Assessment and quantification of telomerase enzyme activity

Methods in Molecular Biology

Volumn 965, Issue , 2013, Pages 215-231

  Maritz, Michelle F ^a,b   Richards, Laura A ^a,b   MacKenzie, Karen L ^a,b  

^a CHILDREN S CANCER INSTITUTE AUSTRALIA FOR MEDICAL RESEARCH   (Australia)

^b UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Q TRAP; Real time PCR; Telomerase; Telomeric repeat amplification protocol; TERT; TRAP

Indexed keywords

OLIGONUCLEOTIDE; REPETITIVE DNA; TELOMERASE; PRIMER DNA;

ADHERENT CELL; ARTICLE; BONE MARROW CELL; CELL LYSATE; ENZYME ACTIVITY; FEMALE; GENE AMPLIFICATION; HEAT TREATMENT; HELA CELL; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; LIMIT OF QUANTITATION; LUNG FIBROBLAST; NEUROBLASTOMA CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DETERMINATION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; TELOMERIC REPEAT AMPLIFICATION PROTOCOL; WHOLE CELL; CELL LINE; ENZYME ASSAY; GENETICS; METABOLISM; METHODOLOGY; NUCLEOTIDE REPEAT; TELOMERE;

CELL LINE; DNA PRIMERS; ENZYME ASSAYS; HUMANS; POLYMERASE CHAIN REACTION; REPETITIVE SEQUENCES, NUCLEIC ACID; TELOMERASE; TELOMERE;

MLCS; MLOWN;

EID: 84878890138     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-239-1_14     Document Type: Article

References (24)

1. Harley CB, Futcher AB, Greider CW (1990) Telomeres shorten during ageing of human fibroblasts. Nature 345:458-460
2. Hastie ND, Dempster M, Dunlop MG, Thompson AM, Green DK, Allshire RC (1990) Telomere reduction in human colorectal carcinoma and with ageing. Nature 346:866-868
3. Olovnikov AM (1973) A theory of marginotomy. The incomplete copying of template margin in enzymic synthesis of polynucleotides and biological significance of the phenomenon. J Theor Biol 41:181-190
4. Moyzis RK, Buckingham JM, Cram LS, Dani M, Deaven LL, Jones MD, Meyne J, Ratliff RL, Wu JR (1988) A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci U S A 85:6622-6626
5. McClintock B (1941) The stability of broken ends of chromosomes in Zea mays. Genetics 26:234-282
6. Blackburn EH, Gall JG (1978) A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol 120:33-53
7. d'Adda di Fagagna F, Reaper PM, Clay-Farrace L, Fiegler H, Carr P, Von Zglinicki T, Saretzki G, Carter NP, Jackson SP (2003) A DNA damage checkpoint response in telomere-initiated senescence. Nature 426:194-198
8. Blackburn EH, Greider CW, Henderson E, Lee MS, Shampay J, Shippen-Lentz D (1989) Recognition and elongation of telomeres by telomerase. Genome 31:553-560
9. Greider CW, Blackburn EH (1989) A telomeric sequence in the RNA of tetrahymena telomerase required for telomere repeat synthesis. Nature 337:331-337
10. Morin GB (1989) The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 59:521-529
11. Mitchell JR, Wood E, Collins K (1999) A telomerase component is defective in the human disease dyskeratosis congenita. Nature 402:551-555
12. Cohen SB, Graham ME, Lovrecz GO, Bache N, Robinson PJ, Reddel RR (2007) Protein composition of catalytically active human telomerase from immortal cells. Science 315:1850-1853
13. Greider CW, Blackburn EH (1985) Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43:405-413
14. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich SL, Shay JW (1994) Specific association of human telomerase activity with immortal cells and cancer. Science 266:2011-2015
15. Kim NW, Wu F (1997) Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP). Nucleic Acids Res 25:2595-2597
16. Counter CM, Gupta J, Harley CB, Leber B, Bacchetti S (1995) Telomerase activity in normal leukocytes and in hematologic malignancies. Blood 85:2315-2320
17. Broccoli D, Young JW, de Lange T (1995) Telomerase activity in normal and malignant hematopoietic cells. Proc Natl Acad Sci U S A 92:9082-9086
18. Hiyama K, Hirai Y, Kyoizumi S, Akiyama M, Hiyama E, Piatyszek MA, Shay JW, Ishioka S, Yamakido M (1995) Activation of telomerase in human lymphocytes and hematopoietic progenitor cells. J Immunol 155:3711-3715
19. Wright WE, Piatyszek MA, Rainey WE, Byrd W, Shay JW (1996) Telomerase activity in human germline and embryonic tissues and cells. Dev Genet 18:173-179
20. Falchetti ML, Levi A, Molinari P, Verna R, D'Ambrosio E (1998) Increased sensitivity and reproducibility of TRAP assay by avoiding direct primers interaction. Nucleic Acids Res 26:862-863
21. Herbert BS, Hochreiter AE, Wright WE, Shay JW (2006) Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol. Nat Protoc 1:1583-1590
22. Hou M, Xu D, Bjorkholm M, Gruber A (2001) Real-time quantitative telomeric repeat amplification protocol assay for the detection of telomerase activity. Clin Chem 47:519-524
23. Fu B, Quintero J, Baker CC (2003) Keratinocyte growth conditions modulate telomerase expression, senescence, and immortalization by human papillomavirus type 16 E6 and E7 oncogenes. Cancer Res 63: 7815-7824
24. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402-408