Molecular cloning, expression and purification of recombinant soluble mouse endostatin as an anti-angiogenic protein in Escherichia coli

Molecular Biology Reports

Volumn 40, Issue 2, 2013, Pages 1027-1033

  Yari, Kheirollah ^a   Afzali, Saboor ^a   Mozafari, Hadi ^a   Mansouri, Kamran ^a   Mostafaie, Ali ^a  

^a KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Angiogenesis; Endostatin; Escherichia coli; Molecular cloning

Indexed keywords

AMPICILLIN; ENDOSTATIN; HYBRID PROTEIN; RECOMBINANT ENDOSTATIN; THIOREDOXIN;

AGAR MEDIUM; ANIMAL TISSUE; ANTIANGIOGENIC ACTIVITY; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CLONING VECTOR; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA LIBRARY; DNA SEQUENCE; ENDOSTATIN GENE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE INSERTION; GENETIC STABILITY; IN VITRO STUDY; LIVER; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; UMBILICAL VEIN ENDOTHELIAL CELL;

ANGIOGENESIS INHIBITORS; ANIMALS; CAPILLARIES; CELLS, CULTURED; CLONING, MOLECULAR; DRUG SCREENING ASSAYS, ANTITUMOR; ENDOSTATINS; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; MICE; NEOVASCULARIZATION, PATHOLOGIC; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84878355633     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-012-2144-4     Document Type: Article

References (27)

