucleotide determination of entire genes - characterization of a gene coding for 16S-ribosomal RNA. Nucleic Acids Research 17, 7843-7853.
23. Ehrich S, Behrens D, Lebedeva E, Ludwig W, Bock E (1995) A new obligately chemolithoautotrophic, nitrite-oxidizing bacterium, Nitrospira moscoviensis sp. nov. and its phylogenetic relationship. Archives of Microbiology 164, 16-23.
24. Einen J, Thorseth IH, Øvreås L (2008) Enumeration of Archaea and Bacteria in seafloor basalt using real-time quantitative PCR and fluorescence microscopy. FEMS Microbiology Letters 282, 182-187.
25. Foustoukos DI, Seyfried WE (2004) Hydrocarbons in hydrothermal vent fluids: the role of chromium-bearing catalysts. Science 304, 1002-1005.
26. Furnes H, Pedersen RB, Stillman CJ (1988) The Leka Opholite Complex, central Norwegian Caledonides: field characteristics and geotectonic significance. Journal of the Geological Society 145, 401-412.
27. Glockner FO, Amann R, Alfreider A, Pernthaler J, Psenner R, Trebesius K, Schleifer KH (1996) An in situ hybridization protocol for detection and identification of planktonic bacteria. Systematic and Applied Microbiology 19, 403-406.
28. Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52, 696-704.
29. Haggerty JA (1991) Evidence from fluid seeps atop serpentine seamounts in the Mariana Forearc: clues for emplacement of the seamounts and their relationships to forearcs tectonics. Marine Geology 102, 293-309.
30. Heberling C, Lowell RP, Liu L, Fisk MR (2010) Extent of the microbial biosphere in the oceanic crust. Geochemistry, Geophysics, Geosystems 11, Q08003.
31. Hedrich S, Schlömann M, Johnson DB (2011) The iron-oxidizing proteobacteria. Microbiology 157, 1551-1564.
32. Heinzel E, Janneck E, Glombitza F, Schlömann M, Seifert J (2009) Population dynamics of iron-oxidizing communities in pilot plants for the treatment of acid mine waters. Environmental Science and Technology 43, 6138-6144.
33. Herrmann M, Saunders AM, Schramm A (2008) Archaea dominate the ammonia-oxidizing community in the rhizosphere of the freshwater macrophyte Littorella uniflora. Applied and Environment Microbiology 74, 3279-3283.
34. Holm NG, Charlou JL (2001) Initial indications of abiotic formation of hydrocarbons in the Rainbow ultramafic hydrothermal system, Mid-Atlantic Ridge. Earth and Planetary Science Letters 191, 1-8.
35. Holm N, Neubeck A (2009) Reduction of nitrogen compounds in oceanic basement and its implications for HCN formation and abiotic organic synthesis. Geochemical Transactions 10, 9.
36. Horita J, Berndt ME (1999) Abiogenic methane formation and isotopic fractionation under hydrothermal conditions. Science 285, 1055-1057.
37. Huber TFG, Hugenholtz P (2004) Bellerophon: a program to detect chimeric sequences in multiple sequence alignments. Bioinformatics 20, 2317-2319.
38. Islam T, Jensen S, Reigstad LJ, Larsen Ø, Birkeland N-K (2008) Methane oxidation at 55°C and pH 2 by a thermoacidophilic bacterium belonging to the Verrucomicrobia phylum. Proceedings of the National Academy of Sciences of the USA 105, 300-304.
39. Janecky DR, Seyfried WE (1986) Hydrothermal serpentinization of peridotite within the oceanic crust: experimental investigations of mineralogy and major element chemistry. Geochimica et Cosmochimica Acta 50, 1357-1378.
40. Jorgensen SL, Hannisdal B, Lanzen A, Baumberger T, Flesland K, Fonseca R, Ovreas L, Steen IH, Thorseth IH, Pedersen RB, Schleper C (2012) Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge. Proceedings of the National Academy of Sciences of the USA 109, E2846-E2855.
41. Jurgens G, Lindstrom K, Saano A (1997) Novel group within the kingdom Crenarchaeota from boreal forest soil. Applied and Environment Microbiology 63, 803-805.
42. Kalyuzhnaya MG, De Marco P, Bowerman S, Pacheco CC, Lara JC, Lidstrom ME, Chistoserdova L (2006) Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates. International Journal of Systematic and Evolutionary Microbiology 56, 2517-2522.
