Specific detection of Pneumocystis jirovecii in clinical samples by flow cytometry

Methods in Molecular Biology

Volumn 968, Issue , 2013, Pages 203-211

  Barbosa, Joana ^a,b   Costa De Oliveira, Sofia ^a   Silva, Ana Teresa ^a   Rodrigues, Acácio Gonçalves ^a   Pina Vaz, Cidália ^a  

^a University of Porto   (Portugal)

^b INSTITUTO PIAGET   (Portugal)

Author keywords

Emergent fungi; Flow cytometry; Immunocompromised patients; Pneumocystis jirovecii diagnosis; Pneumonia; Respiratory samples; Specific monoclonal antibodies

Indexed keywords

MONOCLONAL ANTIBODY;

ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FLOW CYTOMETRY; FUNGAL DETECTION; HUMAN; IMMUNOFLUORESCENCE; LUNG LAVAGE; NONHUMAN; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY; STAINING;

BRONCHOALVEOLAR LAVAGE FLUID; FLOW CYTOMETRY; HUMANS; PNEUMOCYSTIS JIROVECII; STAINING AND LABELING;

MLCS; MLOWN;

EID: 84878653743     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-257-5_16     Document Type: Article

References (28)

1. Murray PR, Rosenthal KS, Pfaller MA (2005) Fungal classi fication, structure, and replication. In: Murray PR, Rosenthal KS, Pfaller MA (eds) Medical microbiology, 5th edn. Mosby, Philadelphia, Pennsylvania, pp 67-73
2. Su YS, Lu JJ, Perng CL, Chang FY (2008) Pneumocystis jirovecii pneumonia in patients with and without human immunode ficiency virus infection. J Microbiol Immunol Infect 41:478-4823
3. Kelley CF, Checkley W, Mannino DM, Franco-Paredes C, Del Rio C, Holguin F (2009) Trends in hospitalizations for AIDS associated Pneumocystis jirovecii pneumonia in the United States (1986 to 2005). Chest 136:190-197
4. Procop GW, Haddad S, Quinn J, Wilson ML, Henshaw NG, Reller LB, Artymyshyn RL, Katanik MT, Weinstein MP (2004) Detection of Pneumocystis jirovecii in respiratory specimens by four staining methods. J Clin Microbiol 42:3333-3335
5. Nevez G, Chabé M, Rabodonirina M, Virmaux M, Dei-Cas E, Hauser PM, Totet A (2008) Nosocomial Pneumocystis jirovecii infections. Parasite 15:359-365
6. Morris A, Netravali M, Kling HM, Shipley T, Ross T, Sciurba FC, Norris KA (2008) Relationship of Pneumocystis antibody response to severity of chronic obstructive pulmonary disease. Clin Infect Dis 47:e64-e68
7. Krajicek BJ, Thomas CF Jr, Limper AH (2009) Pneumocystis pneumonia: current concepts in pathogenesis, diagnosis, and treatment. Clin Chest Med 30:265-278
8. Durand-Joly I, Chabé M, Soula F, Delhaes L, Camus D, Dei-Cas E (2005) Molecular diagnosis of Pneumocystis pneumonia. FEMS Immunol Med Microbiol 45:405-410
9. Jiancheng W, Minjun H, Yi-jun A, Lan S, Zengzhu G, Jianrong S, Xixiong K (2009) Screening Pneumocystis carinii pneumonia in non-HIV-infected immunocompromised patients using polymerase chain reaction. Diagn Microbiol Infect Dis 64:396-401
10. Alvarez-Martínez MJ, Miró JM, Valls ME, Moreno A, Rivas PV, Solé M, Benito N, Domingo P, Muñoz C, Rivera E, Zar HJ, Wissmann G, Diehl AR, Prolla JC, de Anta MT, Gatell JM, Wilson PE, Meshnick SR (2006) Sensitivity and speci ficity of nested and real-time PCR for the detection of Pneumocystis jirovecii in clinical specimens. Diagn Microbiol Infect Dis 56:153-160
11. Robberts FJL, Liebowitz LD, Chalkley LJ (2007) Polymerase chain reaction detection of Pneumocystis jirovecii : evaluation of 9 assays. Diagn Microbiol Infect Dis 58:385-392
