Expression of alginases and alginate polymerase genes in response to oxygen, and their relationship with the alginate molecular weight in Azotobacter vinelandii

Enzyme and Microbial Technology

Volumn 53, Issue 2, 2013, Pages 85-91

  Flores, Celia ^a   Moreno, Soledad ^a   Espín, Guadalupe ^a   Peña, Carlos ^a   Galindo, Enrique ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

Alginase activity; Alginate polymerization; Azotobacter vinelandii; Dissolved oxygen; Transcription

Indexed keywords

AZOTOBACTER VINELANDII; DISSOLVED OXYGEN TENSION; HIGH MOLECULAR WEIGHT; PERIPLASMIC SPACE; POLYMER TRANSPORT; POLYMERASE COMPLEX; TRANSCRIPTION LEVEL; WILD-TYPE STRAIN;

ALGINATE; BIOSYNTHESIS; BLENDING; DISSOLVED OXYGEN; ENCODING (SYMBOLS); ENZYME ACTIVITY; GENES; MOLECULAR WEIGHT; POLYMERIZATION; POLYMERS; PROTEINS;

TRANSCRIPTION;

ALGINIC ACID; DISSOLVED OXYGEN; HYDROXYBUTYRIC ACID; OXYGEN;

ALG44 GENE; ALG8 GENE; ALGE7 GENE; ALGL GENE; ALYA1 GENE; ALYA2 GENE; ALYA3 GENE; ARTICLE; AZOTOBACTER VINELANDII; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION; MOLECULAR WEIGHT; NONHUMAN; OXYGEN TENSION; REAL TIME POLYMERASE CHAIN REACTION; STRUCTURAL GENE; SYNTHESIS;

ALGINATES; AZOTOBACTER VINELANDII; BACTERIAL PROTEINS; BIOMASS; GENE EXPRESSION REGULATION, BACTERIAL; INDUSTRIAL MICROBIOLOGY; MOLECULAR WEIGHT; OXYGEN; OXYGEN CONSUMPTION; POLYMERIZATION; POLYSACCHARIDE-LYASES; TRANSCRIPTION, GENETIC;

AZOTOBACTER VINELANDII;

EID: 84879023104     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2013.04.010     Document Type: Article

References (40)

