Membrane topology and roles of Pseudomonas aeruginosa Alg8 and Alg44 in alginate polymerization

Microbiology

Volumn 154, Issue 6, 2008, Pages 1605-1615

  Oglesby, Lashanda L ^a,b   Jain, Sumita ^a,b,c   Ohman, Dennis E ^a,b  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

^c UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ALGINIC ACID; ALKALINE PHOSPHATASE; AMPICILLIN; CARBENICILLIN; GENTAMICIN; GLYCOSYLTRANSFERASE; HYBRID PROTEIN; KANAMYCIN; MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN; PORIN; SIGNAL PEPTIDE; TETRACYCLINE; ALG44 PROTEIN, PSEUDOMONAS AERUGINOSA; ALG8 PROTEIN, PSEUDOMONAS AERUGINOSA; BACTERIAL PROTEIN; GLUCURONIC ACID; HEXURONIC ACID; POLYMER;

AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ARTICLE; CARBOHYDRATE SYNTHESIS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; DRUG TRANSPORT; ENZYME ACTIVE SITE; ENZYME ASSAY; IN VIVO STUDY; MEMBRANE STRUCTURE; MUTAGENESIS; NONHUMAN; POLYMERIZATION; PREDICTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN MOTIF; PROTEIN STABILITY; PSEUDOMONAS AERUGINOSA; SITE DIRECTED MUTAGENESIS; WESTERN BLOTTING; CELL MEMBRANE; ESCHERICHIA COLI; GENETICS; METABOLISM; SEQUENCE HOMOLOGY;

PSEUDOMONAS AERUGINOSA;

ALGINATES; ALKALINE PHOSPHATASE; BACTERIAL PROTEINS; CELL MEMBRANE; ESCHERICHIA COLI; GLUCURONIC ACID; HEXURONIC ACIDS; MEMBRANE PROTEINS; POLYMERS; PORINS; PSEUDOMONAS AERUGINOSA; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 48449097813     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.2007/015305-0     Document Type: Article

References (43)

