A new method for the detection of Pneumocystis jirovecii using flow cytometry

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 29, Issue 9, 2010, Pages 1147-1152

  Barbosa, J ^a,b   Bragada, C ^a   Costa De Oliveira, S ^a   Ricardo, E ^a   Rodrigues, A G ^a,c   Pina Vaz, C ^a,c  

^a University of Porto   (Portugal)

^b INSTITUTO PIAGET   (Portugal)

^c HOSPITAL DE SÃO JOÃO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BODY FLUID; EVALUATION; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MYCOLOGY; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; SENSITIVITY AND SPECIFICITY;

BODY FLUIDS; FLOW CYTOMETRY; HUMANS; MICROSCOPY, FLUORESCENCE; MYCOLOGY; PNEUMOCYSTIS JIROVECII; PNEUMONIA, PNEUMOCYSTIS; SENSITIVITY AND SPECIFICITY;

EID: 78349231679     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-010-0980-5     Document Type: Article

References (22)

1. Murray PR, Rosenthal KS, Pfaller MA (2005) Fungal classification, structure, and replication. In: Murray PR, Rosenthal KS, Pfaller MA (eds) Medical microbiology, 5th edn. Mosby, Philadelphia, Pennsylvania, pp 67-73
2. Stringer JR, Beard CB, Miller RF, Wakefield AE (2002) A new name (Pneumocystis jiroveci) for Pneumocystis from humans. Emerg Infect Dis 8:891-896
3. Kelley CF, Checkley W, Mannino DM, Franco-Paredes C, Del Rio C, Holguin F (2009) Trends in hospitalizations for AIDS-associated Pneumocystis jirovecii pneumonia in the United States (1986 to 2005). Chest 136:190-197
4. Su YS, Lu JJ, Perng CL, Chang FY (2008) Pneumocystis jirovecii pneumonia in patients with and without human immunodeficiency virus infection. J Microbiol Immunol Infect 41:478-482
5. Wazir JF, Ansari NA (2004) Pneumocystis carinii infection. Update and review. Arch Pathol Lab Med 128:1023-1027
6. Krajicek BJ, Thomas CF Jr, Limper AH (2009) Pneumocystis pneumonia: current concepts in pathogenesis, diagnosis, and treatment. Clin Chest Med 30:265-278
7. Morris A, Sciurba FC, Norris KA (2008) Pneumocystis: a novel pathogen in chronic obstructive pulmonary disease? COPD 5:43-51
8. Maschmeyer G, Beinert T, Buchheidt D, Cornely OA, Einsele H, Heinz W, Heussel CP, Kahl C, Kiehl M, Lorenz J, Hof H, Mattiuzzi G (2009) Diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients: guidelines of the infectious diseases working party of the German Society of Haematology and Oncology. Eur J Cancer 45:2462-2472
9. Morris A, Netravali M, Kling HM, Shipley T, Ross T, Sciurba FC, Norris KA (2008) Relationship of pneumocystis antibody response to severity of chronic obstructive pulmonary disease. Clin Infect Dis 47:e64-e68
10. Nevez G, Chabé M, Rabodonirina M, Virmaux M, Dei-Cas E, Hauser PM, Totet A (2008) Nosocomial Pneumocystis jirovecii infections. Parasite 15:359-365
11. Procop GW, Haddad S, Quinn J, Wilson ML, Henshaw NG, Reller LB, Artymyshyn RL, Katanik MT, Weinstein MP (2004) Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. J Clin Microbiol 42:3333-3335
12. Durand-Joly I, Chabé M, Soula F, Delhaes L, Camus D, Dei-Cas E (2005) Molecular diagnosis of Pneumocystis pneumonia. FEMS Immunol Med Microbiol 45:405-410
13. Jiancheng W, Minjun H, Yi-jun A, Lan S, Zengzhu G, Jianrong S, Xixiong K (2009) Screening Pneumocystis carinii pneumonia in non-HIV-infected immunocompromised patients using polymerase chain reaction. Diagn Microbiol Infect Dis 64:396-401
14. Alvarez-Martínez MJ, Miró JM, Valls ME, Moreno A, Rivas PV, Solé M, Benito N, Domingo P, Muñoz C, Rivera E, Zar HJ, Wissmann G, Diehl AR, Prolla JC, de Anta MT, Gatell JM, Wilson PE, Meshnick SR (2006) Sensitivity and specificity of nested and real-time PCR for the detection of Pneumocystis jiroveci in clinical specimens. Diagn Microbiol Infect Dis 56:153-160
15. Robberts FJL, Liebowitz LD, Chalkley LJ (2007) Polymerase chain reaction detection of Pneumocystis jiroveci: evaluation of 9 assays. Diagn Microbiol Infect Dis 58:385-392
16. Barbosa JM, Costa-de-Oliveira S, Rodrigues AG, Hanscheid T, Shapiro H, Pina-Vaz C (2008) A flow cytometric protocol for detection of Cryptosporidium spp. Cytometry A 73:44-47
17. Barbosa J, Costa-de-Oliveira S, Rodrigues AG, Pina-Vaz C (2008) Optimization of a flow cytometry protocol for detection and viability assessment of Giardia lamblia. Travel Med Infect Dis 6:234-239
18. Barbosa J, Rodrigues AG, Pina-Vaz C (2009) Cytometric approach for detection of Encephalitozoon intestinalis, an emergent agent. Clin Vaccine Immunol 16:1021-1024
19. Libertin CR, Woloschak GE, Wilson WR, Smith TF (1984) Analysis of Pneumocystis carinii cysts with a fluorescence-activated cell sorter. J Clin Microbiol 20:877-880
20. Lapinsky SE, Glencross D, Car NG, Kallenbach JM, Zwi S (1991) Quantification and assessment of viability of Pneumocystis carinii organisms by flow cytometry. J Clin Microbiol 29:911-915
21. D'Avignon LC, Schofield CM, Hospenthal DR (2008) Pneumocystis pneumonia. Semin Respir Crit Care Med 29:132-140
22. Morris A, Wei K, Afshar K, Huang L (2008) Epidemiology and clinical significance of Pneumocystis colonization. J Infect Dis 197:10-17