Ethanol alters proliferation and differentiation of normal and chromosomally abnormal human embryonic stem cell-derived neurospheres

Birth Defects Research Part B - Developmental and Reproductive Toxicology

Volumn 98, Issue 3, 2013, Pages 283-295

  Krishnamoorthy, Malini ^a,h   Gerwe, Brian A ^e,g   Scharer, Christopher D ^a   Sahasranaman, Vanita ^b   Eilertson, Carmen D ^c   Nash, Rachel J ^d,h   Usta, Sümeyra Naz ^h   Kelly, Shasmine ^c   Rose, Matthew ^c   Peraza, Rene ^c   Arumugham, Jagan ^h   Stewart, Bethany ^a   Stice, Steven L ^f   Nash, Rodney J ^a,c,h  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^c GEORGIA STATE UNIVERSITY   (United States)

^d EMORY UNIVERSITY   (United States)

^e UNIVERSITY OF GEORGIA   (United States)

^f UNIVERSITY OF GEORGIA   (United States)

^g ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^h Jeevan Biosciences INC   (United States)

Author keywords

Differentiation; Fetal alcohol spectrum disorder; Gene expression; HESC; Methylation; Neurospheres

Indexed keywords

ALCOHOL; DNA;

ARTICLE; CELL CYCLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CHROMOSOME ABERRATION; CONTROLLED STUDY; DNA METHYLATION; EMBRYONIC STEM CELL; EXPOSURE; GENE EXPRESSION; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

BROMODEOXYURIDINE; CELL COUNT; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CELL SHAPE; CHROMOSOME ABERRATIONS; DNA METHYLATION; EMBRYONIC STEM CELLS; ETHANOL; G2 PHASE; GENE EXPRESSION REGULATION; HUMANS; INDOLES; MITOSIS; NEURONS; RNA, MESSENGER; SPHEROIDS, CELLULAR;

EID: 84878993098     PISSN: 15429733     EISSN: 15429741     Source Type: Journal    
DOI: 10.1002/bdrb.21063     Document Type: Article

References (39)

