Application of cell-specific isolation to the study of dopamine signaling in Drosophila

Methods in Molecular Biology

Volumn 964, Issue , 2013, Pages 215-225

  Iyer, Eswar Prasad R ^a   Iyer, Srividya Chandramouli ^a   Cox, Daniel N ^b  

^a GEORGE MASON UNIVERSITY   (United States)

^b GEORGE MASON UNIVERSITY   (United States)

Author keywords

Brain; Dopamine signaling; Drosophila; GAL4 UAS; Magnetic bead cell sorting; RNA Isolation

Indexed keywords

DOPAMINE; RNA; ANTIBODY; MICROSPHERE;

ADULT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ISOLATION; CELL SELECTION; CONTROLLED STUDY; DOPAMINERGIC NERVE CELL; DROSOPHILA; GENE EXPRESSION PROFILING; MAGNET; MAGNETIC BEAD CELL SORTING; MICROARRAY ANALYSIS; NERVOUS SYSTEM; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SIGNAL TRANSDUCTION; ANIMAL; CELL SEPARATION; CYTOLOGY; DROSOPHILA MELANOGASTER; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; SUSPENSION;

ANIMALS; ANTIBODIES; CELL SEPARATION; DOPAMINE; DROSOPHILA MELANOGASTER; MAGNETS; MICROSPHERES; RNA; SIGNAL TRANSDUCTION; SUSPENSIONS;

MLCS; MLOWN;

EID: 84878662395     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-251-3_13     Document Type: Article

References (15)

1. Kim YC, Lee HG, Han KA (2007) D1 dopamine receptor dDA1 is required in the mushroom body neurons for aversive and appetitive learning in Drosophila. J Neurosci 27:7640-7647
2. Selcho M, Pauls D, Han K-A, Stocker RF, Thum AS (2009) The role of dopamine in Drosophila larval classical olfactory conditioning. PLoS One 4:e5897. doi: 10.1371/journal. pone.0005897
3. Ward A, Walker VJ, Feng Z, Xu XZS (2009) Cocaine modulates locomotion behavior in C. elegans . PLoS One 4:e5946. doi: 10.1371/ journal.pone.0005946
4. Lebestky T, Chang JS, Dankert H, Zelnik L, Kim YC, Han KA, Wolf FW, Perona P, Anderson DJ (2009) Two different forms of arousal in Drosophila are oppositely regulated by the dopamine D1 receptor ortholog DopR via distinct neural circuits. Neuron 64:522-536
5. Bainton RJ, Tsai LT-Y, Singh CM, Moore MS, Neckameyer WS, Heberlein U (2000) Dopamine modulates acute responses to cocaine, nicotine and ethanol in Drosophila . Curr Biol 10:187-194
6. Blenau W, Baumann A (2001) Molecular and pharmacological properties of insect biogenic amine receptors: lessons from Drosophila melanogaster and Apis mellifera . Arch Insect Biochem Physiol 48:13-38
7. Wolf FW, Heberlein U (2003) Invertebrate models of drug abuse. J Neurobiol 54:161-178
8. McClung C, Hirsh J (1998) Stereotypic behavioral responses to free-base cocaine and the development of behavioral sensitization in Drosophila . Curr Biol 8:109-112
9. Rothenfluh A, Heberlein U (2002) Drugs, flies, and videotape: the effects of ethanol and cocaine on Drosophila locomotion. Curr Opin Neurobiol 12:639-645
10. Heberlein U, Wolf FW, Rothenfluh A, Guarnieri DJ (2004) Molecular genetic analysis of ethanol intoxication in Drosophila melanogaster . Integr Comp Biol 44:269-274
11. Heberlein U, Tsai LT, Kapfhamer D, Lasek AW (2009) Drosophila, a genetic model system to study cocaine-related behaviors: a review with focus on LIM-only proteins. Neuropharmacology 56:97-106
12. Iyer EPR, Iyer SC, Sulkowski MJ, Cox DN (2009) Isolation and purification of Drosophila peripheral neurons by magnetic bead sorting. J Vis Exp 34:e1599. doi: 10.3791/1599 (2009).
13. Wang X, Bo J, Bridges T, Dugan KD, Pan T-C, Chodosh L, Montell DJ (2006) Analysis of cell migration using whole genome expression profiling of migratory cells in the Drosophila ovary. Dev Cell 10:483-495
14. Friggi-Grelin F, Coulom H, Meller M, Gomez D, Hirsh J, Birman S (2003) Targeted gene expression in Drosophila dopaminergic cells using regulatory sequences from tyrosine hydroxylase. J Neurobiol 54:618-627
15. Wu JS, Luo L (2006) A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining. Nat Protoc 1:2110-2115