Gene replacement and elimination using λred- and FLP-based tool to re-direct carbon flux in acetogen biocatalyst during continuous CO 2/H2 blend fermentation

Journal of Industrial Microbiology and Biotechnology

Volumn 40, Issue 7, 2013, Pages 749-758

  Tyurin, Michael ^a  

^a Syngas Biofuels Energy Inc   (United States)

Author keywords

Acetogens; Continuous fermentation; Formate; Gene elimination; Gene replacement; Mevalonate

Indexed keywords

ACETALDEHYDE; ACETIC ACID; ACETOGENIN; ALCOHOL; ALCOHOL DEHYDROGENASE; CARBON DIOXIDE; FORMIC ACID; HYDROGEN; MEVALONIC ACID;

ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BIOCATALYST; CELL ENERGY; CLOSTRIDIUM; FERMENTATION; GENE CLUSTER; GENE EXPRESSION; GENE REPLACEMENT THERAPY; GENOME SIZE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPO0A GENE;

ACETALDEHYDE; ACETIC ACID; ALCOHOL DEHYDROGENASE; BIOCATALYSIS; BIOREACTORS; CARBON CYCLE; CARBON DIOXIDE; CELL PROLIFERATION; CLOSTRIDIUM; DNA NUCLEOTIDYLTRANSFERASES; ETHANOL; FERMENTATION; FORMATE DEHYDROGENASES; FORMATES; GENE DELETION; GENES, BACTERIAL; GENOME, BACTERIAL; HYDROGEN; HYDROGENASE; MEVALONIC ACID; MULTIENZYME COMPLEXES; MUTAGENESIS, INSERTIONAL; RECOMBINASES;

CLOSTRIDIUM SP.;

EID: 84879229209     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-013-1279-1     Document Type: Article

References (32)

