A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system

Nucleic Acids Research

Volumn 33, Issue 12, 2005, Pages 1-6

  Suzuki, Yasuhiro ^a   Kagawa, Naoko ^a,b   Fujino, Toru ^a,b   Sumiya, Tsuyoshi ^b   Andoh, Taichi ^a,b   Ishikawa, Kumiko ^a,b   Kimura, Rie ^b   Kemmochi, Kiyokazu ^a   Ohta, Tsutomu ^c   Tanaka, Shigeo ^a,b  

^a Yokohama Kanazawa High Tech Center   (Japan)

^b Koto ku   (Japan)

^c NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PLASMID VECTOR; CIRCULAR DNA; HYBRID PROTEIN;

AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHIMERA; CONTROLLED STUDY; DNA TEMPLATE; DRUG DESIGN; ESCHERICHIA COLI; GENE TECHNOLOGY; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; HIGH THROUGHPUT SCREENING; HOMOLOGOUS RECOMBINATION; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURAL BIOINFORMATICS; BACTERIUM TRANSFORMATION; CHEMISTRY; DNA SHUFFLING; EVALUATION; GENE VECTOR; GENETICS; METHODOLOGY; PLASMID;

CLONING, MOLECULAR; DNA SHUFFLING; DNA, CIRCULAR; ESCHERICHIA COLI; GENETIC VECTORS; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSFORMATION, BACTERIAL;

EID: 26444593986     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gni103     Document Type: Article

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