Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases

Journal of Biomolecular Screening

Volumn 18, Issue 5, 2013, Pages 621-628

  Yang, Wei ^a   Wang, Liangli ^a   Paschen, Wulf ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

AlphaScreen technology; assay development; inhibitor; SUMO; SUMO proteases

Indexed keywords

PEPTIDASE; PROTEIN INHIBITOR; SUMO 1 PROTEIN; SUMO 2 PROTEIN; SUMO 3 PROTEIN; MUTANT PROTEIN; PROTEINASE INHIBITOR; SUMO PROTEIN; SUMO2 PROTEIN, HUMAN; SUMO3 PROTEIN, HUMAN; UBIQUITIN;

ARTICLE; CONCENTRATION RESPONSE; ENZYME ACTIVITY; HIGH THROUGHPUT SCREENING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION; SUMOYLATION; WESTERN BLOTTING; ANTAGONISTS AND INHIBITORS; BIOLOGICAL MODEL; DOSE RESPONSE; DRUG EFFECTS; DRUG SCREENING; ENZYME SPECIFICITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; SENSITIVITY AND SPECIFICITY;

DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MODELS, BIOLOGICAL; MUTANT PROTEINS; PROTEASE INHIBITORS; SENSITIVITY AND SPECIFICITY; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SUBSTRATE SPECIFICITY; SUMOYLATION; UBIQUITINS;

EID: 84878188478     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057113479971     Document Type: Article

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