Probing Ca2+ -binding capability of viral proteins with the ef-hand motif by grafting approach

Methods in Molecular Biology

Volumn 963, Issue , 2013, Pages 37-53

  Zhou, Yubin ^a   Xue, Shenghui ^a   Chen, Yanyi ^a   Yang, Jenny J ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

Author keywords

Calcium; CD2; EF hand; Luminescence resonance energy transfer; Protein engineering; Protein ligand interaction; Terbium; Virus

Indexed keywords

CALCIUM BINDING PROTEIN; CALCIUM ION; NONSTRUCTURAL PROTEIN 1; VIRAL PROTEIN; CALCIUM; VIRUS PROTEIN;

AMINO ACID SEQUENCE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CALCIUM BINDING; CIRCULAR DICHROISM; NONHUMAN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; SINDBIS VIRUS; VIRUS ENTRY; VIRUS GENOME; ARTICLE; AUTOMATED PATTERN RECOGNITION; BINDING SITE; BIOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; LUMINESCENCE; METABOLISM; METHODOLOGY; PROTEIN BINDING; PROTEIN MOTIF; PROTEIN TERTIARY STRUCTURE; TOGAVIRUS;

BINDING SITES; CALCIUM; COMPUTATIONAL BIOLOGY; EF HAND MOTIFS; GENOME, VIRAL; LUMINESCENT MEASUREMENTS; MODELS, MOLECULAR; PATTERN RECOGNITION, AUTOMATED; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; TOGAVIRIDAE; VIRAL PROTEINS;

EID: 84878748730     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-230-8_3     Document Type: Chapter

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