Role of the Pseudomonas fluorescens alginate lyase (AlgL) in clearing the periplasm of alginates not exported to the extracellular environment

Journal of Bacteriology

Volumn 187, Issue 24, 2005, Pages 8375-8384

  Bakkevig, Karianne ^a   Sletta, Håvard ^b   Gimmestad, Martin ^a   Aune, Randi ^b   Ertesvåg, Helga ^a   Degnes, Kristin ^a   Christensen, Bjørn Erik ^a   Ellingsen, Trond E ^b   Valla, Svein ^a  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^b SINTEF MATERIALS AND CHEMISTRY   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

ALGINATE LYASE; ALGINIC ACID; BACTERIAL ENZYME; DNA; LYASE; POLYMER; POLYSACCHARIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; BACTERIUM MUTANT; BIODEGRADATION; COLONY FORMING UNIT; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE MUTATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; TRANSPOSON;

ALGINATES; CARBON DIOXIDE; COLONY COUNT, MICROBIAL; GENE DELETION; GLUCURONIC ACID; HEXURONIC ACIDS; MODELS, BIOLOGICAL; MUTAGENESIS, INSERTIONAL; PERIPLASM; POLYSACCHARIDE-LYASES; PSEUDOMONAS FLUORESCENS;

AZOTOBACTER; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 28844451839     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.187.24.8375-8384.2005     Document Type: Article

References (42)

