Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining

Methods in Molecular Biology

Volumn 967, Issue , 2013, Pages 179-188

  Khorooshi, Reza ^a   Owens, Trevor ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

Astrocytes; Central nervous system; Entorhinal cortex lesion; Immunohistochemistry; Phospho STAT2

Indexed keywords

STAT2 PROTEIN; PHOSPHOPROTEIN;

ADULT; ANIMAL EXPERIMENT; BRAIN TISSUE; CELLULAR DISTRIBUTION; CENTRAL NERVOUS SYSTEM; COMPARATIVE STUDY; CONTROLLED STUDY; DENTATE GYRUS; ENTORHINAL CORTEX; FEMALE; HIPPOCAMPUS; IMMUNOHISTOCHEMISTRY; LIGHT EXPOSURE; MOUSE; NONHUMAN; PERFORANT NERVE TRACT; PROTEIN PHOSPHORYLATION; ANIMAL; ARTICLE; METABOLISM; METHODOLOGY; NERVE FIBER; PATHOLOGY; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; STAINING;

ANIMALS; AXONS; ENTORHINAL CORTEX; FEMALE; HIPPOCAMPUS; IMMUNOHISTOCHEMISTRY; MICE; PHOSPHOPROTEINS; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; STAINING AND LABELING; STAT2 TRANSCRIPTION FACTOR;

EID: 84878746785     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-242-1_13     Document Type: Chapter

References (17)

1. Theofilopoulos AN, Baccala R, Beutler B, Kono DH (2005) Type I interferons (alpha/beta) in immunity and autoimmunity. Annu Rev Immunol 23:307–336.
2. Testoni B, Vollenkle C, Guerrieri F, Gerbal-Chaloin S, Blandino G, Levrero M (2011) Chromatin dynamics of gene activation and repression in response to interferon alpha (IFN(alpha)) reveal new roles for phosphorylated and unphosphorylated forms of the transcription factor STAT2. J Biol Chem 286:20217–20227.
3. Stark GR, Kerr IM, Williams BR, Silverman RH, Schreiber RD (1998) How cells respond to interferons. Annu Rev Biochem 67: 227–264.
4. Schindler C, Levy DE, Decker T (2007) JAK-STAT signaling: from interferons to cytokines. J Biol Chem 282:20059–20063.
5. Levy DE, Darnell JE Jr (2002) Stats: transcriptional control and biological impact. Nat Rev Mol Cell Biol 3:651–662.
6. Burks J (2005) Interferon-beta1b for multiple sclerosis. Expert Rev Neurother 5:153–164.
7. Teige I, Treschow A, Teige A, Mattsson R, Navikas V, Leanderson T, Holmdahl R, Issazadeh-Navikas S (2003) IFN-beta gene deletion leads to augmented and chronic demyelinating experimental autoimmune encephalomyelitis. J Immunol 170:4776–4784.
8. Prinz M, Schmidt H, Mildner A, Knobeloch KP, Hanisch UK, Raasch J, Merkler D, Detje C, Gutcher I, Mages J, Lang R, Martin R, Gold R, Becher B, Bruck W, Kalinke U (2008) Distinct and nonredundant in vivo functions of IFNAR on myeloid cells limit autoimmunity in the central nervous system. Immunity 28:675–686.
9. Guo B, Chang EY, Cheng G (2008) The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. J Clin Invest 118:1680–1690.
10. Khorooshi R, Owens T (2010) Injury-Induced Type I IFN Signaling Regulates Inflammatory Responses in the Central Nervous System. J Immunol 185(2):1258–1264.
11. Finsen B, Jensen MB, Lomholt ND, Hegelund IV, Poulsen FR, Owens T (1999) Axotomy-induced glial reactions in normal and cytokine transgenic mice. Adv Exp Med Biol 468:157–171.
12. Ladeby R, Wirenfeldt M, Dalmau I, Gregersen R, Garcia-Ovejero D, Babcock A, Owens T, Finsen B (2005) Proliferating resident microglia express the stem cell antigen CD34 in response to acute neural injury. Glia 50: 121–131.
13. Babcock AA, Wirenfeldt M, Holm T, Nielsen HH, Dissing-Olesen L, Toft-Hansen H, Millward JM, Landmann R, Rivest S, Finsen B, Owens T (2006) Toll-like receptor 2 signaling in response to brain injury: an innate bridge to neuroinflammation. J Neurosci 26: 12826–12837.
14. Wirenfeldt M, Dissing-Olesen L, Anne Babcock A, Nielsen M, Meldgaard M, Zimmer J, Azcoitia I, Leslie RG, Dagnaes-Hansen F, Finsen B (2007) Population control of resident and immigrant microglia by mitosis and apoptosis. Am J Pathol 171:617–631.
15. Khorooshi R, Babcock AA, Owens T (2008) NF-kappaB-driven STAT2 and CCL2 expression in astrocytes in response to brain injury. J Immunol 181:7284–7291.
16. Munzberg H, Huo L, Nillni EA, Hollenberg AN, Bjorbaek C (2003) Role of signal transducer and activator of transcription 3 in regulation of hypothalamic proopiomelanocortin gene expression by leptin. Endocrinology 144:2121–2131.
17. Park C, Li S, Cha E, Schindler C (2000) Immune response in Stat2 knockout mice. Immunity 13:795–804.