1. Colorado PC, Torre A, Kamphaus G, Maeshima Y, Hopfer H, Takahashi K, Volk R, Zamborsky ED, Herman S, Sarkar PK, Ericksen MB, Dhanabal M, Simons M, Post M, Kufe DW, Weichselbaum RR, Sukhatme VP, Kalluri R (2000) Anti-angiogenic cues from vascular basement membrane collagen. Cancer Res 60(9):2520-2526
2. Mansouri K, Khodarahmi R, Foroumadi A, Mostafaie A, Mohammadi Motlagh HR (2011) Anti-angiogenic/proliferative behavior of a "4-aryl-4H- chromene" on blood vessel's endothelial cells: a possible evidence on dual "anti-tumor" activity. Med Chem Res 20(7):920-929
3. Mostafaie A, Mansouri K, Norooznezhad AH, Mohammadi-Motlagh HR (2011) Anti-angiogenic activity of ficus carica latex extract on human umbilical vein endothelial cells. Yakhteh 12(4):525-528
4. Boosani CS, Sudhakar A (2006) Cloning, purification, and characterization of a non-collagenous anti-angiogenic protein domain from human alpha 1 type IV collagen expressed in Sf9 cells. Protein Expr Purif 49(2):211-218. doi: 10.1016/j.pep.2006.03.007
5. Huang XJ, Wong MKK, Zhao Q, Zhu ZY, Wang KZQ, Huang N, Ye CS, Gorelik E (2001) Li MF (2001) Soluble recombinant endostatin purified from Escherichia coli: antiangiogenic activity and antitumor effect (vol 61, pg 478). Cancer Res 61(15):5956
6. Deininger MH, Wybranietz WA, Graepler FTC, Lauer UM, Meyermann R, Schluesener HJ (2003) Endothelial endostatin release is induced by general cell stress and modulated by the nitric oxide/cGMP pathway. Faseb J 17(10):1267-1276. doi: 10.1096/fj.02-1118com
7. Sorensen DR, Read TA, Porwol T, Olsen BR, Timpl R, Sasaki T, Iversen PO, Benestad HB, Sim BKL, Bjerkvig R (2002) Endostatin reduces vascularization, blood flow, and growth in a rat gliosarcoma. Neuro-Oncology 4(1):1-8
8. Reijerkerk A, Mosnier LO, Kranenburg O, Bouma BN, Carmeliet P, Drixler T, Meijers JCM, Voest EE, Gebbink MFBG (2003) Amyloid endostatin induces endothelial cell detachment by stimulation of the plasminogen activation system. Mol Cancer Res 1(8):561-568
9. Tanya AR, Constance YF, Candece LG (2005) Endogenous inhibitors of angiogenesis in malignant gliomas: nature's antiangiogenic therapy. Neuro-Oncology 7(2):106-121
10. Wickstrom SA, Alitalo K, Keski-Oja J (2005) Endostatin signaling and regulation of endothelial cell-matrix interactions. Adv Cancer Res 94:197-229. doi: 10.1016/S0065-230x(04)94005-5
11. Kahrizi D, Salmanian AH, Afshari A, Moieni A, Mousavi A (2007) Simultaneous substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E-coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate. Plant Cell Rep 26(1):95-104. doi: 10.1007/s00299-006-0208-4
12. Yari K, Fatemi SS, Tavallaei M (2010) Optimization of the BoNT/A-Hc expression in recombinant Escherichia coli using the Taguchi statistical method. Biotechnol Appl Biochem 56(1):35-42. doi: 10.1042/BA20090315
13. Wu XP, Li XK, Su ZJ, Zheng Q, Xu H, Wu SX, Feng Y, Zhao W (2005) Expression and purification of soluble non-fusion vasostatin in Escherichia coli. Protein Pept Lett 12(7):659-661
14. Mirmoayedi A, Kahrizi D, Ebadi AA, Yari K, Mohammadi M (2012) Study of individual and sex genetic diversity among each genus and between two genera of Chrysopa and Chrysoperla (Neuroptera, Chrysopidae) based on RAPD-PCR polymorphism. Mol Biol Rep 39(9):8999-9006. doi: 10.1007/s11033-012-1770-1
15. Yari K, Mostafaie A (2011) Determination of suitable conditions for great plasmid transformation into Escherichia coli using Taguchi statistical method. Am J Sci Res 17:151-157
16. Yari K, Fatemi SSA, Tavallaei M (2012) High level expression of recombinant BoNT/A-Hc by high cell density cultivation of Escherichia coli. Bioprocess Biosyst Eng 35(3):407-414. doi: 10.1007/s00449-011-0579-y
17. Babaeipour V, Shojaosadati SA, Robatjazi SM, Khalilzadeh R, Maghsoudi N (2007) Over-production of human interferon-gamma by HCDC of recombinant Escherichia coli. Process Biochem 42(1):112-117. doi: 10.1016/j.procbio.2006.07. 009
18. Sambrook J, Fristsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
19. Mostafaie A, Yari K, Kiani S (2011) A comparative evaluation of rehydration and cup-loading sample application for modified two-dimensional gel electrophoresis of human serum proteins using immobilized pH gradient. Afr J Biotech 10(55):11711-11715
20. Kakaei M, Kahrizi D, Mostafaie A (2010) Study on powdery mildew disease related proteins expression in winter wheat cultivars via SDS-PAGE. Biharean Biologist 4(2):169-171
21. Deplanque G, Harris AL (2000) Anti-angiogenic agents: clinical trial design and therapies in development. Eur J Cancer 36(13 Spec No):1713-1724
22. Folkman J (2006) Antiangiogenesis in cancer therapy - endostatin and its mechanisms of action. Exp Cell Res 312(5):594-607. doi: 10.1016/j.yexcr.2005.11. 015
23. Ortega N, Werb Z (2002) New functional roles for non-collagenous domains of basement membrane collagens. J Cell Sci 115(22):4201-4214. doi: 10.1242/Jcs.00106
24. Dhanabal M, Ramchandran R, Waterman MJF, Lu H, Knebelmann B, Segal M, Sukhatme VP (1999) Endostatin induces endothelial cell apoptosis. J Biol Chem 274(17):11721-11726
25. Rehn M, Veikkola T, Kukk-Valdre E, Nakamura H, Ilmonen M, Lombardo CR, Pihlajaniemi T, Alitalo K, Vuori K (2001) Interaction of endostatin with integrins implicated in angiogenesis. Proc Natl Acad Sci USA 98(3):1024-1029
26. Zhan JB, Chen XY, Wang CM, Qiu JW, Ma FS, Wang KY, Zheng S (2003) A fusion protein of conotoxin MVIIA and thioredoxin expressed in Escherichia coli has significant analgesic activity. Biochem Biophys Res Commun 311(2):495-500. doi: 10.1016/j.bbrc.2003.09.234
27. Xu HM, Zhang GY, Ji XD, Cao L, Shu L, Hua ZC (2005) Expression of soluble, biologically active recombinant human endostatin in Escherichia coli. Protein Expr Purif 41(2):252-258. doi: 10.1016/j.pep.2004.09.021