Isolation and purification of Drosophila peripheral neurons by magnetic bead sorting

Journal of Visualized Experiments

Volumn , Issue 34, 2009, Pages

  Iyer, Eswar Prasad R ^a   Iyer, Srividya Chandramouli ^a   Sulkowski, Mikolaj J ^a   Cox, Daniel N ^a  

^a GEORGE MASON UNIVERSITY   (United States)

Author keywords

Cell dissociation; Cell isolation; Dendritic arborization neurons; Drosophila; Magnetic bead sorting; Microarray; Neuron; Peripheral Nervous System (PNS); RNA

Indexed keywords

ANIMAL; ARTICLE; CYTOLOGY; DENDRITE; DROSOPHILA; MAGNETISM; METHODOLOGY; MOUSE; NERVE CELL; PERIPHERAL NERVOUS SYSTEM; PHYSIOLOGY;

ANIMALS; DENDRITES; DROSOPHILA; MAGNETICS; MICE; NEURONS; PERIPHERAL NERVOUS SYSTEM;

EID: 79960620969     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1599     Document Type: Article

References (6)

1. Corty, M.M., Matthews, B.J. and Grueber, W.B. Molecules and mechanisms of dendrite development in Drosophila. Development 136, 1049-1061 (2009).
2. Parrish, J.Z., Emoto, K., Kim, M.D., and Jan, Y.N. Mechanisms that regulate establishment, maintenance, and remodeling of dendritic fields. Ann. Rev. Neurosci. 30, 399-423 (2007).
3. Lee, T. and Luo, L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22, 451-461 (1999).
4. Grueber, W.B. et al. Projections of Drosophila multidendritic neurons in the central nervous system: links with peripheral dendrite morphology. Development 134, 55-64 (2007).
5. Song, W., Onishi, M., Jan, L.Y. and Jan, Y.N. Peripheral multidendritic sensory neurons are necessary for rhythmic locomotion behavior in Drosophila larvae. Proc Natl Acad Sci USA 104, 5199-5204 (2007).
6. Livak, K.J. and Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T) methods. Methods 25, 402-408 (2001).