Adenosine receptor expression and gene reference evaluation in human leukocytes

Clinical Laboratory

Volumn 59, Issue 5-6, 2013, Pages 571-577

  Sabatino, Laura ^a   Cabiati, Manuela ^a   Caselli, Chiara ^a   Del Ry, Silvia ^a  

^a INSTITUTE OF CLINICAL PHYSIOLOGY   (Italy)

Author keywords

Adenosine receptor; Leukocytes; mRNA expression; Real time PCR; Reference gene

Indexed keywords

ADENOSINE A1 RECEPTOR; ADENOSINE A2 RECEPTOR; ADENOSINE A2A RECEPTOR; ADENOSINE A2B RECEPTOR; ADENOSINE A3 RECEPTOR; ADENOSINE RECEPTOR; EEF1A PROTEIN; MESSENGER RNA; RIBONUCLEASE; RIBOSOME RNA; RNA 18S; RNA 28S; RPL13A PROTEIN; TPT1 PROTEIN; UNCLASSIFIED DRUG;

AGAR GEL ELECTROPHORESIS; ARTICLE; BLOOD; BLOOD SAMPLING; CONTROLLED STUDY; GENE EXPRESSION; GENE SYNTHESIS; GENETIC SELECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; LEUKOCYTE; MOUSE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; REFERENCE DATABASE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION;

ADULT; ANALYSIS OF VARIANCE; ANIMALS; ELECTROPHORESIS, AGAR GEL; GENETIC MARKERS; HUMANS; LEUKOCYTES; MICE; REAL-TIME POLYMERASE CHAIN REACTION; RECEPTORS, PURINERGIC P1; REFERENCE VALUES; RNA, MESSENGER;

EID: 84879407215     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: 10.7754/Clin.Lab.2012.120219     Document Type: Article

References (24)

1. Fredholm BB, Ijzerman AP, Jacobson KA, Klotz KN, Linden J. International Union of Pharmacology XXV Nomenclature and classification of adenosine receptors. Pharmacol Rev 2001;53:527-52. (Pubitemid 33136237)
2. Van Belle H, Goossens F, Wynants J. Formation and release of purine catabolites during hypoperfusion, anoxia and ischemia. Am J Physiol 1987;252:H886-93.
3. Cronstein BN, Levin RI, Belanoff J, Weissmann G, Hirschhorn R. Adenosine: an endogenous inhibitor of neutrophil-mediated injury to endothelial cells. J Clin Invest 1986;78:760-70. (Pubitemid 16024813)
4. Montesinos MC, Cronstein BN. Role of P1 receptor in inflammation. In: Williams MPAM (ed). Handbook of experimental pharmacology, Berlin: Springer; 2001 p. 303-15.
5. Sullivan GW, Linden J. Role of A2A adenosine receptors in inflammation. Drug Dev Res 1998;45:103-12. (Pubitemid 29119592)
6. Sitkovsky MV. Use of the A(2A) adenosine receptor as a physiological immuno suppressor and to engineer inflammation in vivo. Bioch Pharmacol 2003;65:493-501. (Pubitemid 36144234)
7. Firestein GS. Anti-inflammatory effects of adenosine kinase inibithors in acute and chronic inflammation. Drug Dev Res 1996;39:371-6. (Pubitemid 27199347)
8. 2a receptors inhibits IL-12 production by human monocytes. J Immunol 2000;164:436-42.
9. Meldrum DR, Dinarello CA, Shames BD, et al. Adenosine decreases post-ischemic TNF-α production: anti-inflammatory implications for preconditioning and transplantation. Immunology 1997;92:472-7. (Pubitemid 27525218)
10. Del Ry S, Cabiati M, Martino A, Simioniuc A, Morales MA, Picano E. Adenosine receptor mRNA expression in normal and failing minipig hearts. J Cardiovasc Pharmacol 2011;58:149-56.
11. Kitakaze M, Minamino T, Node K, et al. Elevation of plasma adenosine levels may attenuate the severity of chronic heart failure. Cardiovasc Drugs Ther 1998;12:307-9.
12. Picano E. Dipyridamole in myocardial ischemia: Good Samaritan or Terminator? International J Cardiology 2002;83:215-6. (Pubitemid 34564503)
13. Brodde OE, Beckering JJ, Michel MC: Human heart β-adrenoceptors: a fair comparison with lymphocyte β-adrenoceptors? Trend Pharmacol Sci 1987;8:403-7.
14. 2 adenosine receptors in chronic heart failure and cardiac transplantation. FASEB J 2003;17:280-2.
15. Capecchi PL, Camurri A, Pompella G, et al. Upregulation of A2A adenosine receptor expression by TNF-α in PBMC of patients with CHF: a regulatory mechanism of inflammation. J Card Fail 2005;11:67-73. (Pubitemid 40215317)
16. Fortin A, Harbour D, Fernandes M, Borgeat P, Bourgin S. Differential expression of adenosine receptors in human neutrophils: up-regulation by specific Th1 cytokines and lipopolysaccharide. J leukoc Biol 2006;79:574-5.
17. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocr 2000;25:169-93.
18. Hendriks-Balk MC, Michel MC, Alewijnse AE. Pitfalls in the normalization of real-time polymerase chain reaction data. Basic Res Cardiology 2007;102:195-7. (Pubitemid 46564068)
19. Huggett J, Dheda K, Bustin S, Zumla A. Real-time RT-PCR normalization; strategies and considerations. Genes and Immunity 2005;6:279-84. (Pubitemid 40903399)
20. Brattelid T, Tveit K, Birkeland JA, et al. Expression of mRNA encoding G protein-coupled receptors involved in congestive heart failure - a quantitative RT-PCR study and the question of normalization. Basic Res Cardiology 2007;102:198-08.
21. de Jonge HJ, Fehrmann RS, de Bont ES, et al. Evidence based selection of housekeeping genes. PLoS ONE 2007;2:e898.
22. Schmittgen TD, Zakrajsek BA. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J Biochem Biophys Met 2000;46:69-81.
23. Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genom Biol 2002; 3:RESEARCH0034.
24. Martino A, Cabiati M, Campan M, et al. Selection of reference genes for normalization of Real-Time PCR data in minipig Heart Failure model and evaluation of TNF-α mRNA expression. J Biotechnol 2011;153:92-9.