12. Watson JV (1991) Introduction to flow cytometry, 1st edn. Cambridge University Press, Cambridge
13. Álvarez-Barrientos A, Arroyo J, Cantón R, Nombela C, Sánchez-Pérez M (2000) Applications of flow cytometry to clinical microbiology. Clin Microbiol Rev 13:167-195
14. Givan A (2005) Flow cytometry: an introduction. In: Hawley TS, Hawley RG (eds) Methods in molecular biology: flow cytometry protocols, 2nd edn. Humana Press, Totowa, NJ, pp 1-32
15. Shapiro HM, Nebe-von G (2005) Multiparameter flow cytometry of bacteria. In: Hawley TS, Hawley RG (eds) Methods in molecular biology: flow cytometry protocols, 2nd edn. Humana Press, Totowa, NJ, pp 33-43
16. Montemayor M, Galofré B, Ribas F, Lucena F (2007) Comparative study between two laser scanning cytometers and epi fl uorescence microscopy for detection of Cryptosporidium oocysts in water. Cytometry A 71:173-179
17. Pina-Vaz C, Rodrigues AG (2010) Evaluation of antifungal susceptibility using flow cytometry. Methods Mol Biol 638:281-289
18. Pina-Vaz C, Rodrigues AG, Sansonetty F, Martinez-De-Oliveira J, Fonseca AF, Mårdh P-A (2000) Antifungal activity of local anesthetics against Candida species. Infect Dis Obstet Gynecol 8:124-137
19. Pina-Vaz C, Sansonetty F, Rodrigues AG, Martinez-De-Oliveira J, Fonseca AF, Mårdh P-A (2000) Antifungal activity of ibuprofen alone and in combination with fl uconazole against Candida species. J Med Microbiol 49:831-840
20. Pina-Vaz C, Sansonetty F, Rodrigues AG, Costa-Oliveira S, Tavares C, Martinezde-Oliveira J (2001) Cytometric approach for a rapid evaluation of susceptibility of Candida strains to antifungals. Clin Microbiol Infect 7:609-618
21. Pina-Vaz C, Costa-de-Oliveira S, Rodrigues AG, Espinel-Ingroff A (2005) Comparison of two probes for testing susceptibilities of pathogenic yeasts to voriconazole, itraconazole, and caspofungin by flow cytometry. J Clin Microbiol 43:4674-4679
22. Pina-Vaz C, Rodrigues AG, Costa-de-Oliveira S, Ricardo E, Mårdh P-A (2005) Potent synergic effect between ibuprofen and azoles on Candida resulting from blockade of ef fl ux pumps as determined by FUN-1 staining and flow cytometry. J Antimicrob Chemother 56:678-685
23. Barbosa J, Bragada C, Costa-de-Oliveira S, Ricardo E, Rodrigues AG, Pina-Vaz C (2010) A new method for the detection of Pneumocystis jirovecii using flow cytometry. Eur J Clin Microbiol Infect Dis 29:1147-1152
24. Pina-Vaz C, Costa-Oliveira S, Rodrigues AG, Salvador A (2004) Novel method using a laser scanning cytometer for detection of mycobacteria in clinical samples. J Clin Microbiol 42:906-908
25. Pina-Vaz C, Costa-de-Oliveira S, Rodrigues AG (2005) Safe susceptibility testing of Mycobacterium tuberculosis by flow cytometry with the fl uorescent nucleic acid stain SYTO 16. J Med Microbiol 54:77-81
26. Barbosa JM, Costa-de-Oliveira S, Rodrigues AG, Hanscheid T, Shapiro H, Pina-Vaz C (2008) A flow cytometric protocol for detection of Cryptosporidium spp. Cytometry A 73:44-47
27. Barbosa J, Costa-de-Oliveira S, Rodrigues AG, Pina-Vaz C (2008) Optimization of a flow cytometry protocol for detection and viability assessment of Giardia lamblia . Travel Med Infect Dis 6:234-239
28. Barbosa J, Rodrigues AG, Pina-Vaz C (2009) Cytometric approach for detection of Encephalitozoon intestinalis, an emergent agent. Clin Vaccine Immunol 16:1021-1024