Activities of acyl-CoA:Diacylglycerol acyltransferase (DGAT) and phospholipid:Diacylglycerol acyltransferase (PDAT) in microsomal preparations of developing sunflower and safflower seeds

Planta

Volumn 237, Issue 6, 2013, Pages 1627-1636

  Banaś, Walentyna ^a   Sanchez Garcia, Alicia ^b   Banaś, Antoni ^c   Stymne, Sten ^d  

^a SIEDLCE UNIVERSITY OF NATURAL SCIENCES AND HUMANITIES   (Poland)

^b INSTITUTO DE LA GRASA   (Spain)

^c MEDICAL UNIVERSITY OF GDANSK   (Poland)

^d SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

DGAT; Lipids; Microsomal preparation; PDAT; Safflower; Sunflower; Triacylglycerols

Indexed keywords

ACYLTRANSFERASE; DIACYLGLYCEROL ACYLTRANSFERASE; FATTY ACID; PHOSPHOLIPID DIACYLGLYCEROL ACYLTRANSFERASE; TRIACYLGLYCEROL;

ARTICLE; BIOLOGICAL MODEL; ENZYME SPECIFICITY; ENZYMOLOGY; GROWTH, DEVELOPMENT AND AGING; LIPID METABOLISM; METABOLISM; MICROSOME; PLANT SEED; SAFFLOWER; SUNFLOWER;

ACYLTRANSFERASES; CARTHAMUS TINCTORIUS; DIACYLGLYCEROL O-ACYLTRANSFERASE; FATTY ACIDS; HELIANTHUS; LIPID METABOLISM; MICROSOMES; MODELS, BIOLOGICAL; SEEDS; SUBSTRATE SPECIFICITY; TRIGLYCERIDES;

CARTHAMUS TINCTORIUS; HELIANTHUS;

EID: 84878361100     PISSN: 00320935     EISSN: 14322048     Source Type: Journal    
DOI: 10.1007/s00425-013-1870-8     Document Type: Article

References (38)

