Acetogen biocatalyst Clostridium sp. MTETOH871 engineered with our proprietary electrotransformation technology and equipment: Continuous synthesis gas fermentation for selective ethanol production

Journal of Biotech Research

Volumn 4, Issue 1, 2012, Pages 54-64

  Berzin, Vel ^a   Tyurin, Michael ^a  

^a Syngas Biofuels Energy Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL VIABILITY; CLOSTRIDIUM LJUNGDAHLII; CO2 EMISSIONS; CONTINUOUS FERMENTATION; CONTINUOUS SYNTHESIS; ELECTROPERMEABILIZATION; ELECTROTRANSFORMATION; ETHANOL PRODUCTION; ETHANOL YIELD; EXPRESSION VECTORS; PHOSPHOTRANSACETYLASE GENES; PULSE CURRENTS; REAL TIME; SINGLE STAGE; SINGLE-STEP; SYN-GAS; SYNGAS FERMENTATIONS; TRANSFORMANTS;

ALDEHYDES; CARBON DIOXIDE; CELL MEMBRANES; CLONING; CLOSTRIDIUM; CYTOLOGY; ETHANOL; FERMENTATION; VOLATILE FATTY ACIDS;

SYNTHESIS GAS;

CLOSTRIDIUM; CLOSTRIDIUM LJUNGDAHLII; CLOSTRIDIUM SP.;

EID: 84869860325     PISSN: 19443285     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Demain AL. 2009. Biosolutions to the energy problem. J. Ind. Microbiol. Biotechnol. 36(3): 319-332
2. Köpke M, Michalcea C, Liew F-M, Tizard JH, Ali MS, Conolly JJ, Al-Sinawi B, Simpson SD. 2011. 2,3-Butanediol production by acetogenic bacteria, an alternative route to chemical synthesis, using industrial waste gas. Appl. Environ. Microbiol. 77(15): 5467-5475.
3. Fischer CR, Klein-Marcuschamer D, Stephanopoulos G. 2008. Selection and optimization of microbial hosts for biofuels production. Metab Eng. 10(6): 295-304.
4. Drake HL. 1994. (Ed.), Acetogenesis. Chapman & Hall. NY, London.
5. Gaddy JL, Arora DD, Ko C-W, Phillips JR, Basu R, Wikstrom CV, Clausen EC. 2001. Methods for increasing the production of ethanol from microbial fermentation. US Patent 7285402.
6. Köpke M, Held C, Hujer S, Liesegang H, Wiezer A, Wollherr A, Ehrenreich A, Lieblc W, Gottschalk G, Dürre P. 2010. Clostridium ljungdahlii represents a microbial production platform based on syngas. PNAS. 107 (29): 13087-13092.
7. Abubackar NH, Veiga MC, Kennes C. 2011. Biofuels, Bioproducts and Biorefining. 5(1): 93-114.
8. Paul D, Austin FW, Arick T, Bridges SM., Burgess, S.C., Dandass, Y.S., Lawrence, M.L., 2010. Genome Sequence of the Solvent-Producing Bacterium Clostridium carboxidivorans Strain P7. J. Bacteriol. 192 (20), 5554-5555.
9. Tyurin M, Kiriukhin M, Berzin V. 2012. Electrofusion of cells of Acetogen Clostridium sp. MT 351 with erm(B) or cat in the chromosome. Journal of Biotech Research. 4:13-24.
10. Tanner RS, Miller LM, Yang D. 1993. Clostridium ljungdahlii sp. nov., an Acetogenic Species in Clostridial rRNA Homology Group I. International Journal of Systematic Bacteriology. 43(2):232-236.
11. Bryksin AV, Matsumura I. 2010. Rational design of a plasmid origin that replicates efficiently in both Gram-positive and Gram-negative bacteria. PLos ONE. 5(10), e13244. doi:10.1371/journal.pone.0013244.
12. Tiurin MV. 1990. Optimization of the method of isolation of micro amounts of plasmid DNA from lactobacilli. Mol. Gen. Microbiol. Virusol. 3:29-31.
13. Tyurin MV. 1992. The method and apparatus for electric cell treatment using partial capacitor discharge via the sample and the power tetrode. Russian Patent 2-005776.
14. Tyurin MV, Sullivan CR, Lynd LR.. 2005. Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes. Appl. Envir. Microbiol. 71:8069-8076.
15. Shaw AJ, Desai SG, Lynd LR, Tyurin MV. 2010. Thermophilic organisms for conversion of lignocellulosic biomass to ethanol. US Application Publication No. 20100015678 A1.
16. Amador-Noguez D, Brasg IA, Feng XJ, Roque N, Rabinowitz JD. 2011. Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum. Appl. Environ. Microbiol. 77(22):7984-7997.