Isolation and characterization of a thermophilic Bacillus sp. with protease activity isolated from hot spring of Tarabalo, Odisha, India

Iranian Journal of Microbiology

Volumn 5, Issue 2, 2013, Pages 159-165

  Panda, Mrunmaya Kumar ^a   Sahu, Mahesh Kumar ^a   Tayung, Kumananda ^a  

^a North Orissa University ^*  (India)

Author keywords

G+c content; Phylogenetic analysis; Protease; Thermophilic Bacillus

Indexed keywords

CATALASE; PROTEINASE; RNA 16S;

ARTICLE; BACILLUS (ROD SHAPED BACTERIUM); BACILLUS AMYLOLIQUEFACIENS; BACTERIAL ENDOSPORE; BACTERIUM EXAMINATION; BACTERIUM ISOLATION; BIOCHEMISTRY; DNA SEQUENCE; ENZYME ASSAY; GENE SEQUENCE; GRAM POSITIVE BACTERIUM; HEAT TOLERANCE; MORPHOLOGICAL TRAIT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; THERMAL SPRING; THERMOPHILIC BACTERIUM;

EID: 84878034124     PISSN: 20083289     EISSN: 20084447     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Kellenberger E. Exploring the unknown: The silent revolution of microbiology. EMBO Rep 2001; 2: 5-7.
2. Haki GD, Rakshit SK. Developments in industrially important thermostable enzymes. Biores Technol 2003; 89: 17-34.
3. Coolbear T, Daniel R, Morgan HW. The enzymes from extreme thermophiles, bacterial sources, thermostability and industrial relevance. Adv Biochem Eng Biotechnol 1992; 45: 57-98.
4. Mala B, Rao AM, Deshpande VV. Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 1998; 62: 597-635.
5. Mohanta H, Rath C. Extracellular enzymatic activity of bacterial strains isolated from a local hotspring Tarabalo, Nayagarh District, Orissa, India. Internet J Microbiol 2010; 7: 2.
6. Sambrook J, Russell D (2001). Molecular Clon-ing: A laboratory manual. Cold Spring Harbor. NewYork.
7. Edwards U, Rogall T, Blocker H, Emde M and Bottger E C. Isolation and direct complete nucleotide determination of entire genes. Nucleic Acids Res 1989; 17: 7843-7853.
8. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24: 1596-1599.
9. Umashankar V, Vijayakumar A, Sudarsanam D. ACUA: A software tool for automated codon usage analysis. Bioinformation 2007; 2: 62-63.
10. Narayan VV, Hatha MA, Morgan HW, Rao D. Isolation and characterization of aerobic thermophilic bacteria from Savusavu hot springs in Fiji. Microbes Environ 2008; 23: 350-352.
11. Rath CC. Heat stable lipase activity of thermotolerant bacteria from hot springs at Orissa, India. Cytobios 1999; 99: 105-11.
12. Cowan D. Industrial enzyme technology. Trends Biotechnol 1996; 14: 177-178.
13. Guangrong H. Tiejing Y, Po H, Jiaxing J. Purification and characterization of a protease from Thermophilic Bacillus strain HS08. Afr J Biotechnol 2006; 5: 2433-2438.
14. Vijayalakshmi S, Venkatkumar S, Thankamani V. Screening of alkalophilic thermophilic protease isolated from Bacillus RV.B2.90 for industrial applications. Res Biotechnol 2011; 2: 32-41.
15. Babu NKS, Lakshmi KD. Optimization of thermostable alkaline protease production from species of Bacillus using rice bran. Afr J Biotechnol 2005; 4: 724-726.
16. Pastor MD, Lorda GS, Balatti. A Protease obtention using Bacillus subtilis 3411 and amaranth seed meal medium at different aeration rates. Braz J Microbiol 2001; 32: 1-8.
17. Wu H, Zhang Z, Hu H, and Yu J. On the molecular mechanism of GC content variation among eubacterial genomes. Biology Direct 2012; 7: 2.
18. Hurst LD, Merchant AR. High guanine-cytosine content is not an adaptation to high temperature: a comparative analysis amongst prokaryotes. Proc Biol Sci 2001; 268: 493-497.