A novel antithrombotic effect of sulforaphane via activation of platelet adenylate cyclase: Ex vivo and in vivo studies

Journal of Nutritional Biochemistry

Volumn 24, Issue 6, 2013, Pages 1086-1095

  Jayakumar, Thanasekaran ^a   Chen, Wei Fan ^a   Lu, Wan Jung ^a   Chou, Duen Suey ^a   Hsiao, George ^a   Hsu, Chung Yi ^b   Sheu, Joen Rong ^a   Hsieh, Cheng Ying ^a  

^a TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^b CHINA MEDICAL UNIVERSITY   (Taiwan)

Author keywords

Akt; Cyclic AMP; MAPK; Platelet activation; PLC 2; Sulforaphane

Indexed keywords

1H 1,2,4 OXADIAZOLO[4,3 A]QUINOXALIN 1 ONE; 2 [1 (3 AMIDINOTHIOPROPYL) 1H INDOL 3 YL] 3 (1 METHYL 1H INDOL 3 YL)MALEIMIDE; 2 MORPHOLINO 8 PHENYLCHROMONE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; 9 (TETRAHYDRO 2 FURYL)ADENINE; ADENYLATE CYCLASE; CALCIUM; COLLAGEN; CYCLIC AMP; CYCLIC GMP; HYDROXYL RADICAL; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; PHOSPHOLIPASE C GAMMA2; PROTEIN KINASE B; PROTEIN KINASE C; SULFORAPHANE; VASODILATOR STIMULATED PHOSPHOPROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CALCIUM MOBILIZATION; CANCER INHIBITION; CONTROLLED STUDY; DRUG MECHANISM; ENZYME ACTIVATION; EX VIVO STUDY; HUMAN; HUMAN CELL; IN VIVO STUDY; LUNG EMBOLISM; MOUSE; NONHUMAN; PROTEIN PHOSPHORYLATION; THROMBOCYTE ACTIVATION; THROMBOCYTE AGGREGATION; THROMBOCYTOPOIESIS;

ADENOSINE DIPHOSPHATE; ADENYLATE CYCLASE; ANIMALS; BLOOD PLATELETS; CELLS, CULTURED; CYCLIC AMP; CYCLIC GMP; ENZYME ACTIVATION; FIBRINOLYTIC AGENTS; HUMANS; ISOTHIOCYANATES; MICE; PHOSPHATIDYLINOSITOL 3-KINASES; PHOSPHOLIPASE C GAMMA; PLATELET ACTIVATION; PROTO-ONCOGENE PROTEINS C-AKT; SIGNAL TRANSDUCTION;

BRASSICA OLERACEA VAR. CAPITATA; BRASSICA OLERACEA VAR. ITALICA; MUS;

EID: 84878018724     PISSN: 09552863     EISSN: 18734847     Source Type: Journal    
DOI: 10.1016/j.jnutbio.2012.08.007     Document Type: Article

References (28)