1. Albrecht, M. T., and N. L. Schiller. 2005. Alginate lyase (AlgL) activity is required for alginate biosynthesis in Pseudomonas aeruginosa. J. Bacteriol. 187:3869-3872.
2. Bjerkan, T. M., C. L. Bender, H. Ertesvåg, F. Drabløs, M. K. Fakhr, L. A. Preston, G. Skjåk-Bræk, and S. Valla. 2004. The Pseudomonas syringae genome encodes a combined mannuronan C-5-epimerase and O-acetyl hydrolase, which strongly enhances the predicted gel-forming properties of alginates. J. Biol. Chem. 279:28920-28929.
3. Blatny, J. M., T. Brautaset, H. C. Winther-Larsen, K. Haugan, and S. Valla. 1997. Construction and use of a versatile set of broad-host-range cloning and expression vectors based on the RK2 replicon. J. Bacteriol. 63:370-379.
4. Boyd, A., M. Ghosh, T. B. May, D. Shinabarger, R. Keogh, and A. M. Chakrabarty. 1993. Sequence of the algL gene of Pseudomonas aeruginosa and purification of its alginate lyase product. Gene 131:1-8.
5. Chitnis, C. E., and D. E. Ohman. 1993. Genetic analysis of the alginate biosynthetic gene cluster of Pseudomonas aeruginosa shows evidence of an operonic structure. Mol. Microbiol. 8:583-593.
6. Christensen, B. E., A. S. Ulset, M. U. Beer, B. E. Knuckles, D. L. Williams, M. L. Fishman, H. K. Chau, and P. J. Wood. 2001. Macromolecular characterisation of three barley beta-glucan standards by size-exclusion chromatography combined with light scattering and viscometry: an inter-laboratory study. Carbohydr. Polymers 45:11-22.
7. de Lorenzo, V., I. Cases, M. Herrero, and K. N. Timmis. 1993. Early and late responses of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters. J. Bacteriol. 175:6902-6907.
8. Ertesvåg, H., B. Doseth, B. Larsen, G. Skjåk-Bræk, and S. Valla. 1994. Cloning and expression of an Azotobacter vinelandii mannuronan C-5-epimerase gene. J. Bacteriol. 176:2846-2853.
9. Ertesvåg, H., F. Erlien, G. Skjåk-Bræk, B. H. Rehm, and S. Valla. 1998. Biochemical properties and substrate specificities of a recombinantly produced Azotobacter vinelandii alginate lyase. J. Bacteriol. 180:3779-3784.
10. Ertesvåg, H., H. K. Høidal, I. K. Hals, A. Rian, B. Doseth, and S. Valla. 1995. A family of modular type mannuronan C-5-epimerase genes controls alginate structure in Azotobacter vinelandii. Mol. Microbiol. 16:719-731.
11. Ertesvåg, H., and S. Valla. 1998. Biosynthesis and applications of alginates. Polymer Degrad. Stabil. 59:85-91.
12. Ertesvåg, H., and G. Skjåk-Bræk. 1999. Modification of alginate using mannuronan C-5-epimerases, p. 71-78. C. Bucke (ed.), Methods in biotechnology 10: carbohydrate bio/technology protocols. Humana Press, Inc., Totowa, N.J.
13. Franklin, M. J., C. E. Chitnis, P. Gacesa, A. Sonesson, D. C. White, and D. E. Ohman. 1994. Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase. J. Bacteriol. 176:1821-1830.
14. Franklin, M. J., and D. E. Ohman. 2002. Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa. J. Bacteriol. 184:3000-3007.
15. Gacesa, P. 1998. Bacterial alginate biosynthesis-recent progress and future prospects. Microbiology 144:1133-1143.
16. Gaona, G., C. Nuñez, J. B. Goldberg, A. S. Linford, R. Najera, M. Castañeda, J. Guzman, G. Espin, and G. Soberon-Chavez. 2004. Characterization of the Azotobacter vinelandii algC gene involved in alginate and lipopolysaccharide production. FEMS Microbiol. Lett. 238:199-206.
17. Gill, J. F., V. Deretic, and A. M. Chakrabarty. 1986. Overproduction and assay of Pseudomonas aeruginosa phosphomannose isomerase. J. Bacteriol. 167:611-615.
18. Gimmestad, M., H. Sletta, H. Ertesvåg, K. Bakkevig, S. Jain, S. J. Suh, G. Skjåk-Bræk, T. E. Ellingsen, D. E. Ohman, and S. Valla. 2003. The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation. J. Bacteriol. 185:3515-3523.
19. Gimmestad, M., H. Sletta, P. Karunakaran, K. Bakkevig, H. Ertesvåg, T. Ellingsen, G. Skjåk-Bræk, and S. Valla. February 2004. Patent WO 2004/011628 A1. New mutant strains of Pseudomonas fluorescens and variants thereof, methods for their production, and uses thereof in alginate production.
20. Govan, J. R., J. A. M. Fyfe, and C. McMillan. 1979. The instability of mucoid Pseudomonas aeruginosa: fluctuation test and improved stability of the mucoid form in shaken culture. J. Gen. Microbiol. 110:229-232.
21. Govan, J. R., and J. W. Nelson. 1992. Microbiology of lung infection in cystic fibrosis. Br. Med. Bull. 48:912-930.
22. Grasdalen, H. 1983. High-field, H-1-NMR spectroscopy of alginate: sequential structure and linkage conformations. Carbohydr. Res. 118:255-260.
23. Jain, S., M. J. Franklin, H. Ertesvåg, S. Valla, and D. E. Ohman. 2003. The dual roles of AlgG in C-5-epimerization and secretion of alginate polymers in Pseudomonas aeruginosa. Mol. Microbiol. 47:1123-1133.
24. Jain, S., and D. E. Ohman. 1998. Deletion of algK in mucoid Pseudomonas aeruginosa blocks alginate polymer formation and results in uronic acid secretion. J. Bacteriol. 180:634-641.
25. Martin, D. W., M. J. Schurr, M. H. Mudd, J. R. W. Govan, B. W. Holloway, and V. Deretic. 1993. Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients. Proc. Natl. Acad. Sci. USA 90:8377-8381.
26. Monday, S. R., and N. L. Schiller. 1996. Alginate synthesis in Pseudomonas aeruginosa: the role of AlgL (alginate lyase) and AlgX. J. Bacteriol. 178:625-632.
27. 26a. Østgaard, K. 1992. Enzymatic microassay for the determination and characterization of alginates. Carhohydr. Polymers 19:51-59.
28. Pecina, A., A. Pascual, and A. Paneque. 1999. Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme. J. Bacteriol. 181:1409-1414.
29. Preston, L. A., C. L. Bender, and N. L. Schiller. 2001. Analysis and expression of algL, which encodes alginate lyase in Pseudomonas syringae pv. syringae. DNA Sequence 12:455-461.
30. Rehm, B. H., H. Ertesvåg, and S. Valla. 1996. A new Azotobacter vinelandii mannuronan C-5-epimerase gene (algG) is part of an alg gene cluster physically organized in a manner similar to that in Pseudomonas aeruginosa. J. Bacteriol. 178:5884-5889.
31. Robles-Price, A., T. Y. Wong, H. Sletta, S. Valla, and N. L. Schiller. 2004. AlgX is a periplasmic protein required for alginate biosynthesis in Pseudomonas aeruginosa. J. Bacteriol. 186:7369-7377.
32. Sambrook, J., and D. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
33. Schiller, N. L., S. R. Monday, C. M. Boyd, N. T. Keen, and D. E. Ohman. 1993. Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli. J. Bacteriol. 175:4780-4789.
34. Simon, R., U. Priefer, and A. Pühler. 1983. A broad host range mobilization system for in vivo engineering: transposon mutagenesis in gram negative bacteria. Biotechnology (New York) 1:784-791.
35. Skjåk-Bræk, G., H. Grasdalen, and B. Larsen. 1986. Monomer sequence and acetylation pattern in some bacterial alginates. Carbohydr. Res. 154:239-250.
36. Sletta, H., A. Nedal, T. E. Aune, H. Hellebust, S. Hakvåg, R. Aune, T. E. Ellingsen, S. Valla, and T. Brautaset. 2004. Broad-host-range plasmid pJB658 can be used for industrial-level production of a secreted host-toxic single-chain antibody fragment in Escherichia coli. Appl. Environ. Microbiol. 70:7033-7039.
37. Svanem, B. I., G. Skjåk-Bræk, H. Ertesvåg, and S. Valla. 1999. Cloning and expression of three new Azotobacter vinelandii genes closely related to a previously described gene family encoding mannuronan C-5-epimerases. J. Bacteriol. 181:68-77.
38. Trujillo-Roldan, M. A., S. Moreno, D. Segura, E. Galindo, and G. Espin. 2003. Alginate production by an Azotobacter vinelandii mutant unable to produce alginate lyase. Appl. Microbiol. Biotechnol. 60:733-737.
39. Valla, S., J. Li, H. Ertesvåg, T. Barbeyron, and U. Lindahl. 2001. Hexuronyl C5-epimerases in alginate and glycosaminoglycan biosynthesis. Biochimie 83:819-830.
40. Vazquez, A., S. Moreno, J. Guzman, A. Alvarado, and G. Espin. 1999. Transcriptional organization of the Azotobacter vinelandii algGXLVIFA genes: characterization of algF mutants. Gene 232:217-222.
41. Winther-Larsen, H. C., K. D. Josefsen, T. Brautaset, and S. Valla. 2000. Parameters affecting gene expression from the Pm promoter in Gram-negative bacteria. Metab. Eng. 2:79-91.
42. Zielinski, N. A., A. M. Chakrabarty, and A. Berry. 1991. Characterization and regulation of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase. J. Biol. Chem. 266:9754-9763.