Designed construction of recombinant DNA at the ura3Δ0 locus in the yeast Saccharomyces cerevisiae

Yeast

Volumn 30, Issue 6, 2013, Pages 243-253

  Fukunaga, Tomoaki ^a   Cha aim, Kamonchai ^a,c   Hirakawa, Yuki ^a   Sakai, Ryota ^a   Kitagawa, Takao ^a   Nakamura, Mikiko ^b   Nonklang, Sanom ^a,d   Hoshida, Hisashi ^a   Akada, Rinji ^a  

^a YAMAGUCHI UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b YAMAGUCHI UNIVERSITY   (Japan)

^c SRINAKHARINWIROT UNIVERSITY   (Thailand)

^d UBON RATCHATHANI UNIVERSITY   (Thailand)

Author keywords

Homologous recombination; Overlap extension PCR; Recombinant DNA; ura3

Indexed keywords

RECOMBINANT DNA;

ARTICLE; CODON; GENE EXPRESSION; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

DNA, RECOMBINANT; GENE EXPRESSION REGULATION, FUNGAL; GENE TARGETING; GENETIC LOCI; HOMOLOGOUS RECOMBINATION; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 84878704848     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.2957     Document Type: Article

References (27)

1. Abdel-Banat BM, Nonklang S, Hoshida H, Akada R. 2010. Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianus. Yeast 27: 29-39.
2. Akada R, Hirosawa I, Kawahata M, et al. 2002. Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeast. Yeast 19: 393-402.
3. Akada R, Kitagawa T, Kaneko S, et al. 2006. PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae. Yeast 23: 399-405.
4. Akada R, Murakane T, Nishizawa Y. 2000. DNA extraction method for screening yeast clones by PCR. Biotechniques 28: 668-674.
5. Alberti S, Gitler AD, Lindquist S. 2007. A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae. Yeast 24: 913-919.
6. Ano A, Suehiro D, Cha-Aim K, et al. 2009. Combinatorial gene overexpression and recessive mutant gene introduction in sake yeast. Biosci Biotechnol Biochem 73: 633-640.
7. Ausubel FM, Brent R, Kingston RE, et al. (eds). 1999. Short Protocols in Molecular Biology. Wiley: New York.
8. Brachmann CB, Davies A, Cost GJ, et al. 1998. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132.
9. Cha-aim K, Fukunaga T, Hoshida H, Akada R. 2009. Reliable fusion PCR mediated by GC-rich overlap sequences. Gene 434: 43-49.
10. Cha-aim K, Hoshida H, Fukunaga T, Akada R. 2012. Fusion PCR via novel overlap sequences. In Gene Synthesis Methods and Protocols, Peccoud J (ed). Humana: New York; 97-110.
11. Chino A, Watanabe K, Moriya H. 2010. Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe. PLoS One 5: e9652.
12. Ghaemmaghami S, Huh WK, Bower K, et al. 2003. Global analysis of protein expression in yeast. Nature 425: 737-741.
13. Hashimoto S, Ogura M, Aritomi K, et al. 2005. Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis. Appl Environ Microbiol 71: 312-319.
14. Johnston M, Davis RW. 1984. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol 4: 1440-1448.
15. Kaiser C, Michaelis S, Mitchell A. 1994. Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY.
16. Kouprina N, Campbell M, Graves J, et al. 1998. Construction of human chromosome 16- and 5-specific circular YAC/BAC libraries by in vivo recombination in yeast (TAR cloning). Genomics 53: 21-28.
17. Kuroda S, Otaka S, Fujisawa Y. 1994. Fermentable and nonfermentable carbon sources sustain constitutive levels of expression of yeast triosephosphate dehydrogenase 3 gene from distinct promoter elements. J Biol Chem 269: 6153-6162.
18. Ma H, Kunes S, Schatz PJ, Botstein D. 1987. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216.
19. Mascorro-Gallardo JO, Covarrubias AA, Gaxiola R. 1996. Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae. Gene 172: 169-170.
20. Matsuyama A, Yoshida M. 2009. Systematic cloning of an ORFeome using the Gateway system. Methods Mol Biol 577: 11-24.
21. McGinnis MR, Padhye AA, Ajello L. 1974. Storage of stock cultures of filamentous fungi, yeasts, and some aerobic actinomycetes in sterile distilled water. Appl Microbiol 28: 218-222.
22. Mirisola MG, Colomba L, Amodeo R, De Leo G. 2007. Yeast vectors for the integration/expression of any sequence at the TYR1 locus. Yeast 24: 761-766.
23. Oldenburg KR, Vo KT, Michaelis S, Paddon C. 1997. Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucleic Acids Res 25: 451-452.
24. Petersen JG, Holmberg S. 1986. The ILV5 gene of Saccharomyces cerevisiae is highly expressed. Nucleic Acids Res 14: 9631-9651.
25. Storck T, Krüth U, Kolhekar R, et al. 1996. Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse. Nucleic Acids Res 24: 4594-4596.
26. Suzuki Y, Kagawa N, Fujino T, et al. 2005. A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system. Nucleic Acids Res 33: e109.
27. Tochigi Y, Sato N, Sahara T, et al. 2010. Sensitive and convenient yeast reporter assay for high-throughput analysis by using a secretory luciferase from Cypridina noctiluca. Anal Chem 82: 5768-5776.