High level expression of recombinant BoNT/A-Hc by high cell density cultivation of Escherichia coli

Bioprocess and Biosystems Engineering

Volumn 35, Issue 3, 2012, Pages 407-414

  Yari, Kheirollah ^a,b   Fatemi, Seyed Safa Ali ^b   Tavallaei, Mahmood ^c  

^a KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

^c BAQIYATALLAH UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Clostridium botulinum; Escherichia coli; Fed batch; High cell density cultivation; Taguchi method

Indexed keywords

CLOSTRIDIUM BOTULINUM; CLOSTRIDIUM BOTULINUM NEUROTOXIN; FED BATCHES; HEAVY CHAIN; HIGH LEVEL EXPRESSION; HIGH-CELL-DENSITY CULTIVATIONS; OPTIMUM CONDITIONS; PROTEIN EXPRESSIONS; RECOMBINANT ESCHERICHIA COLI; SHAKE FLASKS; STATISTICAL DESIGN; TAGUCHI;

ANTIGENS; CYTOLOGY; ESCHERICHIA COLI; TAGUCHI METHODS;

CELLS;

BOTULINUM TOXIN A; RECOMBINANT BOTULINUM TOXIN A HEAVY CHAIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BIOREACTOR; CELL DENSITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; STATISTICAL ANALYSIS;

BIOREACTORS; BOTULINUM TOXINS, TYPE A; CLOSTRIDIUM BOTULINUM; ESCHERICHIA COLI; RECOMBINANT PROTEINS; TOXOIDS;

CLOSTRIDIUM BOTULINUM; ESCHERICHIA COLI;

EID: 84857359655     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-011-0579-y     Document Type: Article

References (22)

