Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil

BMC Microbiology

Volumn 12, Issue , 2012, Pages

  Iotti, Mirco ^a   Leonardi, Marco ^b   Oddis, Marilena ^b   Salerni, Elena ^c   Baraldi, Elena ^a   Zambonelli, Alessandra ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF L'AQUILA   (Italy)

^c UNIVERSITY OF SIENA   (Italy)

Author keywords

ITS primers; Real time PCR; Soil DNA extraction; Taq man probe; Truffle production; Tuber magnatum DNA concentration

Indexed keywords

FUNGAL DNA; PRIMER DNA; RIBOSOME DNA;

ARTICLE; CONCENTRATION (PARAMETERS); DNA EXTRACTION; DNA PROBE; ECTOMYCORRHIZA; ENVIRONMENTAL FACTOR; FUNGAL DETECTION; MYCELIUM; MYCORRHIZA; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SOIL ANALYSIS; TUBER (GENUS); TUBER MAGNATUM; VALIDATION PROCESS;

ASCOMYCOTA; COLONY COUNT, MICROBIAL; DNA PRIMERS; DNA, FUNGAL; DNA, RIBOSOMAL SPACER; ITALY; OLIGONUCLEOTIDE PROBES; REAL-TIME POLYMERASE CHAIN REACTION; SOIL MICROBIOLOGY;

BASIDIOMYCOTA; TUBER MAGNATUM;

EID: 84861820909     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-12-93     Document Type: Article

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