1. Barny, M. A., Schoonejans, E., Economou, A., Johnston, A. W. & Downie, J. A. (1996). The C-terminal domain of the Rhizobium leguminosarum chitin synthase NodC is important for function and determines the orientation of the N-terminal region in the inner membrane. Mol Microbiol 19, 443-453.
2. Boucher, J. C., Martinez-Salazar, J., Schurr, M. J., Mudd, M. H., Yu, H. & Deretic, V. (1996). Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA. J Bacteriol 178, 511-523.
3. Brickman, E. & Beckwith, J. (1975). Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages. J Mol Biol 96, 307-316.
4. Campbell, J. A., Davies, G. J., Bulone, V. & Henrissat B. (1997). A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem J 326, 929-939.
5. DeVries, C. A. & Ohman, D. E. (1994). Mucoid to nonmucoid conversion in alginate-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT, encoding a putative alternative sigma factor, and shows evidence for autoregulation. J Bacteriol 176, 6677-6687.
6. Elkins, C. A. & Nikaido, H. (2003). Chimeric analysis of AcrA function reveals the importance of its C-terminal domain in its interaction with the AcrB multidrug efflux pump. J Bacteriol 185, 5349-5356.
7. Forsee, W. T., Cartee, R. T. & Yother, J. (2000). Biosynthesis of type 3 capsular polysaccharide in Streptococcus pneumoniae. Enzymatic chain release by an abortive translocation process. J Biol Chem 275, 25972-25978.
8. Franklin, M. J. & Ohman, D. E. (2002). Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa. J Bacteriol 184, 3000-3007.
9. Franklin, M. J., Chitnis, C. E., Gacesa, P., Sonesson, A., White, D. C. & Ohman, D. E. (1994). Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase. J Bacteriol 176, 1821-1830.
10. Garinot-Schneider, C., Lellouch, A. C. & Geremia, R. A. (2000). Identification of essential amino acid residues in the Sinorhizobium meliloti glucosyltransferase ExoM. J Biol Chem 275, 31407-31413.
11. Gilligan, P. H. (1991). Microbiology of airway disease in patients with cystic fibrosis. Clin Microbiol Rev 4, 35-51.
12. Gutierrez, C. & Devedjian, J. C. (1989). A plasmid facilitating in vitro construction of phoA gene fusions in Escherichia coli. Nucleic Acids Res 17, 3999.
13. Haardt, M. & Bremer, E. (1996). Use of phoA and lacZ fusions to study the membrane topology of ProW, a component of the osmoregulated ProU transport system of Escherichia coli. J Bacteriol 178, 5370-5381.
14. Jain, S. & Ohman, D. E. (1998). Deletion of algK in mucoid Pseudomonas aeruginosa blocks alginate polymer formation and results in uronic acid secretion. J Bacteriol 180, 634-641.
15. Jain, S. & Ohman, D. E. (2004). Alginate biosynthesis. In Pseudomonas, pp. 53-81. Edited by J.-L. Ramos. New York: Kluwer Academic/Plenum Publishers.
16. Jain, S. & Ohman, D. E. (2005). Role of an alginate lyase for alginate transport in mucoid Pseudomonas aeruginosa. Infect Immun 73, 6429-6436.
17. Jain, &, Franklin, M. J., Ertesvag, H., Valla, S. & Ohman, D. E. (2003). The dual roles of AlgG in C-5-epimerization and secretion of alginate polymers in Pseudomonas aeruginosa. Mol Microbiol 47, 1123-1133.
18. Johnson, J. M. & Church, G. M. (1999). Alignment and structure prediction of divergent protein families: periplasmic and outer membrane proteins of bacterial efflux pumps. J Mol Biol 287, 695-715.
19. Keenleyside, W. J., Clarke, A. J. & Whitfield, C. (2001). Identification of residues involved in catalytic activity of the inverting glycosyl transferase WbbE from Salmonella enterica serovar borreze. J Bacteriol 183, 77-85.
20. Knutson, C. A. & Jeanes, A. (1968). A new modification of the carbazole analysis: application to heteropolysaccharides. Anal Biochem 24, 470-481.
21. Lobsanov, Y. D., Romero, P. A., Sleno, B, Yu, B., Yip, P., Herscovics, A. & Howell, P. L. (2004). Structure of Kre2p/Mnt1p: a yeast α1,2-mannosyltransferase involved in mannoprotein biosynthesis. J Biol Chem 279, 17921-17931.
22. Maharaj, R., May, T. B., Wang, S. K. & Chakrabarty, A. M. (1993). Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa. Gene 136, 267-269.
23. Manoil, C., Boyd, D. & Beckwith, J. (1988). Molecular genetic analysis of membrane protein topology. Trends Genet 4, 223-226.
24. Mejia-Ruiz, H., Guzman, J., Moreno, S., Soberon-Chavez, G. & Espin, G. (1997). The Azotobacter vinelandii alg8 and alg44 genes are essential for alginate synthesis and can be transcribed from an algD-independent promoter. Gene 199, 271-277.
25. Merighi, M., Lee, V. T., Hyodo, M., Hayakawa, Y. & Lory, S. (2007). The second messenger bis-(3′-5′)-cyclic-GMP and its Pilz domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa. Mol Microbiol 65, 876-895.
26. Nehme, D., Li, X. Z., Elliot R. & Poole, K. (2004). Assembly of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa: identification and characterization of mutations in mexA compromising MexA multimerization and interaction with MexB. J Bacteriol 186, 2973-2983.
27. Ohman, D. E. & Chakrabarty, A. M. (1981). Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate. Infect Immun 33, 142-148.
28. Pier, G. B., Coleman, F., Grout M., Franklin, M. & Ohman, D. E. (2001). Role of alginate O acetylation in resistance of mucoid Pseudomonas aeruginosa to opsonic phagocytosis. Infect Immun 69, 1895-1901.
29. Pugsley, A. P. (1989). Early stages in the secretory pathway. In Protein Targeting, chapter III, pp. 45-111. Edited by A. P. Pugsley. San Diego: Academic Press.
30. Rehm, B. H., Boheim, G., Tommassen, J. & Winkler, U. K. (1994). Overexpression of algE in Escherichia coli: subcellular localization, purification, and ion channel properties. J Bacteriol 176, 5639-5647.
31. Remminghorst, U. & Rehm, B. H. (2006a). In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa. Appl Environ Microbiol 72, 298-305.
32. Remminghorst, U. & Rehm, B. H. (2006b). Alg44, a unique protein required for alginate biosynthesis in Pseudomonas aeruginosa. FEBS Lett 580, 3883-3888.
33. Robles-Price, A., Wong, T. Y., Sletta, H, Valla, S. & Schiller, N. L. (2004). AlgX is a periplasmic protein required for alginate biosynthesis in Pseudomonas aeruginosa. J Bacteriol 186, 7369-7377.
34. Romling, U., Gomelsky, M. & Galperin, M. Y. (2005). C-di-GMP: the dawning of a novel bacterial signalling system. Mol Microbiol 57, 629-639.
35. Roychoudhury, S., May, T., Gill, J., Singh, S, Feingold, D. & Chakrabarty, A. (1989). Purification and characterization of guanosine diphospho-D-mannose dehydrogenase. A key enzyme in the biosynthesis of alginate by Pseudomonas aeruginosa. J Biol Chem 264, 9380-9385.
36. Saxena, I. M. & Brown, R. M., Jr (1997). Identification of cellulose synthase(s) in higher plants: sequence analysis of processive β-glycosyltransferases with the common motif 'D,D,D35Q(R,Q)XRW'. Cellulose 4, 33-49.
37. Saxena, I. M., Lin, F. C. & Brown, R. M., Jr (1990). Cloning and sequencing of the cellulose synthase catalytic subunit gene of Acetobacter xylinum. Plant Mol Biol 15, 673-683.
38. Saxena, I. M., Brown, R. M., Fevre, M., Geremia, R. A. & Henrissat B. (1995). Multidomain architecture of β-glycosyl transferases: implications for mechanism of action. J Bacteriol 177, 1419-1424.
39. Saxena, I. M., Brown, R. M., Jr & Dandekar, T. (2001). Structure-function characterization of cellulose synthase: relationship to other glycosyltransferases. Phytochemistry 57, 1135-1148.
40. Schiller, N. L., Monday, S. R., Boyd, C. M., Keen, N. T. & Ohman, D. E. (1993). Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli. J Bacteriol 175, 4780-4789.
41. Schweizer, H. P. (1992). Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol Microbiol 6, 1195-1204.
42. Shinabarger, D., Berry, A., May, T. B., Rothmell, R., Fialho, A. & Chakrabarty, A. M. (1991). Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase - a bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa. J Biol Chem 266, 2080-2088.
43. Tikhonova, E. B., Wang, Q. & Zgurskaya, H. I. (2002). Chimeric analysis of the multicomponent multidrug efflux transporters from gram-negative bacteria. J Bacteriol 184, 6499-6507.