1. Banas A, Dahlqvist A, Stahl U, Lenman M, Stymne S (2000) The involvement of phospholipid: diacylglycerol acyltransferases in triacylglycerol production. Biochem Soc Trans 28: 703-705.
2. Bates PD, Browse J (2012) The significance of different diacylgycerol synthesis pathways on plant oil composition and bioengineering. Front Plant Sci 3: 147.
3. Blight EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Med Sci 37: 911-917.
4. Bouvier-Nave P, Benvenise P, Oelkers P, Sturley S, Schaller H (2000) Expression in yeast and tobacco of plant cDNAs encoding acyl-CoA: diacylglycerol acyltransferase. Eur J Biochem 267: 85-96.
5. Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E, Novak S, Collins C, Welch CB, Luis AJ, Erickson SK, Farese RV (1998) Identification of a gene encoding an acyl-CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc Natl Acad Sci USA 95: 13018-13023.
6. Chapman KD, Dyer JM, Mullen RT (2012) Biogenesis and functions of lipid droplets in plants: thematic review series: lipid droplet synthesis and metabolism: from yeast to man. J Lipid Res 53: 215-226.
7. Dahlqvist A, Ståhl U, Lenman M, Banas A, Lee M, Sandagar L, Ronne H, Stymne S (2000) Phospholipid: diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci USA 97: 6487-6492.
8. Ghosal A, Banas A, Ståhl U, Dahlqvist A, Lindqvist Y, Stymne S (2007) Saccharomyces cerevisiae phospholipid: diacylglycerol acyl transferase (PDAT) devoid of its membrane anchor region is a soluble and active enzyme retaining its substrate specificities. Biochim Biophys Acta 1771: 1457-1463.
9. Hernández ML, Whitehead L, He Z, Gazda V, Gilday A, Kozhevnikova E, Vaistij FE, Larson TR, Graham IA (2012) A cytosolic acyltransferase contributes to triacylglycerol synthesis in sucrose-rescued Arabidopsis seed oil catabolism mutants. Plant Physiol 160: 215-225.
10. Hobbs DH, Lu C, Hills MJ (1999) Cloning of a cDNA encoding diacylglycerol acyltransferase from Arabidopsis thaliana and its functional expression. FEBS Lett 452: 145-149.
11. Huang AHC (1992) Oil bodies and oleosins in seeds. Annu Rev Plant Physiol Plant Mol Biol 43: 177-200.
12. Kanda P, Wells MA (1986) Dihexanoyl phosphatidylethanolamine: effect of a head group charge on rates of alkaline and phospholipase-a2 catalyze dehydrolyzes. Chem Phys Lipids 39: 31-39.
13. Kennedy EP (1961) Biosynthesis of complex lipids. Fed Proc Fed Am Soc Exp Biol 20: 934-940.
14. Kroon JTM, Wei WX, Simon WJ, Slabas AR (2006) Identification and functional expression of type 2 acyl-CoA: diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds which has high homology to the major triglyceride biosynthetic enzyme of fungi and animals. Phytochemistry 67: 2541-2549.
15. Lardizabal KD, Mai JT, Wagner NW, Wyrick A, Voelker T, Hawkins DJ (2001) DGAT2 is a new diacylglycerol acyltransferase gene family-purification, cloning, and expression in insect cells of two polypeptides from Mortiella ramaniana with diacylglycerol acyltransferases activity. J Biol Chem 276: 38862-38869.
16. Liu Q, Siloto RM, Lehner R, Stone SJ, Weselake RJ (2012) Acyl-CoA: diacylglycerol acyltransferase: molecular biology, biochemistry and biotechnology. Prog Lipid Res 51: 350-377.
17. Lu C, Xin Z, Ren Z, Miquel M, Browse J (2009) An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis. Proc Natl Acad Sci USA 106: 18837-18842.
18. Mhaske V, Beldjilali K, Ohlrogge J, Pollard M (2005) Isolation and characterization of an Arabidopsis thaliana knockout line for phospholipid: diacylglycerol transacylase gene (At5g13640). Plant Physiol Biochem 43: 413-417.
19. Murphy DJ (ed) (2005) Plant lipids: biology, utilization and manipulation. Blackwell Publishing, Oxford.
20. Oelkers P, Tinkelenberg A, Erdeniz N, Cromley D, Billheimer JT, Sturley SL (2000) A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeast. J Biol Chem 275: 15609-15612.
21. Ohlrogge JB, Browse J (1995) Lipid biosynthesis. Plant Cell 7: 957-970.
22. Pérez-Vich B, Fernándes-Martinez JM, Grodona M, Knapp SJ, Berry ST (2002) Stearoyl-ACP and oleoyl-PC desaturase genes cosegregate with quantitative trait loci underlying high stearic and high oleic acid mutant phenotypes in sunflower. Theor Appl Genet 104: 338-349.
23. Saha S, Enugutti B, Rajakumari S, Rajasekharan R (2006) Cytosolic triacylglycerol biosynthetic pathway in oilseeds: molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase. Plant Physiol 141: 1533-1543.
24. Shockey JM, Gidda SK, Chapital DC, Kuan JC, Dhanoa PK, Bland JM, Rothstein SJ, Mullen RT, Dyer JM (2006) Tung tree GDAT1 and DGAT2 have non redundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell 18: 2294-2313.
25. Somerville C, Browse J, Jaworski JG, Ohlrogge JB (2001) Lipids. In: Buchanan BB, Gruissem W, Jones JL (eds) Biochemistry and molecular biology of plants. American Society of Plant Biologists, Rockville, pp 456-527.
26. Sörensen BM, Furukawa-Stoffer TL, Marshall KS, Page EK, Mir Z, Forster RJ, Weselake RJ (2005) Storage lipid accumulation and acyltransferase action in developing flax seeds. Lipids 40: 1043-1048.
27. Ståhl U, Carlsson A, Lenman M, Dahlqvist A, Huang B, Banas W, Banas A, Stymne S (2004) Cloning and functional characterization of a phospholipid: diacylglycerol acyltransferase from Arabidopsis. Plant Physiol 135: 1324-1335.
28. Stobart AK, Stymne S (1985) The regulation of fatty acid composition of the triacylglycerols in microsomal preparations from avocado mesocarp and developing cotyledons of safflower. Planta 163: 119-125.
29. Stobart AK, Mancha M, Lenman M, Dahlqvist A, Stymne S (1997) Triacylglycerols are synthesised and utilized by transacylation reactions in microsomal preparations of developing safflower (Carthamus tinctorius L.) seeds. Planta 203: 58-66.
30. Stymne S, Appelqvist LA (1978) The biosynthesis of linoleate from oleoyl-CoA via oleoyl-phosphatidylcholine in microsomes of developing safflower seeds. Eur J Biochem 90: 223-229.
31. Stymne S, Stobart AK (1984) Evidence for the reversibility of the acyl-CoA: lysophosphatidylcholine acyltransferase in microsomal preparations from developing safflower (Carthamus tinctorius L.) cotyledons and rat liver. Biochem J 223: 305-314.
32. Stymne S, Stobart AK (1987) Triacylglycerol biosynthesis. In: Stumpf PK (ed) The biochemistry of plants, vol 9., Lipids: structure and functionAcademic Press, New York, pp 175-214.
33. Triki S, Ben Hamida J, Mazliak P (2000) Diacylglycerol acyltransferase in maturing sunflower seeds. Biochem Soc Trans 28: 689-692.
34. Vogel G, Browse J (1996) Cholinephosphotransferase and diacylglycerol acyltransferase. Plant Physiol 110: 923-931.
35. Weselake RJ, Pomeroy MK, Furukawa TL, Golden JL, Little DB, Laroche A (1993) Developmental profile of diacylglycerol acyltransferase in maturing seeds of oilseed rape and safflower and in microspore derived cultures of oil rape. Plant Physiol 102: 565-571.
36. Wiberg E, Tillberg E, Stymne S (1994) Substrates of diacylglycerol acyltransferase in microsomes from developing seeds. Phytochemistry 36: 573-577.
37. Zhang M, Fan J, Taylor D, Ohlrogge J (2009) DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development. Plant Cell 21(12): 3885-3901.
38. Zou J, Wei Y, Jako C, Kumar A, Selvaraj G, Taylor DC (1999) The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene. Plant J 19: 645-653.