1. Anthony B, Zhou FC, Ogawa T, Goodlett CR, Ruiz J. 2008. Alcohol exposure alters cell cycle and apoptotic events during early neurulation. Alcohol Alcohol 43(3):261-273.
2. Baylin SB. 1997. Tying it all together: epigenetics, genetics, cell cycle, and cancer. Science 277(5334):1948-1949.
3. Bignami A, Eng LF, Dahl D, Uyeda CT. 1972. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res 43(2):429-435.
4. Braza-Boils A, Tomas M, Marin MP, Megias L, Sancho-Tello M, Fornas E, Renau-Piqueras J. 2006. Glycosylation is altered by ethanol in rat hippocampal cultured neurons. Alcohol Alcohol 41(5):494-504.
5. Burd L, Martsolf JT. 1989. Fetal alcohol syndrome: diagnosis and syndromal variability. Physiol Behav 46(1):39-43.
6. Camarillo C, Miranda RC. 2008. Ethanol exposure during neurogenesis induces persistent effects on neural maturation: evidence from an ex vivo model of fetal cerebral cortical neuroepithelial progenitor maturation. Gene Expr 14(3):159-171.
7. Coles CD, Kable JA, Drews-Botsch C, Falek A. 2000. Early identification of risk for effects of prenatal alcohol exposure. J Stud Alcohol 61(4):607-616.
8. Dhara SK, Hasneen K, Machacek DW, Boyd NL, Rao RR, Stice SL. 2008. Human neural progenitor cells derived from embryonic stem cells in feeder-free cultures. Differentiation 76(5):454-464.
9. Dhara SK, Stice SL. 2008. Neural differentiation of human embryonic stem cells. J Cell Biochem 105(3):633-640.
10. Dutra MF, Jaeger M, Ilha J, Kalil-Gaspar PI, Marcuzzo S, Achaval M. 2012. Exercise improves motor deficits and alters striatal GFAP expression in a 6-OHDA-induced rat model of Parkinson's disease. Neurol Sci Oct;33(5):1137-1144.
11. Garcia AD, Doan NB, Imura T, Bush TG, Sofroniew MV. 2004. GFAP-expressing progenitors are the principal source of constitutive neurogenesis in adult mouse forebrain. Nat Neurosci 7(11):1233-1241.
12. Garro AJ, McBeth DL, Lima V, Lieber CS. 1991. Ethanol consumption inhibits fetal DNA methylation in mice: implications for the fetal alcohol syndrome. Alcohol Clin Exp Res 15(3):395-398.
13. Gerwe BA, Angel PM, West FD, Hasneen K, Young A, Orlando R, Stice SL. 2011. Membrane proteomic signatures of karyotypically normal and abnormal human embryonic stem cell lines and derivatives. Proteomics 11(12):2515-2527.
14. Hicks SD, Middleton FA, Miller MW. 2010. Ethanol-induced methylation of cell cycle genes in neural stem cells. J Neurochem 114(6):1767-1780.
15. Ikonomidou C, Bittigau P, Ishimaru MJ, Wozniak DF, Koch C, Genz K, Price MT, Stefovska V, Horster F, Tenkova T, Dikranian K, Olney JW. 2000. Ethanol-induced apoptotic neurodegeneration and fetal alcohol syndrome. Science 287(5455):1056-1060.
16. Jones KL, Smith DW. 1973. Recognition of the fetal alcohol syndrome in early infancy. Lancet 302(7836):999-1001.
17. Jones KL, Smith DW, Ulleland CN, Streissguth P. 1973. Pattern of malformation in offspring of chronic alcoholic mothers. Lancet 1(7815):1267-1271.
18. Kahkonen S, Kesaniemi M, Nikouline VV, Karhu J, Ollikainen M, Holi M, Ilmoniemi RJ. 2001. Ethanol modulates cortical activity: direct evidence with combined TMS and EEG. Neuroimage 14(2):322-328.
19. Khavari DA, Sen GL, Rinn JL. 2010. DNA methylation and epigenetic control of cellular differentiation. Cell Cycle 9(19):3880-3883.
20. Krishnamoorthy M, Gerwe BA, Heimburg-Molinaro J, Nash RJ, Arumugham J, Eilertson CD, Stice SL, Nash RJ. 2010a. Ethanol alters cell cycle gene expression in human embryonic stem cells. J Pediatr Biochem 1(3):201-208.
21. Krishnamoorthy M, Gerwe BA, Scharer CD, Heimburg-Molinaro J, Gregory F, Nash RJ, Arumugham J, Stewart B, Stice SL. 2010b. Low ethanol concentration alters CHRNA5 RNA levels during early human development. Reprod Toxicol 30(3):489-492.
22. Krishnamoorthy M, Gerwe BA, Scharer CD, Heimburg-Molinaro J, Gregory F, Nash RJ, Arumugham J, Usta SN, Eilertson CD, Stice SL. 2011. GABRB3 gene expression increases upon ethanol exposure in human embryonic stem cells. J Recept Signal Transduct Res 31(3):206-213.
23. Miller MW. 1995. Generation of neurons in the rat dentate gyrus and hippocampus: effects of prenatal and postnatal treatment with ethanol. Alcohol Clin Exp Res 19(6):1500-1509.
24. Mooney SM, Miller MW. 2001. Effects of prenatal exposure to ethanol on the expression of bcl-2, bax and caspase 3 in the developing rat cerebral cortex and thalamus. Brain Res 911(1):71-81.
25. Muller YM, Kobus K, Schatz JC, Ammar D, Nazari EM. 2012. Prenatal lead acetate exposure induces apoptosis and changes GFAP expression during spinal cord development. Ecotoxicol Environ Saf 75(1):223-229.
26. Nash R, Krishnamoorthy M, Jenkins A, Csete M. 2012. Human embryonic stem cell model of ethanol-mediated early developmental toxicity. Exp Neurol 234(1):127-135.
27. Nash RJ, Heimburg-Molinaro J. 2009. Heparin binding epidermal growth factor-like growth factor reduces ethanol-induced apoptosis and differentiation in human embryonic stem cells. Growth Factors 27(6):362-369.
28. Nash RJ, Heimburg-Molinaro J, Nash RJ. 2009. Heparin binding epidermal growth factor-like growth factor reduces ethanol-induced apoptosis and differentiation in human embryonic stem cells. Growth Factors 27(6):362-369.
29. Nixon K, Crews FT. 2002. Binge ethanol exposure decreases neurogenesis in adult rat hippocampus. J Neurochem 83(5):1087-1093.
30. Pawlak R, Skrzypiec A, Sulkowski S, Buczko W. 2002. Ethanol-induced neurotoxicity is counterbalanced by increased cell proliferation in mouse dentate gyrus. Neurosci Lett 327(2):83-86.
31. Radin NS, Brenkert A, Arora RC, Sellinger OZ, Flangas AL. 1972. Glial and neuronal localization of cerebroside-metabolizing enzymes. Brain Res 39(1):163-169.
32. Rice AC, Bullock MR, Shelton KL. 2004. Chronic ethanol consumption transiently reduces adult neural progenitor cell proliferation. Brain Res 1011(1):94-98.
33. Roach J. 2005. 9, 000-year-old beer re-created from chinese recipe. National Geographic News 1-2.
34. Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD. 2007. Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue. Stem Cells 25(5):1298-1306.
35. Tabakoff B, Saba L, Printz M, Flodman P, Hodgkinson C, Goldman D, Koob G, Richardson HN, Kechris K, Bell RL, Hubner N, Heinig M, Pravenec M, Mangion J, Legault L, Dongier M, Conigrave KM, Whitfield JB, Saunders J, Grant B, Hoffman PL. 2009. Genetical genomic determinants of alcohol consumption in rats and humans. BMC Biol 7:70.
36. Tateno M, Ukai W, Ozawa H, Yamamoto M, Toki S, Ikeda H, Saito T. 2004. Ethanol inhibition of neural stem cell differentiation is reduced by neurotrophic factors. Alcohol Clin Exp Res 28(8):134s-138s.
37. Thomas S, Thomas M, Wincker P, Babarit C, Xu P, Speer MC, Munnich A, Lyonnet S, Vekemans M, Etchevers HC. 2008. Human neural crest cells display molecular and phenotypic hallmarks of stem cells. Hum Mol Genet 17(21):3411-3425.
38. Tropepe V, Sibilia M, Ciruna BG, Rossant J, Wagner EF, van der Kooy D. 1999. Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Dev Biol 208(1):166-188.
39. Valles S, Pitarch J, Renau-Piqueras J, Guerri C. 1997. Ethanol exposure affects glial fibrillary acidic protein gene expression and transcription during rat brain development. J Neurochem 69(6):2484-2493.