Expression analysis of efflux pump genes among drug-susceptible and multidrug-resistant Mycobacterium tuberculosis clinical isolates and reference strains

Diagnostic Microbiology and Infectious Disease

Volumn 76, Issue 3, 2013, Pages 291-297

  Calgin, Mustafa Kerem ^a   Sahin, Fikret ^a   Turegun, Buse ^a   Gerceker, Devran ^a   Atasever, Melike ^b   Koksal, Deniz ^b   Karasartova, Djursun ^a   Kiyan, Mehmet ^a  

^a ANKARA UNIVERSITY   (Turkey)

^b Ataturk Chest Disease and Thoracic Surgery Education and Research Hospital Ankara   (Turkey)

Author keywords

Drug resistance; Efflux pumps; Mycobacterium tuberculosis

Indexed keywords

ETHAMBUTOL; ISONIAZID; RIFAMPICIN; STREPTOMYCIN; TUBERCULOSTATIC AGENT;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRRA GENE; DRRB GENE; DRUG SENSITIVITY; GENE EXPRESSION; INIA GENE; MINIMUM INHIBITORY CONCENTRATION; MMPL4 GENE; MMPL7 GENE; MULTIDRUG EFFLUX PUMP GENE; MULTIDRUG RESISTANCE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; RV0037C GENE; RV0783C GENE; RV1250 GENE; RV1258C GENE; RV1273C GENE; RV1410C GENE; RV1634 GENE; RV1687C GENE; RV2333C GENE; RV3000 GENE; STRAIN DIFFERENCE;

ANTITUBERCULAR AGENTS; DRUG RESISTANCE, MULTIPLE, BACTERIAL; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENES, MDR; HUMANS; MICROBIAL SENSITIVITY TESTS; MYCOBACTERIUM TUBERCULOSIS; RIFAMPIN; STREPTOMYCIN; TUBERCULOSIS, MULTIDRUG-RESISTANT; TUBERCULOSIS, PULMONARY;

EID: 84879145597     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2013.02.033     Document Type: Article

References (21)

1. Brennan P.J. Structure, function, and biogenesis of the cell wall of Mycobacterium tuberculosis. Tuberculosis 2003, 83:91-97.
2. Choudhuri B.S., Bhakta S., Barik R., Basu J., Kundu M., Chakrabarti P. Overexpression and functional characterization of an ABC (ATP-binding cassette) transporter encoded by the genes drrA and drrB of Mycobacterium tuberculosis. Biochem J 2002, 367:279-285.
3. Colangeli R., Helb D., Sridharan S., Sun J., Varma-Basil M., Hazbon M.H., et al. The Mycobacterium tuberculosis iniA gene is essential for activity of an efflux pump that confers drug tolerance to both isoniazid and ethambutol. Mol Microbiol 2005, 55:1829-1840.
4. Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., et al. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 1998, 393:537-544.
5. De Rossi E., Ainsa J.A., Riccardi G. Role of mycobacterial efflux transporters in drug resistance: an unresolved question. FEMS Microbiol Rev 2006, 30:36-52.
6. Jiang X., Zhang W., Zhang Y., Gao F., Lu C., Zhang X., et al. Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR. Microb Drug Resist 2008, 14:7-11.
7. Levy S.B. Active efflux, a common mechanism for biocide and antibiotic resistance. Symp Ser Soc Appl Microbiol 2002, 31:65S-71S.
8. Li X.Z., Nikaido H. Efflux-mediated drug resistance in bacteria: an update. Drugs 2009, 69:1555-1623.
9. Lomovskaya O., Watkins W.J. Efflux pumps: their role in antibacterial drug discovery. Curr Med Chem 2001, 8:1699-1711.
10. Louw G.E., Warren R.M., Gey van Pittius N.C., McEvoy C.R., Van Helden P.D., Victor T.C. A balancing act: efflux/influx in mycobacterial drug resistance. Antimicrob Agents Chemother 2009, 53:3181-3189.
11. Marquez B. Bacterial efflux systems and efflux pumps inhibitors. Biochimie 2005, 87:1137-1147.
12. McMurry L., Petrucci R.E., Levy S.B. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. Proc Natl Acad Sci U S A 1980, 77:3974-3977.
13. Neyfakh A.A. Mystery of multidrug transporters: the answer can be simple. Mol Microbiol 2002, 44:1123-1130.
14. Niederweis M. Mycobacterial porins-new channel proteins in unique outer membranes. Mol Microbiol 2003, 49:1167-1177.
15. Nikaido H. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science 1994, 264:382-388.
16. Pasca M.R., Guglierame P., De Rossi E., Zara F., Riccardi G. mmpL7 gene of Mycobacterium tuberculosis is responsible for isoniazid efflux in Mycobacterium smegmatis. Antimicrob Agents Chemother 2005, 49:4775-4777.
17. Ramon-Garcia S., Martin C., De Rossi E., Ainsa J.A. Contribution of the Rv2333c efflux pump (the Stp protein) from Mycobacterium tuberculosis to intrinsic antibiotic resistance in Mycobacterium bovis BCG. J Antimicrob Chemother 2007, 59:544-547.
18. Ren Q., Chen K., Paulsen I.T. TransportDB: a comprehensive database resource for cytoplasmic membrane transport systems and outer membrane channels. Nucleic Acids Res 2007, 35:28.
19. Sharma S., Kumar M., Nargotra A., Koul S., Khan I.A. Piperine as an inhibitor of Rv1258c, a putative multidrug efflux pump of Mycobacterium tuberculosis. J Antimicrob Chemother 2010, 65:1694-1701.
20. Siddiqi N., Das R., Pathak N., Banerjee S., Ahmed N., Katoch V.M., et al. Mycobacterium tuberculosis isolate with a distinct genomic identity overexpresses a tap-like efflux pump. Infection 2004, 32:109-111.
21. Silva P.E., Bigi F., Santangelo M.P., Romano M.I., Martin C., Cataldi A., et al. Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis. Antimicrob Agents Chemother 2001, 45:800-804.