1. Genkinger J.M., Platz E.A., Hoffman S.C., Comstock G.W., Helzlsouer K.J. Fruit, vegetable, and antioxidant intake and all-cause, cancer, and cardiovascular disease mortality in a community-dwelling population in Washington County, Maryland. Am J Epidemiol 2004, 160:1223-1233.
2. Zhang Y., Talalay P., Cho C.G., Posner G.H. A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure. Proc Natl Acad Sci USA 1992, 89:2399-2403.
3. Keum Y.S., Jeong W.S., Kong A.N. Chemoprevention by isothiocyanates and their underlying molecular signaling mechanisms. Mutat Res 2004, 555:191-202.
4. Myzakand M.C., Dashwood R.H. Histone deacetylases as targets for dietary cancer preventive agents: lessons learned with butyrate, diallyl disulfide, and sulforaphane. Curr Drug Targets 2006, 7:443-452.
5. Keum Y.S., Khor T.O., Lin W., Shen G., Kwon K.H., Barve A., et al. Pharmacokinetics and pharmacodynamics of broccoli sprouts on the suppression of prostate cancer in transgenic adenocarcinoma of mouse prostate (TRAMP) mice: implication of induction of Nrf2, HO-1 and apoptosis and the suppression of Akt-dependent kinase pathway. Pharm Res 2009, 26:2324-2331.
6. Sheu J.R., Lee C.R., Lin C.H., Hsiao G., Ko W.C., Chen Y.C., et al. Mechanisms involved in the antiplatelet activity of Staphylococcus aureus lipoteichoic acid in human platelets. Thromb Haemost 2000, 83:777-784.
7. von Hundelshausen P., Weber C. Platelets as immune cells: bridging inflammation and cardiovascular disease. Circ Res 2007, 100:27-40.
8. Rahman K. Effects of garlic on platelet biochemistry and physiology. Mol Nutr Food Res 2007, 51:1335-1344.
9. Sheu J.R., Chao S.H., Yen M.H., Huang T.F. In vivo antithrombotic effect of triflavin, an Arg-Gly-Asp containing peptide on platelet plug formation in mesenteric microvessels of mice. Thromb Haemost 1994, 72:617-621.
10. Hsiao G., Lin K.H., Chang Y., Chen T.L., Tzu N.H., Chou D.S., et al. Protective mechanisms of inosine in platelet activation and cerebral ischemic damage. Arterioscler Thromb Vasc Biol 2005, 25:1998-2004.
11. Sheu J.R., Hung W.C., Wu C.H., Ma M., Kan Y.C., Lin C.H., et al. Reduction in lipopolysaccharide-induced thrombocytopenia by triflavin in a rat model of septicemia. Circulation 1999, 99:3056-3062.
12. Chou D.S., Hsiao G., Shen M.Y., Tsai Y.J., Chen T.F., Sheu J.R. ESR spin trapping of a carbon-centered free radical from agonist-stimulated human platelets. Free Radic Biol Med 2005, 39:237-248.
13. Singer W.D., Brown H.A., Sternweis P.C. Regulation of eukaryotic phosphatidylinositol-specific phospholipase C and phospholipase D. Annu Rev Biochem 1997, 66:475-509.
14. Tyers M., Rachubinski R.A., Stewart M.I., Varrichio A.M., Shorr R.G., Haslam R.J., et al. Molecular cloning and expression of the major protein kinase C substrate of platelets. Nature 1988, 333:470-473.
15. Aszodi A., Pfeifer A., Ahmad M., Glauner M., Zhou X.H., Ny L., et al. The vasodilator-stimulated phosphoprotein (VASP) is involved in cGMP- and cAMP-mediated inhibition of agonist-induced platelet aggregation, but is dispensable for smooth muscle function. EMBO J 1999, 18:37-48.
16. Cheung K.L., Kong A.N. Molecular targets of dietary phenethyl isothiocyanate and sulforaphane for cancer chemoprevention. AAPS J 2010, 12:87-97.
17. Nordoy A., Chandler A.B. Platelet thrombosis induced by adenosine diphosphate in the rat. Scand J Haematol 1964, 1:16-25.
18. Sheu J.R., Hung W.C., Wu C.H., Lee Y.M., Yen M.H. Antithrombotic effect of rutaecarpine, an alkaloid isolated from Evodia rutaecarpa, on platelet plug formation in in vivo experiments. Br J Haematol 2000, 110:110-115.
19. Huang T.F., Sheu J.R., Teng C.M., Chen S.W., Liu C.S. Triflavin, an antiplatelet Arg-Gly-Asp-containing peptide, is a specific antagonist of platelet membrane glycoprotein IIb-IIIa complex. J Biochem 1991, 109:328-334.
20. Sheu J.R., Teng C.M., Huang T.F. Triflavin, an RGD-containing antiplatelet peptide, binds to GpIIIa of ADP-stimulated platelets. Biochem Biophys Res Commun 1992, 189:1236-1242.
21. Mangin P., Yuan Y., Goncalves I., Eckly A., Freund M., Cazenave J.P., et al. Signaling role for phospholipase C gamma 2 in platelet glycoprotein Ib alpha calcium flux and cytoskeletal reorganization. Involvement of a pathway distinct from FcR gamma chain and Fc gamma RIIA. J Biol Chem 2003, 278:32880-32891.
22. Pascale A., Amadio M., Govoni S., Battaini F. The aging brain, a key target for the future: the protein kinase C involvement. Pharmacol Res 2007, 55:560-569.
23. Ragab A., Severin S., Gratacap M.P., Aguado E., Malissen M., Jandrot-Perrus M., et al. Roles of the C-terminal tyrosine residues of LAT in GPVI-induced platelet activation: insights into the mechanism of PLC gamma 2 activation. Blood 2007, 110:2466-2474.
24. Bugaud F., Nadal-Wollbold F., Levy-Toledano S., Rosa J.P., Bryckaert M. Regulation of c-jun-NH2 terminal kinase and extracellular-signal regulated kinase in human platelets. Blood 1999, 94:3800-3805.
25. Hughes P.E., Renshaw M.W., Pfaff M., Forsyth J., Keivens V.M., Schwartz M.A., et al. Suppression of integrin activation: a novel function of a Ras/Raf-initiated MAP kinase pathway. Cell 1997, 88:521-530.
26. Coulon L., Calzada C., Moulin P., Vericel E., Lagarde M. Activation of p38 mitogen-activated protein kinase/cytosolic phospholipase A2 cascade in hydroperoxide-stressed platelets. Free Radic Biol Med 2003, 35:616-625.
27. Franke T.F., Yang S.I., Chan T.O., Datta K., Kazlauskas A., Morrison D.K., et al. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 1995, 81:727-736.
28. Walter U., Eigenthaler M., Geiger J., Reinhard M. Role of cyclic nucleotide-dependent protein kinases and their common substrate VASP in the regulation of human platelets. Adv Exp Med Biol 1993, 344:237-249.