1. Rehm B.H.A., Valla S. Bacterial alginates: biosynthesis and applications. Applied Microbiology and Biotechnology 1997, 48:281-288.
2. Rehm B.H.A. Alginate production: precursor biosynthesis, polymerization and secretion. Microbiology monographs. Alginates: biology and applications 2009, 55-71. Springer-Verlag Heidelberg, Berlin. B.H.A. Rehm (Ed.).
3. Galindo E., Peña C., Núñez C., Segura D., Espín G. Molecular and bioengineering strategies to improve alginate and polyhydroxyalkanoate production by Azotobacter vinelandii. Microbial Cell Factories 2007, 6:7.
4. Hernández R.M., Gorka O., Murua A., Pedraz J.L. Microcapsules and microcarriers for in situ cell delivery. Advanced Drug Delivery Reviews 2010, 62:711-730.
5. Lee K.Y., Mooney D.J. Alginate. Properties and biomedical applications. Progress in Polymer Science 2012, 37:106-126.
6. Parente E., Crudele M.A., Aquino M., Clementi F. Alginate production by Azotobacter vinelandii DSM576 in batch fermentation. Journal of Industrial Microbiology and Biotechnology 1998, 20:171-176.
7. Sabra W., Zeng A.-P., Sabry S., Omar S., Deckwer W.-D. Effect of phosphate and oxygen concentrations on alginate production and stoichiometry of metabolism of Azotobacter vinelandii under microaerobic conditions. Applied Microbiology and Biotechnology 1999, 52:773-780.
8. Parente E., Crudele M.A., Ricciardi A., Mancini M., Clementi F. Effect of ammonium sulphate concentration and agitation speed on the kinetics of alginate production by Azotobacter vinelandii DSM576 in batch fermentation. Journal of Industrial Microbiology and Biotechnology 2000, 125:242-248.
9. Peña C., Trujillo-Roldán M.A., Galindo E. Influence of dissolved oxygen tension and agitation speed on alginate production and its molecular weight in cultures of Azotobacter vinelandii. Enzyme and Microbial Technology 2000, 27:390-398.
10. Sabra W., Zeng A.-P., Lunsdorf H., Deckwer W.-D. Effect of oxygen on formation and structure of Azotobacter vinelandii alginate and its role in protecting nitrogenase. Applied and Environment Microbiology 2000, 66:4037-4044.
11. Trujillo-Roldán M.A., Moreno S., Espín G., Galindo E. The roles of oxygen and alginate-lyase in determining the molecular weight of alginate produced by Azotobacter vinelandii. Applied Microbiology and Biotechnology 2004, 63:742-747.
12. Remminghorst U., Rehm B.H.A. Bacterial alginates: from biosynthesis to applications. Biotechnology Letters 2006, 28:1701-1712.
13. Oglesby L.L., Jain S., Ohman D.E. Membrane topology and roles of Pseudomonas aeruginosa Alg8 and Alg44 in alginate polymerization. Microbiology 2008, 154:1605-1615.
14. Remminghorst U., Rehm B.H.A. In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa. Applied and Environment Microbiology 2006, 72:298-305.
15. Remminghorst U., Hay I.D., Rehm B.H.A. Molecular characterization of Alg8, a putative glycosyltransferase, involved in alginate polymerisation. Journal of Biotechnology 2009, 140:176-183.
16. Remminghorst U., Rehm B.H.A. Alg44, a unique protein required for alginate biosynthesis in Pseudomonas aeruginosa. FEBS Letters 2006, 580:3883-3888.
17. Setubal J.C., dos Santos P., Goldman B.S., Ertesvåg H., Espín G., Rubio L.M., et al. Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes. Journal of Bacteriology 2009, 191:4534-4545.
18. Gimmestad M., Ertesvåg H., Heggeset T.M., Aarstad O., Svanem B.I., Valla S. Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination. Journal of Bacteriology 2009, 191:4845-4853.
19. Merighi M., Lee V.T., Hyodo M., Hayakawa Y., Lory S. The second messenger bis-(3'-5')-cyclic-GMP and its PilZ domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa. Molecular Microbiology 2007, 65:876-895.
20. Akama H., Matsuura T., Kashiwagi S., Yoneyama H., Narita S.-I., Tsukihara T., Nakagawa A., Nakae T. Crystal structure of the membrane fusion protein, MexA, of the multidrug transporter in Pseudomonas aeruginosa. Journal of Biological Chemistry 2004, 79:25939-25942.
21. Díaz-Barrera A., Aguirre A., Berrios J., Acevedo F. Continuous cultures for alginate production by Azotobacter vinelandii growing at different oxygen uptake rates. Process Biochemistry 2011, 46:1879-1883.
22. Díaz-Barrera A., Soto E., Altamirano C. Alginate production and alg8 expression by Azotobacter vinelandii in continuous cultures. Journal of Industrial Microbiology and Biotechnology 2012, 39:613-621.
23. Leitão J.H., Sá-Correia I. Oxygen-dependent upregulation of transcription of alginate genes algA, algC and algD in Pseudomonas aeruginosa. Research in Microbiology 1997, 148:37-43.
24. Horan N.J., Jarman T.R., Dawes E.A. Studies on some enzymes of alginic acid biosynthesis in Azotobacter vinelandii grown in continuous culture. Journal of General Microbiology 1983, 129:2985-2990.
25. Lozano E., Galindo E., Peña C. Oxygen transfer rate during the production of alginate by Azotobacter vinelandii under oxygen-limited and non oxygen-limited conditions. Microbial Cell Factories 2011, 10:13.
26. Lowry O.H., Rosenbrough N.J., Lewis A., Farr S., Randall R.J. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 1951, 193:265-275.
27. Karr D.B., Waters J.W., Emerich D.W. Analysis of poly-β-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Applied and Environment Microbiology 1983, 46:1339-1344.
28. Miller G. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 1959, 31:426-428.
29. Trujillo-Roldán M.A., Moreno S., Segura D., Galindo E., Espín G. Alginate production by an Azotobacter vinelandii mutant unable to produce alginate lyase. Applied Microbiology and Biotechnology 2003, 60:733-737.
30. Ertesvåg H., Erlien F., Skjåk-Bræk G., Rehm B.H.A., Valla S. Biochemical properties and substrate specificities of a recombinantly produced Azotobacter vinelandii alginate lyase. Journal of Bacteriology 1998, 180:3779-3784.
31. Franklin M.J., Ohman D.E. Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa. Journal of Bacteriology 2002, 184:3000-3007.
32. Ntr are involved in regulation of the poly-β-hydroxybutyrate biosynthetic genes in Azotobacter vinelandii. Journal of Molecular Microbiology and Biotechnology 2008, 15:244-254.
33. Barry T., Geary S., Hannify S., MacGearailt C., Shalloo M., Heery D., et al. Rapid mini-preparations of total RNA from bacteria. Nucleic Acids Research 1992, 20:4940.
34. -δδCT method. Methods 2001, 25:402-408.
35. Klimek J., Ollis D. Extracellular microbial polysaccharides: kinetics of Pseudomonas sp. Azotobacter vinelandii, and Aureobasidium pullulans batch fermentations. Biotechnology and Bioengineering 1980, 22:2321-2342.
36. Robles-Price A., Wong T.Y., Sletta H., Valla S., Schiller N.L. AlgX is a periplasmic protein required for alginate biosynthesis in Pseudomonas aeruginosa. Journal of Bacteriology 2004, 186:7369-7377.
37. Jain S., Ohman D.E. Role of an alginate lyase for alginate transport in mucoid Pseudomonas aeruginosa. Infection and Immunity 2005, 73:6429-6436.
38. Bakkevig K., Sletta H., Gimmestad M., Aune R., Ertesvåg H., Degnes K., et al. Role of the Pseudomonas fluorescens alginate lyase (AlgL) in clearing the periplasm of alginates not exported to the extracellular environment. Journal of Bacteriology 2005, 187:8375-8384.
39. Hay I.D., Remminghorst U., Rehm B.H. MucR, a novel membrane-associated regulator of alginate biosynthesis in Pseudomonas aeruginosa. Applied and Environment Microbiology 2009, 75:1110-1120.
40. Manzo J., Cocotl-Yañez M., Tzontecomani T., Martínez V.M., Bustillos R., Velásquez C., et al. Post-transcriptional regulation of the alginate biosynthetic gene algD by the Gac/Rsm system in Azotobacter vinelandii. Journal of Molecular Microbiology and Biotechnology 2011, 21:147-159.