43. Karavaiko G, Dubinina G, Kondrat'eva T (2006) Lithotrophic microorganisms of the oxidative cycles of sulfur and iron. Microbiology 75, 512-545.
44. Kasai Y, Takahata Y, Hoaki T, Watanabe K (2005) Physiological and molecular characterization of a microbial community established in unsaturated, petroleum contaminated soil. Environmental Microbiology 7, 806-818.
45. Kelley DS, Karson JA, Blackman DK, Fruh-Green GL, Butterfield DA, Lilley MD, Olson EJ, Schrenk MO, Roe KK, Lebon GT, Rivizzigno P (2001) An off-axis hydrothermal vent field near the Mid-Atlantic Ridge at 30 degrees N. Nature 412, 145-149.
46. Kelley DS, Karson JA, Fruh-Green GL, Yoerger DR, Shank TM, Butterfield DA, Hayes JM, Schrenk MO, Olson EJ, Proskurowski G, Jakuba M, Bradley A, Larson B, Ludwig K, Glickson D, Buckman K, Bradley AS, Brazelton WJ, Roe K, Elend MJ, Delacour A, Bernasconi SM, Lilley MD, Baross JA, Summons RT, Sylva SP (2005) A serpentinite-hosted ecosystem: the lost city hydrothermal field. Science 307, 1428-1434.
47. Kim J, Crowley DE (2007) Microbial diversity in natural asphallts of the Rancho La Brea Tar Pits. Applied and Environment Microbiology 78, 4579-4591.
48. Kirchman DL (2002) The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiology Ecology 39, 91-100.
49. Klappenbach JA, Saxman PR, Cole JR, Schmidt TM (2001) rrndb: the ribosomal RNA operon copy number database. Nucleic Acids Research 29, 181-184.
50. Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR (1985) Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. PNAS 82, 6955-6959.
51. Larsen EI, Sly LI, Mcewan AG (1999) Manganese(II) adsorption and oxidation by whole cells and a membrane fraction of Pedomicrobium sp. ACM 3067. Archives of Microbiology 171, 257-264.
52. Lee ZM-P, Bussema C, Schmidt TM (2009) rrnDB: documenting the number of rRNA and tRNA genes in bacteria and archaea. Nucleic Acids Research 37, D489-D493.
53. Lenczewski M, Rigg L, Enright N, Jaffre T, Kelly H (2009) Microbial communities of ultramafic soils in maquis and rainforest at Mont Do, New Caledonia. Austral Ecology 34, 567-576.
54. Li Y, Montgomery-Brown J, Reinhard M (2010) Biotransformation of halogenated nonylphenols with Sphingobium xenophagum bayram and a nonylphenol-degrading soil-enrichment culture. Archives of Environmental Contamination and Toxicology 60, 1-8.
55. Loy A, Lehner A, Lee N, Adamczyk J, Meier H, Ernst J, Schleifer K-H, Wagner M (2002) Oligonucleotide microarray for 16S rRNA Gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment. Applied and Environment Microbiology 68, 5064-5081.
56. Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhu K, Buchner A, Lai T, Steppi S, Jobb G, Forster W, Brettske I, Gerber S, Ginhart AW, Gross O, Grumann S, Hermann S, Jost R, Konig A, Liss T, Lussmann R, May M, Nonhoff B, Reichel B, Strehlow R, Stamatakis A, Stuckmann N, Vilbig A, Lenke M, Ludwig T, Bode A, Schleifer K-H (2004) ARB: a software environment for sequence data. Nucleic Acids Research 32, 1363-1371.
57. Lysnes K, Thorseth IH, Steinsbu BO, Øvreås L, Torsvik T, Pedersen RB (2004) Microbial community diversity in seafloor basalt from the Arctic spreading ridges. FEMS Microbiology Ecology 50, 213-230.
58. Mason OU, Nakagawa T, Rosner M, Van Nostrand JD, Zhou J, Maruyama A, Fisk MR, Giovannoni SJ (2010) First investigation of the microbiology of the deepest layer of ocean crust. PLoS ONE 5, e15399.
59. Massana R, Murray AE, Preston CM, Delong EF (1997) Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. Applied and Environmental Microbiology 63, 50-56.
60. McCollom TM, Bach W (2009) Thermodynamic constraints on hydrogen generation during serpentinization of ultramafic rocks. Geochimica et Cosmochimica Acta 73, 856-875.
61. 2 to hydrocarbons during serpentinization of olivine. Geochimica et Cosmochimica Acta 65, 3769-3778.
