Microscopic detection of yeasts using fluorescence in situ hybridization

Methods in Molecular Biology

Volumn 968, Issue , 2013, Pages 71-82

  Inácio, João ^a   Da Luz Martins, Maria ^a  

^a INSTITUTO DE HIGIENE E MEDICINA TROPICAL   (Portugal)

Author keywords

Cryptococcus neoformans; Cy3 labeled DNA probes; Epi fl uorescence microscopy; Fluorescence in situ hybridization; Ribosomal RNA; Yeast detection and identification

Indexed keywords

FLUOROCHROME; PARAFORMALDEHYDE;

ARTICLE; CEREBROSPINAL FLUID ANALYSIS; CRYPTOCOCCUS NEOFORMANS; EPIFLUORESCENCE MICROSCOPY; FLUORESCENCE IN SITU HYBRIDIZATION; FUNGAL CELL; FUNGAL DETECTION; FUNGUS CULTURE; FUNGUS IDENTIFICATION; INCUBATION TIME; NONHUMAN; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; AIDS RELATED COMPLEX; CEREBROSPINAL FLUID; CULTURE TECHNIQUE; CYTOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY;

AIDS-RELATED OPPORTUNISTIC INFECTIONS; CEREBROSPINAL FLUID; CRYPTOCOCCUS NEOFORMANS; CULTURE TECHNIQUES; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE;

MLCS; MLOWN;

EID: 84878626169     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-257-5_5     Document Type: Article

References (18)

1. Amann R, Ludwig W, Schleifer K-H (1995) Phylogenetic identi fication and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143-169
2. Moter A, Göbel UB (2000) Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. J Microbiol Methods 41:85-112
3. Zwirglmaier K (2005) Fluorescence in situ hybridisation (FISH) -The next generation. FEMS Microbiol Lett 246:151-158
4. Alexander BD, Ashley ED, Reller LB et al (2006) Cost savings with implementation of PNA FISH testing for identi fication of Candida albicans in blood cultures. Diagn Microbiol Infect Dis 54:277-282
5. Martins ML, Ferreira AS, Sampaio A et al (2010) Direct and speci fic identi fication of Cryptococcus neoformans in biological samples using fl uorescently-labelled DNA probes. Eur J Clin Microbiol Infect Dis 29:571-576
6. Stender H, Kurtzman C, Hyldig-Nielsen JJ et al (2001) Identi fication of Dekkera bruxellensis (Brettanomyces) from wine by fl uorescence in situ hybridization using peptide nucleic acid probes. Appl Environ Microbiol 67:938-941
7. Tavares S, Inácio J, Fonseca Á et al (2004) Direct detection of Taphrina deformans on peach trees using molecular methods. Eur J Plant Pathol 110:973-982
8. Xufre A, Albergaria H, Inácio J et al (2006) Application of fl uorescence in situ hybridisation (FISH) to the analysis of yeast population dynamics in winery and laboratory grape must fermentations. Int J Food Microbiol 108: 376-384
9. Andrews JH, Spear RN, Nordheim EV (2002) Population biology of Aureobasidium pullulans on apple leaf surfaces. Can J Microbiol 48:500-513
10. Li S, Spear RN, Andrews JH (1997) Quantitative fl uorescence in situ hybridization of Aureobasidium pullulans on microscopic slides and leaf surfaces. Appl Environ Microbiol 63:3261-3267
11. Inácio J, Ludwig W, Spencer-Martins I et al (2010) Assessment of phylloplane yeasts on selected Mediterranean plants by fl uorescence in situ hybridisation (FISH) with group-and species-speci fic oligonucleotide probes. FEMS Microbiol Ecol 71:61-72
12. Behrens S, Rühland C, Inácio J et al (2003) In situ accessibility of small subunit ribosomal RNA of members of the domains bacteria, archaea and eucarya to Cy3-labeled oligonucleotide probes. Appl Environ Microbiol 69:1748-1758
13. Inácio J, Behrens S, Fuchs BM et al (2003) In situ accessibility of Saccharomyces cerevisiae 26S rRNA to Cy3-labelled oligonucleotide probes comprising the D1 and D2 domains. Appl Environ Microbiol 69:2899-2905
14. Casadevall A, Perfect JR (1998) Cryptococcus neoformans . ASM Press Books, Washington, DC
15. Amann R, Krumholz L, Stahl DA (1990) Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol 172:762-770
16. Fuchs BM, Wallner G, Beisker W et al (1998) Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fl uorescently labeled oligonucleotide probes. Appl Environ Microbiol 64:4973-4982
17. Fuchs BM, Syutsubo K, Ludwig W et al (2001) In Situ accessibility of Escherichia coli 23S rRNA to fl uorescently labeled oligonucleotide probes. Appl Environ Microbiol 67:961-968
18. Tavares A, Inácio J, Melo-Cristino J et al (2008) Use of fl uorescence in situ hybridization for rapid identi fication of staphylococci in blood culture samples collected in a Portuguese hospital. J Clin Microbiol 46:3097-3100