1. H Zhou B Zhou EA Johenson KD Janda 2009 Selection and characterization of a human monoclonal neutralizing antibody for Clostridium botulinum neurotoxin serotype B Bioorg Med Chem Lett 19 662 664 10.1016/j.bmcl.2008.12.055 1:CAS:528:DC%2BD1MXhtF2isrw%3D
2. YZ Yu N Li HQ Zhu RL Wang Y Du S Wang WY Yu ZW Sun 2009 The recombinant Hc subunit of Clostridium botulinum neurotoxin serotype A is an effective botulism vaccine candidate Vaccine 27 2816 2822 10.1016/j.vaccine.2009.02.091 1:CAS:528:DC%2BD1MXkslOhsbo%3D
3. L Li R Bal 1999 High-level expression, purification, and characterization of recombinant type A botulinum neurotoxin light chain Protein Expr Purif 17 339 344 10.1006/prep.1999.1138 1:CAS:528:DyaK1MXnvFaqtrs%3D
4. RC Lin RH Scheller 2000 Mechanism of synaptic vesicle exocytosis Annu Rev Cell Dev Biol 16 19 49 10.1146/annurev.cellbio.16.1.19 1:CAS:528:DC%2BD3MXpvFOi (Pubitemid 32037499)
5. HF Lapenotiere MA Clayton JL Middlebrook 1995 Expression of a large, nontoxic fragment of botulinum neurotoxin serotype A and its use as an immunogen Toxicon 33 1383 1386 10.1016/0041-0101(95)00072-T 1:CAS:528:DyaK2MXptVantbc%3D
6. M Tavallaie A Chenal D Gillet Y Pereira M Manich M Gibert S Raffestin MR Popoff JCH Marvaud 2004 Interaction between two sub domain of the c-terminal part of the BON/A is essential for generation of protective antibodies FEBS Lett 572 299 306 10.1016/j.febslet.2004.06.094 1:CAS:528:DC%2BD2cXmsVGhtL8%3D (Pubitemid 39092553)
7. DD Pless ER Toress EK Reinke S Bavari 2001 High-affinity, protective antibody to the binding domain of botulinum neurotoxin type A Infect Immun 69 570 574 10.1128/IAI.69.1.570-574.2001 1:CAS:528:DC%2BD3MXitVaqtw%3D%3D (Pubitemid 32040950)
8. R Chen J Shi K Cai W Tu X Hou H Liu L Xiao Q Wang Y Tang H Wang 2009 Improved soluble expression of the Hc domain of Clostridium botulinum neurotoxin serotype A in Escherichia coli by using a PCR-synthesized gene and a Trx co-expression strain Protein Expr Purif 71 79 84 10.1016/j.pep.2009.11.007
9. J Choi K Keum S Lee 2006 Production of recombinant proteins by high cell density culture of Escherichia coli Chem Eng Sci 61 876 885 10.1016/j.ces.2005. 03.031 1:CAS:528:DC%2BD2MXht1aqur3J (Pubitemid 41650182)
10. O Durany C Mas J Lopez-Santin 2005 Fed-batch production of recombinant fuculose-1-phosphate aldolase in E. coli Process Biochem 40 707 716 10.1016/j.procbio.2004.01.058 1:CAS:528:DC%2BD2cXpt1Wgsb0%3D (Pubitemid 39437872)
11. K Yari SSA Fatemi M Tavallaei 2010 Optimization of the BoNT/A-Hc expression in recombinant Escherichia coli using Taguchi statistical method Biotechnol Appl Biochem 56 35 42 10.1042/BA20090315 1:CAS:528: DC%2BC3cXmsVGktLc%3D
12. SA Shojaosadati SM Varedi Kolaei V Babaeipour AM Farnoud 2008 Recent advances in high cell density cultivation for production of recombinant protein Iranian J Biotech 6 63 84 1:CAS:528:DC%2BD1MXhvVGntb8%3D
13. KR Babu S Swaminathan S Marten N Khanna U Rinas 2000 Production of interferon-α in high cell density cultures of recombinant Escherichia coli and its single step purification from refolded inclusion body proteins Appl Microbiol Biotechnol 53 655 660 10.1007/s002530000318 1:CAS:528: DC%2BD3cXkvVyhur0%3D (Pubitemid 30430219)
14. R Khalilzadeh SA Shojaosadati A Bahrami N Maghsoudi 2003 Over-expression of recombinant human interferon-gamma in high cell density fermentation of Escherichia coli Biotechnol Lett 25 1989 1992 10.1023/B:BILE.0000004390.98648.25 1:CAS:528:DC%2BD3sXptVCqtb8%3D (Pubitemid 37502439)
15. ST Vaiphei G Pandey KJ Mukherjee 2009 Kinetic studies of recombination human interferon-gamma expression in continuous cultures of E. coli J Ind Microbiol Biotechnol 36 1453 1458 10.1007/s10295-009-0632-x 1:CAS:528: DC%2BD1MXhtl2rt7vI
16. Sambrook J, Fristsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
17. T Matsui H Sato H Yamamuro S Misawa N Shinzato H Matsuda J Takahashi S Sato 2008 High cell density cultivation of recombinant Escherichia coli for hirudin variant 1 production J Biotechnol 134 88 92 10.1016/j.jbiotec.2007.12. 012 1:CAS:528:DC%2BD1cXislKktLw%3D
18. SSA Fatemi B Yakhchali J Towfighi Darian SA Shojaosadati A Zomorodipour AA Karkhane F Rasrgar Jazii 2003 Selection of a suitable strain from recombinant Escherichia coli strains with the same genetic structure expressing periplasmic hGM-CSF J Biosci Bioeng 96 578 580 10.1016/S1389-1723(04)70153-X 1:CAS:528:DC%2BD2cXhvVCktrk%3D (Pubitemid 38091234)
19. Y Liu XL Li DD Zhang Q Wang Y Liu DM Liu CT Wu CP Cui 2010 Production of hepatopoietin Cn in high-cell-density cultures of recombinant Escherichia coli and detection of its antioxygen activity Mol Biotechnol 47 111 119 10.1007/s12033-010-9318-x
20. V Babaeipour SA Shojaosadati SM Robatjazi R Khalilzadeh N Maghsoudi 2007 Over-production of human interferon-g by HCDC of recombinant Escherichia coli Process Biochem 42 112 117 10.1016/j.procbio.2006.07.009 1:CAS:528: DC%2BD28Xht1agsbbL (Pubitemid 44738654)
21. W Zhang K Potter AB Plantz LV Schlegel AL Smith MM Meagher 2003 Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement J Ind Microbiol Biotechnol 30 210 215 1:CAS:528:DC%2BD3sXjvFCjtr0%3D (Pubitemid 36693774)
22. JK Potter W Zhang AL Smith MM Meagher 2000 Production and purification of the heavy chain fragment C of botulinum neurotoxin, serotype A, expressed in the methylotrophic Yeast Pichia pastoris Protein Express Purif 19 393 402 10.1006/prep.2000.1256 1:CAS:528:DC%2BD3cXltVCitr8%3D