An improved method compatible with metagenomic analyses to extract genomic DNA from soils in Tuber melanosporum orchards

Journal of Applied Microbiology

Volumn 115, Issue 1, 2013, Pages 163-170

  Antony Babu, S ^a   Murat, C ^a   Deveau, A ^a   Le Tacon, F ^a   Frey Klett, P ^a   Uroz, S ^a  

^a UNIVERSITÉ DE LORRAINE   (France)

Author keywords

260 : 230 nm ratio; Calcareous soil; DNA extraction; EDTA; Guanidine thiocyanate; Soil; Tuber melanosporum

Indexed keywords

CHLORINE COMPOUNDS; DNA; ELECTROPHORESIS; EXTRACTION; GENES; MICROARRAYS; ORCHARDS; POLYCYCLIC AROMATIC HYDROCARBONS; POLYMERASE CHAIN REACTION; PROBLEM SOLVING; SOILS;

260 : 230 NM RATIO; CALCAREOUS SOILS; DNA EXTRACTION; GENOMIC DNA; GUANIDINE THIOCYANATE; HIGH-THROUGHPUT METHOD; METAGENOMIC ANALYSIS; PYROSEQUENCING; RE-EXTRACTION; TUBER MELANOSPORUM;

PHENOLS;

CHLOROFORM; GENOMIC DNA; PHENOL;

BACTERIUM; CALCAREOUS SOIL; CHLOROFORM; DNA; EDTA; ELECTROKINESIS; GENOMICS; MICROBIAL COMMUNITY; ORCHARD; PHENOL; SPECIES DIVERSITY;

ARTICLE; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; DNA PURIFICATION; HIGH THROUGHPUT SEQUENCING; METAGENOMICS; MICROARRAY ANALYSIS; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; NONHUMAN; ORCHARD; PYROSEQUENCING; SENSITIVITY AND SPECIFICITY; SOIL ANALYSIS; TUBER (GENUS); TUBER MELANOSPORUM;

BACTERIA (MICROORGANISMS); TUBER MELANOSPORUM;

EID: 84879073061     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12205     Document Type: Article

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