Nanoliter high-throughput PCR for DNA and RNA profiling

Methods in Molecular Biology

Volumn 496, Issue , 2009, Pages 161-174

  Brenan, Colin J H ^a   Roberts, Douglas ^a   Hurley, James ^a  

^a BioTrove Inc   (United States)

Author keywords

5 exonuclease assay; High throughput genotyping; Nanofluidic; Nanoliter PCR; Quantitative PCR; SNP genotyping; SYBR Green I real time PCR; TaqMan PCR

Indexed keywords

DNA; RNA;

ANIMAL; GENE EXPRESSION PROFILING; GENETICS; HUMAN; METHODOLOGY; POLYMERASE CHAIN REACTION; REVIEW; SENSITIVITY AND SPECIFICITY; SINGLE NUCLEOTIDE POLYMORPHISM;

ANIMALS; DNA; GENE EXPRESSION PROFILING; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; RNA; SENSITIVITY AND SPECIFICITY;

EID: 58149312870     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-553-4_12     Document Type: Article

References (18)

1. Murray, S., Oliphant, A., Shen, R., McBride, C., Steeke, R., Shannon, S., Rubano, T., Kermani, B., Fan, J-B., Chee, M., Hansen, M. (2004) A highly informative SNP linkage panel for human genetic studies. Nature Meth 1, 113-117.
2. Saito, K., Nakayama T., Sato N., Morita A., Takahashi T., Soma M., Usami, R. (2006) Haplotypes of the plasminogen activator gene associated with ischemic stroke. Thromb Haemost 96, 331-336.
3. Shi, M. (2001) Enabling large-scale pharmacogenetic studies by high-throughput mutation detection and genotyping technologies. Clin Chem 47, 164-172.
4. Beuselinck K., van Ranst M., van Eldere, J. (2005) Automated extraction of viralpathogen RNA and DNA for high-throughput quantitative real-time PCR. J Clin Microbiol 43, 5541-5546.
5. Balashov S. V., Gardiner R., Park S., Perlin D. S. (2005) Rapid, high-throughput, multiplex, real-time PCR for identification of mutations in the cyp51A gene of Aspergillus fumigatus that confer resistance to itraconazaole. J Clin Microbiol 43, 214-222.
6. Stephens, A. J., Huygens F., Inman-Bamber J., Price E. P., Nimmo G. R., Schooneveldt J., Munckhof W., Giffard P. M. (2006) Methicillin-resistant Staphylococcus aureus gentoytping using a small set of polymorphisms. J Med Microbiol 55, 43-51.
7. Denomme G. A., Van Oene, M. (2005) High-throughput multiplex single-nucleotide polymorphism analysis for red cell and platelet antigen genotypes. Transfusion 45, 660-666.
8. Higgins, M., Hughes, A., Buzzacott, N., Lown J. (2004) High-throughput genotyping of human platelet antigens using the 5′-nuclease assay and minor groove binder probe technology. Vox Sang 87, 114-117.
9. Smith, C. T., Baker, J., Park, L., Seeb, W., Elfstrom, C., Abe, S., Seeb, J. E. (2005) Characterization of 13 single nucleotide polymorphism markers for chum salmon. Molecular Ecology Notes 5, 259-262.
10. Smith, C. T., Templin, W. D., Seeb, J. E., Seeb, L. W. (2005) Single nucleotide polymorphisms provide rapid and accurate estimates of the proportions of U.S. and Canadian chinook salmon caught in Yukon river fisheries. North Am J Fisheries Management 25, 944-953.
11. Giancola, S., McKhann, H., Berard, A., Camilleri, C., Durand, S., Libeau, P., Roux, F., Reboud, X., Gut, I., Brunel, D. (2006) Utilization of the three high-throughput SNP genotyping methods, the GOOD assay, Amplifluor and TaqMan, in diploid and polyploidy plants. Theoretical Appl Genetics 112, 1115-1124.
12. Liu, R. H. et al. (2004) Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection. Anal Chem 76, 1824-1831.
13. Lee, D. -S. et al. (2004) Bulk-micromachined submicroliter-volume PCR chip with very rapid thermal response and low power consumption. Lab Chip 4, 401-407.
14. Zhang, C., Xing, D. (2007) Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends Nucleic Acids Research 35, 4223-4237.
15. Dahl, A. et al. (2007) Quantitative PCR based expression analysis on a nanoliter scale using polymer nano-well chips Biomed Microdevices 9, 307-314.
16. Kanigan T. K., Brenan C. J. H., Lafontaine S., et al. (2000) Living chips for drug discovery. SPIE Proceedings 3929, 172-180.
17. Brenan C. J, H., Morrison T., Stone K., et al. (2002) A massively parallel microfluidics platform for storage and ultra high throughput screening. SPIE Proceedings 4626, 560-569.
18. Morrison T., Hurley J., Garcia J., et al. (2006) Nanoliter high throughput quantitative PCR. Nucleic Acids Res 34, e123.