N-glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable α-amylase in Saccharomyces cerevisiae

Applied Microbiology and Biotechnology

Volumn 97, Issue 12, 2013, Pages 5473-5482

  Hoshida, Hisashi ^a   Fujita, Tsuneyasu ^a   Cha Aim, Kamonchai ^a,b   Akada, Rinji ^a  

^a YAMAGUCHI UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b SRINAKHARINWIROT UNIVERSITY   (Thailand)

Author keywords

Genome wide screening; Heterologous production; N glycosylation; Saccharomyces cerevisiae; Thermostable amylase

Indexed keywords

BACILLUS LICHENIFORMIS; ENGINEERING APPROACHES; HETEROLOGOUS PRODUCTION; N-GLYCOSYLATION; N-GLYCOSYLATION SITES; N-LINKED GLYCOSYLATION; OLIGOSACCHARIDE SYNTHESIS; OLIGOSACCHARYL TRANSFERASE;

AMYLASES; BACTERIOLOGY; ESTERIFICATION; GENE ENCODING; GENETIC ENGINEERING; GLYCOSYLATION; OLIGOSACCHARIDES;

YEAST;

AMYLASE; ASPARAGINE LINKED OLIGOSACCHARIDE;

BACTERIUM; ENZYME; ENZYME ACTIVITY; GENETIC ENGINEERING; MUTATION; SECRETION; YEAST;

ARTICLE; BACILLUS LICHENIFORMIS; CARBOHYDRATE SYNTHESIS; CONTROLLED STUDY; ENZYME STABILITY; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; IMMUNOBLOTTING; KNOCKOUT GENE; NONHUMAN; SACCHAROMYCES CEREVISIAE; WILD TYPE; YEAST;

ALPHA-AMYLASES; BACILLUS; DNA MUTATIONAL ANALYSIS; GENE KNOCKOUT TECHNIQUES; GLYCOSYLATION; METABOLIC ENGINEERING; MUTATION, MISSENSE; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

BACILLUS LICHENIFORMIS; SACCHAROMYCES CEREVISIAE;

EID: 84878680062     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4582-2     Document Type: Article

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