62. Menez B, Pasini V, Brunelli D (2012) Life in the hydrated suboceanic mantle. Nature Geoscience 5, 133-137.
63. Militon C, Boucher D, Vachelard C, Perchet G, Barra V, Troquet J, Peyretaillade E, Peyret P (2010) Bacterial community changes during bioremediation of aliphatic hydrocarbon contaminated soil. FEMS Microbiology Ecology 74, 669-681.
64. Moody JB (1976) An experimental study of the serpentinization of iron-bearing olivines. Canadian Mineralogist 14, 462-478.
65. Morikawa K, Yanagida M (1981) Visualization of individual DNA molecules in solution by light microscopy: DAPI staining method. Journal of Biochemistry 89, 693-696.
66. Muyzer G, Dewaal EC, Uitterlinden AG (1993) Profiling of complex microbial-populations by denaturing gradient gel-electrophoresis analysis of polymerase chain reaction-amplified genes-coding for 16S ribosomal-RNA. Applied and Environmental Microbiology 59, 695-700.
67. Neal C, Stanger G (1983) Hydrogen generation from mantle source rocks in Oman. Earth and Planetary Science Letters 66, 315-320.
68. Nealson KH, Inagaki F, Takai K (2005) Hydrogen-driven subsurface lithoautotrophic microbial ecosystems (SLiMEs): do they exist and why should we care? Trends in Microbiology 13, 405-410.
69. Neubeck A, Duc N, Bastviken D, Crill P, Holm N (2011) Formation of H2 and CH4 by weathering of olivine at temperatures between 30 and 70 degrees C. Geochemical Transactions 12, 6.
70. Nielsen PH, Kragelund C, Seviour RJ, Nielsen JL (2009) Identity and ecophysiology of filamentous bacteria in activated sludge. FEMS Microbiology Reviews 33, 969-998.
71. Okland I, Huang S, Dahle H, Thorseth IH, Pedersen RB (2012) Low temperature alteration of serpentinized ultramafic rock and implications for microbial life. Chemical Geology 318, 75-87.
72. Oksanen J, Blanchet FG, Kindt R, Legendre P, O'Hara RB, Simpson GL, Solymos P, Stevens MHH, Wagner H (2011). Vegan: Community Ecology Package. R package version 1.17-6. http://vegan.r-forge.r-project.org/
73. Oline DK (2006) Phylogenetic comparisons of bacterial communities from serpentine and nonserpentine soils. Applied and Environment Microbiology 72, 6965-6971.
74. Orcutt BN, Joye SB, Kleindienst S, Knittel K, Ramette A, Reitz A, Samarkin V, Treude T, Boetius A (2010) Impact of natural oil and higher hydrocarbons on microbial diversity, distribution and activity in Gulf of Mexico cold-seep sediments. Deep Sea Research Part II: Topical Study in Oceanograpy 57, 2008-2021.
75. Osaka T, Ebie Y, Tsuneda S, Inamori Y (2008) Identification of the bacterial community involved in methane-dependent denitrification in activated sludge using DNA stable-isotope probing. FEMS Microbiology Ecology 64, 494-506.
76. Palandri JL, Reed MH (2004) Geochemical models of metasomatism in ultramafic systems: serpentinization, rodingitization, and sea floor carbonate chimney precipitation. Geochimica Et Cosmochimica Acta 68, 1115-1133.
77. Philippot P, Busigny V, Scambelluri M, Cartigny P (2007) Oxygen and nitrogen isotopes as tracers of fluid activities in serpentinites and metasediments during subduction. Mineralogy and Petrology 91, 11-24.
78. Proskurowski G, Lilley MD, Kelley DS, Olson EJ (2006) Low temperature volatile production at the Lost City Hydrothermal Field, evidence from a hydrogen stable isotope geothermometer. Chemical Geology 229, 331-343.
79. Proskurowski G, Lilley MD, Seewald JS, Fruh-Green GL, Olson EJ, Lupton JE, Sylva SP, Kelley DS (2008) Abiogenic hydrocarbon production at Lost City hydrothermal field. Science 319, 604-607.
80. Prosser JI, Nicol GW (2008) Relative contributions of archaea and bacteria to aerobic ammonia oxidation in the environment. Environmental Microbiology 10, 2931-2941.
81. Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J, Glöckner FO (2007) SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Research 35, 7188-7196.
82. Reese Næsborg R, Ekman S, Tibell L (2007) Molecular phylogeny of the genus Lecania (Ramalinaceae, lichenized Ascomycota). Mycological Research 111, 581-591.
83. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environment Microbiology 75, 7537-7541.
84. Schulte MBD, Hoehler T, McCollom T (2006) Serpentinization and its implications for life on the early Earth and Mars. Astrobiology 6, 364-376.
85. 2-Metabolizing Prokaryotes. The Prokaryotes, Springer, New York, NY, pp. 496-563.
86. 2-rich fluids from serpentinization: geochemical and biotic implications. Proceedings of the National Academy of Sciences of the USA 101, 12818-12823.
87. Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiology Today 33, 152-155.
88. Stackebrandt E, Schumann P, Dworkin M, Falkow S, Rosenberg E, Schleifer K-H (2006) Introduction to the Taxonomy of Actinobacteria the Prokaryotes, Springer, New York, NY, pp. 297-321.
89. Stein LY, Jones G, Alexander B, Elmund K, Wright-Jones C, Nealson KH (2002) Intriguing microbial diversity associated with metal-rich particles from a freshwater reservoir. FEMS Microbiology Ecology 42, 431-440.
90. Stevens TO, Mckinley JP (1995) Lithoautotrophic microbial ecosystems in deep basalt aquifers. Science 270, 450-455.
91. Stoecker K, Dorninger C, Daims H, Wagner M (2010) Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) improves signal intensity and increases rRNA accessibility. Applied and Environment Microbiology 76, 922-926.
92. Szponar N, Brazelton WJ, Schrenk MO, Bower DM, Steele A, Morrill PL (2012). Geochemistry of a continental site of serpentinization, the Tablelands Ophiolite, Gros Morne National Park: a Mars analogue. Icarus, in press. http://dx.doi.org/10.1016/j.icarus.2012.07.004.
93. Tanaka Y, Yoshida T, Watanabe K, Izumi Y, Mitsunaga T (1997) Cloning and analysis of methanol oxidation genes in the methylotroph Hyphomicrobium methylovorum GM2. FEMS Microbiology Letters 154, 397-401.
94. Thomsen TR, Nielsen JL, Ramsing NB, Nielsen PH (2004) Micromanipulation and further identification of FISH-labelled microcolonies of a dominant denitrifying bacterium in activated sludge. Environmental Microbiology 6, 470-479.
95. Tiago I, Chung AP, Verissimo A (2004) Bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. Applied and Environment Microbiology 70, 7378-7387.
96. Treusch AH, Leininger S, Kletzin A, Schuster SC, Klenk HP, Schleper C (2005) Novel genes for nitrite reductase and Amo-related proteins indicate a role of uncultivated mesophilic crenarchaeota in nitrogen cycling. Environmental Microbiology 7, 1985-1995.
97. Van Veen W (1973) Biological oxidation of manganese in soils. Antonie van Leeuwenhoek 39, 657-662.
98. Wallner G, Amann R, Beisker W (1993) Optimizing fluorescent in situ-hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14, 136-143.
99. Willems A, Busse J, Goor M, Pot B, Falsen E, Jantzen E, Hoste B, Gillis M, Kersters K, Auling G, De Ley J (1989) Hydrogenophaga, a new genus of hydrogen-oxidizing bacteria that includes Hydrogenophaga flava comb. nov. (Formerly Pseudomonas flava), Hydrogenophaga palleronii (Formerly Pseudomonas palleronii), Hydrogenophaga pseudoflava (Formerly Pseudomonas pseudoflava and "Pseudomonas carboxydoflava"), and Hydrogenophaga taeniospiralis (Formerly Pseudomonas taeniospiralis). International Journal of Systematic Bacteriology 39, 319-333.
100. Yagi JM, Neuhauser EF, Ripp JA, Mauro DM, Madsen EL (2009) Subsurface ecosystem 11 resilience: long-term attenuation of subsurface contaminants supports a dynamic 12 microbial community. ISME Journal 4, 131-143.
101. Yamamoto N, Asano R, Yoshii H, Kenichi O, Nakai Y (2011) Archaeal community dynamics and detection of ammonia-oxidizing archaea during composting of cattle manure using culture-independent DNA analysis. Applied Microbiology and Biotechnology 90, 1501-1510.
102. Ying JY, Zhang LM, He JZ (2010) Putative ammonia-oxidizing bacteria and archaea in an acidic red soil with different land utilization patterns. Environmental Microbiology Reports 2, 304-312.
103. Zehr JP, Jenkins BD, Short SM, Steward GF (2003) Nitrogenase gene diversity and microbial community structure: a cross-system comparison. Environmental Microbiology 5, 539-554.