1. Abubackar HN, Veiga MC, Kennes C (2012) Biological conversion of carbon monoxide to ethanol: effect of pH, gas pressure, reducing agent and yeast extract. Bioresource Technol 114:518-522
2. Berzin V, Tyurin M (2012) Acetogen biocatalyst Clostridium sp. MTEtOH871 engineered with our proprietary electrotransformation technology and equipment: continuous synthesis gas fermentation for selective ethanol production. J Biotech Res 4:54-64
3. Berzin V, Kiriukhin M, Tyurin M (2012) Elimination of acetate production to improve ethanol yield during continuous synthesis gas fermentation by engineered biocatalyst Clostridium sp. MTEtOH550. Appl Biochem Biotechnol 167(2):338-347. doi: 10.1007/s12010-012-9697-5
4. Berzin V, Kiriukhin M, Tyurin M (2012) Cre-lox66/lox71-based elimination of phosphotransacetylase or acetaldehyde dehydrogenase shifted carbon flux in acetogen rendering selective overproduction of ethanol or acetate. Appl Biochem Biotechnol 195(3):181-188. doi: 10.1007/s12010-012-9864-8
5. Berzin V, Kiriukhin M, Tyurin M (2012) Selective production of acetone during continuous synthesis gas fermentation by engineered biocatalyst Clostridium sp. MAceT113. Lett Appl Microbiol 55(2):149-54. doi: 10.1111/j.1472-765X.2012.03272.x
6. Berzin V, Kiriukhin M, Tyurin M (2013) "Curing" of plasmid DNA in acetogen using microwave or applying an electric pulse improves cell growth and metabolite production as compared to the plasmid-harboring strain. Arch Microbiol 195(3):181-188. doi: 10.1007/s00203-012-0862-6
7. Berzin V, Kiriukhin M, Tyurin M (2013) Selective n-butanol production by Clostridium sp. MTButOH1365 during continuous synthesis gas fermentation due to expression of synthetic thiolase, 3-hydroxy butyryl-CoA dehydrogenase, crotonase, butyryl-CoA dehydrogenase, butyraldehyde dehydrogenase and NAD-dependent butanol dehydrogenase. Appl Biochem Biotechnol 169(3):950-959. doi: 10.1007/s12010-012-0060-7
8. Cheung H, Tanke RS, Torrence GP (2005) "Acetic Acid". In: Ullmann's encyclopedia of industrial chemistry. Wiley-VCH, Weinheim. doi: 10.1002/14356007.a01-045
9. Csörgo B, Fehér T, Tímár E, Blattner FR, Pósfai G (2012) Low-mutation-rate, reduced-genome Escherichia coli: an improved host for faithful maintenance of engineered genetic constructs. Microb Cell Fact 11:11. doi: 10.1186/1475-2859-11-11
10. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97(12):6640-6645
11. Demain AL (2009) Biosolutions to the energy problem. J Ind Microbiol Biotechnol 36(3):319-332
12. Fischer CR, Klein-Marcuschamer D, Stephanopoulos G (2008) Selection and optimization of microbial hosts for biofuels production. Metab Eng 10(6):295-304
13. Gaddy JL, Arora DD, Ko C-W, Phillips JR, Basu R, Wikstrom CV, Clausen EC (2001) Methods for increasing the production of ethanol from microbial fermentation. US Patent 7285402
14. Global Industry Analysts, Inc. http://www.prweb.com/releases/formic-acid/ leather-textiles/prweb1569064.htm
15. Goldenstein C (2011) Algae Energy Costs. Coursework for PH240, Stanford University. http://large.stanford.edu/courses/2011/ph240/goldenstein1/.i
16. Köpke M, Michalcea C, Liew F-M, Tizard JH, Ali MS, Conolly JJ, Al-Sinawi B, Simpson SD (2011) 2, 3-Butanediol production by acetogenic bacteria, an alternative route to chemical synthesis, using industrial waste gas. Appl Environ Microbiol 77(15):5467-5475
17. Lee JH, Sung BH, Kim MS, Blattne r FR, Yoon BH, Kim JH, Kim SC (2009) Metabolic engineering of a reduced-genome strain of Escherichia coli for l-threonine production. Microb Cell Factories 8:2. doi: 10.1186/1475-2859-8-2
18. Munasinghe PC, Khanal SK (2010) Biomass-derived syngas fermentation into biofuels: opportunities and challenges. Bioresource Technol 101:5013-5022
19. Reutemann W, Kieczka H (2002) "Formic Acid". In: Ullmann's encyclopedia of industrial chemistry. Wiley-VCH, Weinheim. doi: 10.1002/14356007.a12-013
20. Robinson JM (2004) Lactones as new oxygenate fuel additives, fuels based thereon and methods for using same. US 20060096158Al
21. Ross SM (2000) Introduction to probability and statistics for engineers and scientists, 2nd edn. Academic Press, San Diego
22. Siddiqi MA, Rodwell VW (1967) Bacterial metabolism of mevalonic acid. J Bacteriol 93(1):207-214
23. Tanner RS, Miller LM, Yang D (1993) Clostridium ljungdahlii sp. nov., an acetogenic species in clostridial rRNA homology group I. Int J Systemic Bacteriol 43(2):232-236
24. Tyurin MV (1992) The method and apparatus for electric cell treatment using partial power capacitor discharge via the sample and the power tetrode. Russian Patent 2005776
25. Tyurin M, Lynd LR, Wiegel J (2006) Genetic delivery systems for strictly anaerobic thermophiles. In: Rainey, FA, Oren A (eds) Methods in microbiology, 1st edn, vol 35, pp 307-328. Elsevier, Academic Press, Amsterdam, Boston
26. Tyurin M, Kiriukhin M, Berzin V (2012) Electrofusion of cells of acetogen Clostridium sp. MT351 with erm(B) or cat in the chromosome. J Biotech Res 4:1-12
27. 2 blend fermentation. J Appl Microbiol. doi: 10.1111/jam.12123
28. Van Laar TA, Lin Y-H, Miller CL, Karna SLR, Chambers JP, Seshu J (2012) Effect of levels of acetate on the mevalonate pathway of Borrelia burgdorferi. PLoS ONE 7(5):e38171. doi: 10.1371/journal.pone.0038171
29. Van Leeuwen BNM, van der Wulp AM, Duijnstee I, van Maris AJA, Straathof AJJ (2012) Fermentative production of isobutene. Appl Microbiol Biotechnol 93(4):1377-1387. doi: 10.1007/s00253-011-3853-7
30. Volker M (2003) Energy conservation in acetogenic bacteria. Appl Envir Microbiol 69(11):6345-53. doi: 10.1128/AEM.69.11.6345-6353.2003
31. Yang J, Xian M, Su S, Zhao G, Nie Q, Jiang X, Zheng Y, Liu W (2012) Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli. PLoS ONE 7(4):e33509. doi: 10.1371/journal.pone.0033509
32. Young KD (2006) The selective value of bacterial shape. Microbiol Mol Biol Rev 70(3):660-703. doi: 10.1128/MMBR.00001-06