Methods for the study of dopamine receptors within lipid rafts of kidney cells

Methods in Molecular Biology

Volumn 964, Issue , 2013, Pages 15-24

  Yu, Peiying ^a   Villar, Van Anthony ^a   Jose, Pedro A ^a  

^a GEORGE WASHINGTON UNIVERSITY SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

Author keywords

Caveolae; Dopamine receptor; Lipid rafts; Membrane microdomains

Indexed keywords

DETERGENT; DOPAMINE RECEPTOR;

ARTICLE; CELL FRACTIONATION; CELL MEMBRANE; CELL STRAIN HEK293; CYTOLOGY; DRUG EFFECT; HUMAN; IMMUNOBLOTTING; KIDNEY; METABOLISM; METHODOLOGY;

CELL FRACTIONATION; DETERGENTS; HEK293 CELLS; HUMANS; IMMUNOBLOTTING; KIDNEY; MEMBRANE MICRODOMAINS; RECEPTORS, DOPAMINE;

MLCS; MLOWN;

EID: 84878637945     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-251-3_2     Document Type: Article

References (21)

1. Jose PA, Eisner GM, Felder RA (2002) Role of dopamine receptors in the kidney in the regulation of blood pressure. Curr Opin Nephrol Hypertens 11:87-92
2. Holmes A, Lachowicz JE, Sibley DR (2004) Phenotypic analysis of dopamine receptor knockout mice; recent insights into the functional specificity of dopamine receptor subtypes. Neuropharmacology 47: 1117-1134
3. Kong MMC, Hasbi A, Mattocks M, Fan T, O'Dowd BF, George SR (2007) Regulation of D1 dopamine receptor trafficking and signaling by caveolin-1. Mol Pharmacol 72:1157-1170
4. Simons K, Ikonen E (1997) Functional rafts in cell membranes. Nature 387:569-572
5. Insel PA, Head BP, Ostrom RS, Patel HH, Swaney JS, Tang CM, Roth DM (2005) Caveolae and lipid rafts: G protein-coupled receptor signaling microdomains in cardiac myocytes. Ann N Y Acad Sci 1047:166-172
6. Lingwood D, Simons K (2010) Lipid rafts as a membrane-organizing principle. Science 327:46-50
7. Schnitzer JE, McIntosh DP, Dvorak AM, Liu J, Oh P (1995) Separation of caveolae from associated microdomains of GPI-anchored proteins. Science 269:1435-1439
8. Smart EJ, Ying YS, Mineo C, Anderson RG (1995) A detergent-free method for purifying caveolae membrane from tissue culture cells. Proc Natl Acad Sci U S A 92:10104-10108
9. Song KS, Li S, Okamoto T, Quilliam LA, Sargiacomo M, Lisanti MP (1996) Co-purification and direct interaction of Ras with caveolin, an integral membrane protein of caveolae microdomains. Detergent-free purification of caveolae microdomains. J Biol Chem 271:9690-9697
10. Foster LJ, De Hoog CL, Mann M (2003) Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors. Proc Natl Acad Sci U S A 100:5813-5818
11. Salzer U, Prohaska R (2001) Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts. Blood 97:1141-1143
12. Huang P, Xu W, Yoon SI, Chen C, Chong PL, Liu-Chen LY (2007) Cholesterol reduction by methyl-beta-cyclodextrin attenuates the delta opioid receptor-mediated signaling in neuronal cells but enhances it in non-neuronal cells. Biochem Pharmacol 73:534-549
13. Yu P, Yang Z, Jones JE, Wang Z, Owens SA, Mueller SC, Felder RA, Jose PA (2004) D1 dopamine receptor signaling involves caveolin- 2 in HEK-293 cells. Kidney Int 66:2167-2180
14. Breton S, Lisanti MP, Tyszkowski R, McLaughlin M, Brown D (1998) Basolateral distribution of caveolin-1 in the kidney. Absence from H+-ATPase-coated endocytic vesicles in intercalated cells. J Histochem Cytochem 46:205-214
15. Pike LJ (2004) Lipid rafts: heterogeneity on the high seas. Biochem J 378:281-292
16. Waheed AA, Jones TL (2002) Hsp90 interactions and acylation target the G protein G-12 but not G-13 to lipid rafts. J Biol Chem 277:32409-32412
17. Verkade P, Harder T, Lafont F, Simons K (2000) Induction of caveolae in the apical plasma membrane of Madin-Darby canine kidney cells. J Cell Biol 148:727-739
18. Macdonald JL, Pike LJ (2005) A simplified method for the preparation of detergent-free lipid rafts. J Lipid Res 46:1061-1067
19. Vial C, Evans RJ (2005) Disruption of lipid rafts inhibits P2X1 receptor-mediated currents and arterial vasoconstriction. J Biol Chem 280:30705-30711
20. Murtazina R, Kovbasnjuk O, Donowitz M, Li X (2006) Na+/H+ exchanger NHE3 activity and trafficking are lipid raft-dependent. J Biol Chem 281:17845-17855
21. Madre N, Smith KL, Graham CH, Jen A, Brady K, Hall S, Morris R (1999) Functionally different GPI proteins are organized in different domains on the neuronal surface. EMBO J 18:6917-6926